MTHFS antibody

Principal name

MTHFS antibody

Alternative names for MTHFS antibody

Methenyl-THF synthetase, 5-formyltetrahydrofolate cyclo-ligase, 5,10-methenyl-tetrahydrofolate synthetase

SwissProt ID

P49914 (Human), P80405 (Rabit), Q9D110 (Mouse)

Gene ID

10588 (MTHFS)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MTHFS antibody

MTHFS, also known as 5-formyltetrahydrofolate cyclo-ligase, is a cytosolic protein involved in the formate metabolic process. MTHFS, with a magnesium cofactor, catalyzes the ATP-dependent reaction that reduces 5-formyltetrahydrofolate (5-MTHF) to 5,10-methenyltetrahydrofolate(MTHF). MTHF is the substrate used by MTHFR (methylenetetrahydrofolate reductase) to generate 5-MTHF. MTHF is also a coenzyme used in thymidine biosynthesis by thymidylate synthase (FAD). Recombinant human MTHFS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Anguera MC, Stover PJ.
Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein.
Arch Biochem Biophys. 2006 Nov 15;455(2):175-87. Epub 2006 Oct 4.
PMID: 17055997

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MTHFS antibody

TA305750 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide.  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MTHFS antibody

WB Suggested Anti-MTHFS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MTHFS antibody

WB Suggested Anti-MTHFS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MTHFS antibody

MTHFS Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MTHFS antibody

MTHFS monoclonal antibody (M01), clone 2C12 Western Blot analysis of MTHFS expression in HeLa ( Cat # L013V1 ). Mouse IgG1 2C12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MTHFS antibody

MTHFS polyclonal antibody ( Cat # PAB11556 ) ( 0.5 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) with ( B ) and without ( A ) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MTHFS antibody

Transfected 293T; WB Suggested Anti-MTHFS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; MTHFS antibody - middle region (ARP52185_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MTHFS antibody

Transfected 293T; WB Suggested Anti-MTHFS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; MTHFS antibody - middle region (ARP52186_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MTHFS antibody

Western blot: MTHFS Antibody [NBP1-20947]-(0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MTHFS antibody

Western Blot: MTHFS Antibody [NBP1-56698] - This Anti-MTHFS antibody was used in Western Blot of Transfected 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

MTHFS antibody

Immunohistochemistry-Paraffin: MTHFS Antibody [NBP1-83161] - Immunohistochemical staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MTHFS

MTHFS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MTHFS (1-203, His-tag)

MTHFS Human Purified > 95 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

MTHFS (1-203, His-tag)

MTHFS Human Purified > 95 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

MTHFS

MTHFS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MTHFS

MTHFS Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

MTHFS

MTHFS Human
0.1 mg / €380.00
  Abnova Taiwan Corp.

MTHFS

MTHFS Human Purified
0.1 mg / €380.00
  Novus Biologicals Inc.

MTHFS

MTHFS Human Purified
0.5 mg / €870.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

MTHFS overexpression lysate

MTHFS overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

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