mTOR / FRAP1 antibody

Principal name

mTOR / FRAP1 antibody

Alternative names for mTOR / FRAP1 antibody

Mammalian target of rapamycin, TOR, FRAP, FRAP2, RAPT1, Serine/threonine-protein kinase mTOR, FKBP12-rapamycin complex-associated protein, FK506-binding protein 12-rapamycin complex-associated protein 1, Rapamycin target protein

SwissProt ID

P42345 (Human), P42346 (Rat), Q06RG6, Q9JLN9 (Mouse)

Gene ID

2475 (MTOR), 56717 (Mtor)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Ze (Zebrafish), Bov (Bovine), Chk (Chicken), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP)

Background of mTOR / FRAP1 antibody

Rapamycin and analogs are promising anticancer agents currently under clinical trials. When rapamycin complexed with FKBP12, rapamycin binds to and inhibits the target of rapamycin (TOR) protein, a 289-kDa phosphatidylinositide 3-kinase–related kinase (PIKK). The mammalian Target Of Rapamycin (mTOR) is a nutrient-sensing protein kinase that regulates numerous cellular processes including cell cycle progression, DNA recombination/DNA damage detection and chondrocyte differentiation along the bone growth in several species (1). The mTOR is composed of 20 in tandem repeats-HEAT which are implicated in protein–protein interactions. The mTOR protein consists of several functional domains including a carboxy terminal FAT domain, FBR domain, a kinase domain and a FATC domain. FATC domain is essential for mTOR activity and site directed mutagenesis of a single amino acid in the FATC domain results in complete loss of mTOR activity. MTOR undergoes auto phosphorylation via its intrinsic serine/threonine kinase activity and upon activation it regulates protein synthesis through phosphorylation and activation of repressor of mRNA translation 4E-BP1 and via phosphorylation and activation of S6 kinase. A regulatory associated protein of TOR (RAPTOR), PI3 kinase and ATK kinase are all known positive regulators of mTOR.
mTOR has significant homology to S. cerevisiae protein TOR1, in rat mTOR is a 289 kDa protein which has been shown to interact with immunophilin FKBP12 in a rapamycin-dependent manner. The Rapamycin-FKBP12 complex is known to inhibit progression through G1 cell cycle stage by interfering with mitogenic signaling pathways. Mammalian target of rapamycin (mTOR) forms two complexes, mTORC1 and mTORC2, that play central roles in cell growth and functions. Only mTORC1 is directly inhibited by the immunosuppressive drug rapamycin. A large portion of mTOR is localized to the endoplasmic reticulum and Golgi in many common cells (1). The mTOR targeting important for its function and the targeting is directed by HEAT repeats and the two intervening interunit spaces (2).

General readings

1. Xie L  et al. The frontal cortex IGF system is down regulated in the term, intrauterine growth restricted fetal baboon. Growth Horm IGF Res 23:187-92 (2013). Baboon .PubMed: 23911858
2. Cooper A  et al. Ewing tumors that do not overexpress BMI-1 are a distinct molecular subclass with variant biology: a report from the Children's Oncology Group. Clin Cancer Res17:56-66 (2011). IHC-P ; Human .PubMed: 21047978
3. Gulhati P  et al. Targeted inhibition of mammalian target of rapamycin signaling inhibits tumorigenesis of colorectal cancer. Clin Cancer Res 15:7207-16 (2009). IHC ; Human .PubMed: 19934294

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

mTOR / FRAP1 antibody

Immunohistochemistry (IHC) analysis of mTOR antibody (Cat.-No AP06628PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

mTOR / FRAP1 antibody

Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using mTOR Antibody . Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

mTOR / FRAP1 antibody

Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using mTOR Antibody . Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

mTOR / FRAP1 antibody

  Rabbit Polyclonal Antibody against FRAP1 (S2481)  
Western blot analysis of FRAP1 (arrow) using rabbit polyclonal FRAP1 Antibody (S2481) (Cat.#TA302216). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit Polyclonal TOR Antibody  
Western blot analysis of TOR in L1210 cell lysate with TOR antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit anti-MTOR polyclonal antibody  
Western blot analysis of extracts from 293 cells using mTOR antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Anti-MTOR Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using MTOR antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Anti-MTOR Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon cancer and brain tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using MTOR antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit Polyclonal mTOR Antibody  
Western blot analysis of mTOR expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit Polyclonal mTOR (Ser2448) Antibody (Phospho-specific)  
Western blot analysis of mTOR phosphorylation expression in EGF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit Polyclonal mTOR (Ser2481) Antibody (Phospho-specific)  
Western blot analysis of mTOR phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit Polyclonal mTOR (Thr2446) Antibody (Phospho-specific)  
Western blot analysis of mTOR phosphorylation expression in Insulin treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit anti-MTOR Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MTOR antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Phospho- MTOR- S2448 Rabbit Polyclonal Antibody  
mTOR / FRAP1 Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit Polyclonal anti-mTOR Phospho (Ser2481) Antibody  
Myc-mTOR transfected 293 cells were starved for 30 hours, then treated without (lane 1) or with (lane 2) 20% FBS for 30 minutes. The cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with Poly6517 anti-mTOR pSer2481 antibody. Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection. Rabbit Poly6517 Purified Hu WB
50 µl / €280.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Mouse Monoclonal anti-mTOR Antibody  
Total cell lysate from 293T cells (lane 1), Jurkat cells (lane 2), Hela cells (lane 3), and NIH-3T3 cells (lane 4) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-mTOR (clone 6H9B10) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse 6H9B10 Purified Hu, Ms ICC/IF, WB
0.1 mg / €248.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit Polyclonal anti-mTOR Antibody  
Human Breast Carcinoma stained with anti-mTOR antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  Rabbit Polyclonal mTOR Antibody  
Immunoprecipitation: mTOR Antibody - Several commercially available antibodies which were generated using short peptides failed to isolate the FRAP1 protein complexes. SDI's FRAP1(mTOR) antibody, 2813.00.02, was generated using in vivo expressed immunogen of 100 amino acids. It was used to pull down protein complexes through immunoprecipitation. It demonstrated that Rictor and Raptor formed the distinct complexes with FRAP (mTOR). The immunoprecipitation conditions were described in Sarbassov et al (Phosphorylation and regulation of Akt/PKB by the Rictor-mTOR complex, Science 307:1098-1101, 2005, supporting online material, material and methods). FRAP1 was detected in Western blot using FRAP1 antibody at 1:4000 dilution. The experiments were performed using MDA MB-435 human cancer cell line and NF2 was used as control. Rabbit IgG Purified Hu E, IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

mTOR / FRAP1 antibody

  MTOR mouse monoclonal antibody, clone OTI1D5 (formerly 1D5)  
Human recombinant protein fragment corresponding to amino acids 1766-2144 of human MTOR(NP_004949) produced in E.coli. Mouse IgG1 OTI1D5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

mTOR / FRAP1 antibody

  MTOR mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)  
Human recombinant protein fragment corresponding to amino acids 1766-2144 of human MTOR(NP_004949) produced in E.coli. Mouse IgG2a OTI1G4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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