MST4 antibody

Principal name

MST4 antibody

Alternative names for MST4 antibody

MASK, Serine/threonine-protein kinase MST4, STE20-like protein kinase 4, Mst3 and SOK1-related kinase, RP6-213H19.1

SwissProt ID

Q99JT2 (Mouse), Q9P289 (Human)

Gene ID

51765 (MST4)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of MST4 antibody

Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage. MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family. MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST. Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade.

General readings

Dan I, Ong SE, Watanabe NM, Blagoev B, Nielsen MM, Kajikawa E, Kristiansen TZ, Mann M, Pandey A.
Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties.
J Biol Chem. 2002 Feb 22;277(8):5929-39.
PMID: 11741893

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MST4 antibody

Immunofluorescence of monoclonal antibody to RP6-213H19.1 on HeLa cell. [antibody concentration 20 ug/ml] Mouse IgG2a 7H4 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MST4 (C-term) antibody

TA302773 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MST4 antibody

Sample (30 ug of whole cell lysate). A: IMR32 10% SDS PAGE. TA308369 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MST4 antibody

Sample (30 ug of whole cell lysate). A: Raji. 10% SDS PAGE. TA308370 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MST4 antibody

MST4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MST4 antibody

Western Blot analysis of RP6-213H19.1 expression in transfected 293T cell line (H00051765-T01) by RP6-213H19.1 MaxPab polyclonal antibody.

Lane 1: RP6-213H19.1 transfected lysate(45.76 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MST4 antibody

Western Blot analysis of RP6-213H19.1 expression in transfected 293T cell line (H00051765-T01) by RP6-213H19.1 MaxPab polyclonal antibody.

Lane 1: RP6-213H19.1 transfected lysate(45.76 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MST4 antibody

Immunoprecipitation of RP6-213H19.1 transfected lysate using anti-RP6-213H19.1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RP6-213H19.1 MaxPab mouse polyclonal antibody (B01) (H00051765-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

MST4 antibody

Western Blot analysis of RP6-213H19.1 expression in transfected 293T cell line (H00051765-T02) by RP6-213H19.1 MaxPab polyclonal antibody.

Lane 1: RP6-213H19.1 transfected lysate(46.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MST4 antibody

Immunofluorescence of monoclonal antibody to RP6-213H19.1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2b 2G6 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MST4 antibody

Immunoperoxidase of monoclonal antibody to RP6-213H19.1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml] Mouse IgG1 3G5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MST4 antibody

RP6-213H19.1 polyclonal antibody ( Cat # PAB6133 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MST4 antibody

NB 100-1004 staining (0.5µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MST4 antibody

Western Blot and Immunoprecipitation: MST4 Antibody [NB100-1583] - Detection of Human MST4(MASK) on HeLa whole cell lysate using NB100-1583. Immunoprecipitated MST4(MASK) was blotted using rabbit anti-MST4(MASK) antibody NB100-1584. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

MST4 antibody

Western Blot and Immunoprecipitation: MST4 Antibody [NB100-1584] - Detection of Human MST4(MASK) on HeLa whole cell lysate using NB100-1584. MST4(MASK) was immunoprecipitated using rabbit anti-MST4(MASK) antibody NB100-1583. Rabbit Aff - Purified Hu WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MST4 (transcript variant 1)

MST4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MST4

MST4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MST4

MST4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

MST4

MST4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MST4

MST4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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