MTHFD1 antibody
Principal name
MTHFD1 antibody
Alternative names for MTHFD1 antibody
MTHFC, MTHFD, C-1-tetrahydrofolate synthase, cytoplasmic, C1-THF synthase
Ncbi ID
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)
Background of MTHFD1 antibody
- Kit Component:
- - KN200297G1, MTHFD1 gRNA vector 1 in pCas-Guide vector
- KN200297G2, MTHFD1 gRNA vector 2 in pCas-Guide vector
- KN200297D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Dorszewska J, Florczak J, Rozycka A, Kempisty B, Jaroszewska-Kolecka J, Chojnacka K, Trzeciak WH, Kozubski W.
Oxidative DNA damage and level of thiols as related to polymorphisms of MTHFR, MTR, MTHFD1 in Alzheimer's and Parkinson's diseases.
Acta Neurobiol Exp (Wars). 2007;67(2):113-29.
PMID: 17691219
Immunofluorescence of purified MaxPab antibody to MTHFD1 on HeLa cell. [antibody concentration 10 ug/ml]
MTHFD1 MaxPab polyclonal antibody. Western Blot analysis of MTHFD1 expression in human placenta.
Western Blot analysis of MTHFD1 expression in transfected 293T cell line (H00004522-T01) by MTHFD1 MaxPab polyclonal antibody.Lane1:MTHFD1 transfected lysate(102.85 KDa).Lane2:Non-transfected lysate.
MTHFD1 MaxPab polyclonal antibody. Western Blot analysis of MTHFD1 expression in HeLa.
MTHFD1 MaxPab rabbit polyclonal antibody. Western Blot analysis of MTHFD1 expression in mouse liver.
MTHFD1 MaxPab rabbit polyclonal antibody. Western Blot analysis of MTHFD1 expression in human liver.
Western Blot analysis of MTHFD1 expression in transfected 293T cell line (H00004522-T02) by MTHFD1 MaxPab polyclonal antibody.Lane 1: MTHFD1 transfected lysate(101.60 KDa).Lane 2: Non-transfected lysate.
MTHFD1 polyclonal antibody ( Cat # PAB11432 ) ( 1 µg/ml ) staining of human substantia nigra lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry Image: Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot Image: Antibody (1 µg/ml) staining of Human Substantia Nigra lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Liver; Liver; MTHFD1 antibody - N-terminal region (ARP46146_P050) in Human Liver cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-MTHFD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate. MTHFD1 is supported by BioGPS gene expression data to be expressed in HepG2.; MTHFD1 antibody - N-terminal region (ARP46146_P050) in Human HepG2 cells using Western Blot
Human Liver; Liver; MTHFD1 antibody - middle region (ARP46147_P050) in Human Liver cells using Immunohistochemistry
Human OVCAR-3; WB Suggested Anti-MTHFD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: OVCAR-3 cell lysate; MTHFD1 antibody - middle region (ARP46147_P050) in Human OVCAR-3 cells using Western Blot
TA305850 (1ug/ml) staining of Human Substantia Nigra lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-MTHFD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: HepG2 cell lysate.MTHFD1 is supported by BioGPS gene expression data to be expressed in HepG2
Liver
WB Suggested Anti-MTHFD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: OVCAR-3 cell lysate
Liver
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA305850 | MTHFD1 antibody |
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| Goat Anti-MTHFD1 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA339293 | MTHFD1 antibody |
||||||
| Rabbit Polyclonal Anti-MTHFD1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze | P, WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA339294 | MTHFD1 antibody |
||||||
| Rabbit Polyclonal Anti-MTHFD1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze | P, WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP23100PU-N | MTHFD1 (137-150) antibody |
||||||
 |
Goat | Aff - Purified | Bt, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Rb | E, P, WB |
50 µg /
€380.00
|
||
| Acris Antibodies GmbH | |||||||
| +1 additional image |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN200297 | MTHFD1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200297BN | MTHFD1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200297LP | MTHFD1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN200297RB | MTHFD1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300297 | MTHFD1 |
||||
| Recombinant protein of human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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Lysates
| Catalog No. | ||
|---|---|---|
| LY416959 | MTHFD1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
