Mucolipin-3 antibody

Principal name

Mucolipin-3 antibody

Alternative names for Mucolipin-3 antibody

MCOLN3

SwissProt ID

Q8R4F0 (Mouse), Q8TDD5 (Human)

Gene ID

171166 (Mcoln3), 55283 (MCOLN3)

Available reactivities

Ms (Mouse), Hu (Human), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Sheep, Goat, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Mucolipin-3 antibody

The endolysosome system takes part in important cellular functions such as membrane trafficking, protein transport, autophagy and sigl transduction. Endosomes result from endocytosis of the plasma membrane and lysosomes (which are derived from late endosomes) cotin mainly hydrolytic enzymes and generally have a low interl pH. Like the endoplasmic reticulum (ER), endolysosomes also store Ca2+ (lumil Ca2+ concentration: 0.5 mM), and similarly to Ca2+ release from the ER, Ca2+ from endolysosomes may also play an important role in various sigling events. To date such candidates include members of the TRP super-family of ion channels and the two-pore Ca2+ channels (TPCs). TRPMLs, also termed mucolipins, are members of the TRP channels. In mammals, three TRPMLs are known to date (TRPML1-3 or MCOLN1-3). They are all localized to endolysosomes, although when over expressed in heterologous systems, TRPML3 is found on the plasma membrane. These channels are Ca2+ permeable and display inward rectifying current properties. Like all members of this family, TRPMLs have six transmembrane domains and intracellular N- and C-termini (relatively short tails compared to other members). They are characterized by an exceptiolly large extracellular (lumil) loop between transmembrane domains 1 and 2, and N-glycosylation sites are present in the first extracellular (lumil) loop. In mammals, TRPML1 is expressed in a ubiquitous manner and shows highest expression in the brain, kidney, spleen, liver and heart. TRPML2 and TRPML3 are less widely expressed. Interestingly, in mouse, two splice variants exist for TRPML2. The shorter variant is more broadly expressed and is domint over the longer variant in the thymus, spleen and kidney. TRPML3 is highly detected in the thymus, lung, kidney, spleen and eye, some epithelial cells and brain. Pathologies related to these channels include type IV mucolipidosis, a neurodegenetative disease characterized by retardation and retil degeneration caused by a loss of function mutation in the gene encoding TRPML1. In contrast, a gain of function mutation in TRPML3, in mice, causes deafness, and pigmentation defects.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Mucolipin-3 antibody

Western blot analysis of rat brain (lanes 1 and 4), rat kidney (lanes 2 and 5) and rat pancreas (lanes 3 and 6): 1, 2, 3. Anti-TRPML3 antibody, (1:200). 4, 5, 6. Anti-TRPML3 antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Mucolipin-3 antibody

WB Suggested Anti-MCOLN3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Mucolipin-3 antibody

WB Suggested Anti-MCOLN3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Mucolipin-3 antibody

Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe. Mouse IgG2a N268/19 Supernatant Hu, Ms, Rt ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

Mucolipin-3 antibody

Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe. Mouse IgG2a N268/19 Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

Mucolipin-3 antibody

Mucolipin-3 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

Mucolipin-3 antibody

Human PANC1; WB Suggested Anti-MCOLN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: PANC1 cell lysate; MCOLN3 antibody - middle region (ARP35278_P050) in Human PANC1 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Mucolipin-3 antibody

Mucolipin-3 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Mucolipin-3 antibody

Mucolipin-3 Sheep Serum Hu C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Mucolipin-3 antibody

Mucolipin-3 Rabbit Serum Ms P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Mucolipin-3 antibody

Mucolipin-3 Goat Purified Hu P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Mucolipin-3 antibody

Immunohistochemistry-Paraffin: Mucolipin 3 Antibody [NBP1-83791] Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

MCOLN3 overexpression lysate

MCOLN3 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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