Mucolipin-1 antibody

Principal name

Mucolipin-1 antibody

Alternative names for Mucolipin-1 antibody

MCOLN1, Mucolipidin, MG-2, ML4, MSTP080

SwissProt ID

Q99J21 (Mouse), Q9GZU1 (Human)

Gene ID

57192 (MCOLN1), 94178 (Mcoln1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of Mucolipin-1 antibody

The endolysosome system takes part in important cellular functions such as membrane trafficking, protein transport, autophagy and sigl transduction. Endosomes result from endocytosis of the plasma membrane and lysosomes (which are derived from late endosomes) cotin mainly hydrolytic enzymes and generally have a low interl pH. Like the endoplasmic reticulum (ER), endolysosomes also store Ca2+ (lumil Ca2+ concentration: 0.5 mM), and similarly to Ca2+ release from the ER, Ca2+ from endolysosomes may also play an important role in various sigling events. To date such candidates include members of the TRP super-family of ion channels and the two-pore Ca2+ channels (TPCs). TRPMLs, also termed mucolipins, are members of the TRP channels. In mammals, three TRPMLs are known to date (TRPML1-3 or MCOLN1-3). They are all localized to endolysosomes, although when over expressed in heterologous systems, TRPML3 is found on the plasma membrane. These channels are Ca2+ permeable and display inward rectifying current properties. Like all members of this family, TRPMLs have six transmembrane domains and intracellular N- and C-termini (relatively short tails compared to other members). They are characterized by an exceptiolly large extracellular (lumil) loop between transmembrane domains 1 and 2, and N-glycosylation sites are present in the first extracellular (lumil) loop. In mammals, TRPML1 is expressed in a ubiquitous manner and shows highest expression in the brain, kidney, spleen, liver and heart. TRPML2 and TRPML3 are less widely expressed. Interestingly, in mouse, two splice variants exist for TRPML2. The shorter variant is more broadly expressed and is domint over the longer variant in the thymus, spleen and kidney. TRPML3 is highly detected in the thymus, lung, kidney, spleen and eye, some epithelial cells and brain. Pathologies related to these channels include type IV mucolipidosis, a neurodegenetative disease characterized by retardation and retil degeneration caused by a loss of function mutation in the gene encoding TRPML1. In contrast, a gain of function mutation in TRPML3, in mice, causes deafness, and pigmentation defects.

General readings

LaPlante,J.M., et al., (2004) Biochem. Biophys. Res. Commun. 322 (4), 1384-1391

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Mucolipin-1 antibody

  Rabbit Polyclonal Mucolipin 1 Antibody  
Detection of Mucolipin-1 in mouse brain lysates using anti-Mucolipin-1. Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Mucolipin-1 antibody

  Rabbit Polyclonal Anti-TRPML1  
Western blot analysis of rat kidney (lanes 1 and 2) and HEK-293 cells (lanes 3 and 4) lysates: 1, 3. Anti-TRPML1 antibody, (1:200). 2, 4. Anti-TRPML1 antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Mucolipin-1 antibody

  Rabbit Polyclonal Anti-MCOLN1 Antibody  
WB Suggested Anti-MCOLN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

MCOLN1 - human gene knockout kit via CRISPR

MCOLN1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MCOLN1 - human gene knockout kit via CRISPR

MCOLN1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MCOLN1 - human gene knockout kit via CRISPR

MCOLN1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MCOLN1 - human gene knockout kit via CRISPR

MCOLN1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mcoln1 - mouse gene knockout kit via CRISPR

Mcoln1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mcoln1 - mouse gene knockout kit via CRISPR

Mcoln1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mcoln1 - mouse gene knockout kit via CRISPR

Mcoln1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mcoln1 - mouse gene knockout kit via CRISPR

Mcoln1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MCOLN1 overexpression lysate

MCOLN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Mcoln1 (GFP-tagged) - Mouse mucolipin 1 (Mcoln1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mcoln1 (GFP-tagged) - Mouse mucolipin 1 (Mcoln1), 200 uL, >10^7 TU/mL  
200 µl / €1,230.00
  OriGene Technologies, Inc.

Lenti ORF particles, Mcoln1 (Myc-DDK-tagged) - Mouse mucolipin 1 (Mcoln1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mcoln1 (Myc-DDK-tagged) - Mouse mucolipin 1 (Mcoln1), 200 uL, >10^7 TU/mL  
200 µl / €1,100.00
  OriGene Technologies, Inc.

14 Item(s)

per page
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