MRPS15 antibody

Principal name

MRPS15 antibody

Alternative names for MRPS15 antibody

S15mt, RPMS15, DC37, 28S ribosomal protein S15, mitochondrial

SwissProt ID

P82913 (Bovin), P82914 (Human), Q9DC71 (Mouse)

Gene ID

64960 (MRPS15), 66407 (Mrps15)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of MRPS15 antibody

Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family. The protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the protein is the least conserved among small subunit ribosomal proteins.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.

General readings

Zhang, Z. et al., Genomics 81(5): 468-480 (2003)
Kenmochi, N. et al., Genomics 77 (1-2): 65-70 (2001)
Suzuki, T. et al., J. Biol. Chem. 276(35): 33181-33195 (2001)
Cavdar Koc, E. et al., J. Biol. Chem. 276(22): 19363-19374 (2001)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MRPS15 antibody

  Rabbit Polyclonal Anti-MRPS15 Antibody  
WB Suggested Anti-MRPS15 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: 721_B cell lysateMRPS15 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MRPS15 antibody

  Rabbit Polyclonal Anti-MRPS15 Antibody - middle region  
MRPS15 Rabbit IgG Purified Bov, GP, Hu, Ms, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

MRPS15 antibody

  Rabbit Polyclonal Anti-MRPS15 Antibody  
WB Suggested Anti-MRPS15 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MRPS15 antibody

  Rabbit Polyclonal Anti-MRPS15 Antibody  
WB Suggested Anti-MRPS15 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysate; MRPS15 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MRPS15 antibody

  MRPS15 mouse monoclonal antibody, clone OTI6D2 (formerly 6D2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS15 (RC207694, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS15.(1:2000) Mouse IgG2b OTI6D2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MRPS15 antibody

  MRPS15 mouse monoclonal antibody, clone OTI5F5 (formerly 5F5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS15 (RC207694, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS15.(1:2000) Mouse IgG1 OTI5F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MRPS15 (Center) antibody

Western blot analysis in A549 cell line lysates (35ug/lane) using MRPS15 antibody Cat.-No. AP52752PU-N. This demonstrates this antibody detected the MRPS15 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MRPS15 antibody

Human Lung; MRPS15 antibody - N-terminal region (ARP33299_P050) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified Bov, Hu, Ms P, WB
50 µg / €350.00
  AVIVA Systems Biology

MRPS15 antibody

Human Thymus; WB Suggested Anti-MRPS15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Thymus; MRPS15 antibody - N-terminal region (ARP39659_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

MRPS15 antibody

Human 721_B; WB Suggested Anti-MRPS15 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. MRPS15 is supported by BioGPS gene expression data to be expressed in 721_B.; MRPS15 antibody - C-terminal region (ARP39660_T100) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

MRPS15 antibody

Human Liver; WB Suggested Anti-MRPS15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; MRPS15 antibody - middle region (ARP57897_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

MRPS15 - human gene knockout kit via CRISPR

MRPS15 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MRPS15 - human gene knockout kit via CRISPR

MRPS15 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MRPS15 - human gene knockout kit via CRISPR

MRPS15 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MRPS15 - human gene knockout kit via CRISPR

MRPS15 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mrps15 - mouse gene knockout kit via CRISPR

Mrps15 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mrps15 - mouse gene knockout kit via CRISPR

Mrps15 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mrps15 - mouse gene knockout kit via CRISPR

Mrps15 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mrps15 - mouse gene knockout kit via CRISPR

Mrps15 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MRPS15 (full length, N-term HIS tag)

  Recombinant protein of human mitochondrial ribosomal protein S15 (MRPS15), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
MRPS15 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

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