Muscarinic acetylcholine receptor M5 antibody

Principal name

Muscarinic acetylcholine receptor M5 antibody

Alternative names for Muscarinic acetylcholine receptor M5 antibody

CHRM5, mAChR M5, mAChR-M5

SwissProt ID

P08911 (Rat), P08912 (Human), Q920H4 (Mouse)

Gene ID

53949 (Chrm5), 1133 (CHRM5)

Available reactivities

Rt (Rat), Hu (Human), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine), Bear, Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of Muscarinic acetylcholine receptor M5 antibody

Acetylcholine is a neurotransmitter which activates two different groups of receptors: nicotinic acetylcholine receptors (ChRs) which belong to the superfamily of ligand-gated ion channels and muscarinic acetylcholine receptors (mAChR), belonging to the G-protein coupled receptor (GPCR) superfamily. There are five separate gene products that put together this GPCR subfamily, M1-M5, and like all GPCR have seven transmembrane spanning domains. Generally, M1, M3 and M5 muscarinic receptors are known to activate phospholipase C (PLC) via Gq coupling, while M2 and M4 muscarinic receptors couple to Gi/o, and therefore inhibit adenylate cyclase. This classification is however not clear cut. mAChR can activate adenylate cyclase by coupling to Gs. In addition, M2 and M4 muscarinic receptors, when overexpressed can also activate adenylate cyclase in some systems. Evidence suggests that muscarinic receptors can form homo or heterodimers at that the dimer formed can subsequently affect the downstream sigling pathways. Muscarinic receptors have been shown to regulate voltage-gated Ca2+ channels, mely Cav2.1 as well acid sensing ion channels (ASIC). Generally speaking the actions of muscarinic receptors on ion channels can be either via 2nd messengers or through their direct action on the channels once activated. Furthermore, muscarinic receptors can also promote endocytosis of ion channels (Kv1.2 for example) by recruiting tyrosine kises that phosphorylate the channel in order to termite its activity. Other diverse and important functions of muscarinic receptors include cell growth, survival and physiology. Expression of muscarinic receptors is found in neurons in the central nervous system as well as in the peripheral nervous system. In the non-nervous system, these receptors are expressed in the cardiac and smooth muscle, lung, intestine, ovary and urothelium. M5 muscarinic receptor has been the hardest receptor to study as there is no specific pharmacology targeted against the receptor and no high level of expression has been detected in tissues. However, the effects of single knockout mice were studied and it was observed that in M5 muscarinic receptor knockout mice, there is a moderate decrease in ligand binding in the lung and bladder, two organs expressing M5 muscarinic receptor. In addition, it has been proposed that M5 muscarinic receptor may be involved in interstitial cystitis, a uriry bladder disorder.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Muscarinic acetylcholine receptor M5 antibody

Western blot analysis of extracts from Jurkat cells, using CHRM5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Muscarinic acetylcholine receptor M5 antibody

Western blot analysis of rat lung (lanes 1 and 5) and brain (lanes 2 and 6) membranes, A-875 (lanes 3 and 7) and MALM-3M (lanes 4 and 8) cell lysates: 1-4. Anti-M5 Muscarinic Receptor (extracellular) antibody, (1:200). 5-8. Anti-M5 Muscarinic Receptor (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Muscarinic acetylcholine receptor M5 antibody

Western blot analysis of extracts of various cell lines, using CHRM5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Muscarinic acetylcholine receptor M5 (3rd cytopl. dom.) antibody

Anti-CHRM5 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Mky P
50 µg / €379.00
  OriGene Technologies, Inc.

Muscarinic acetylcholine receptor M5 (3rd cytopl. dom.) antibody

Anti-CHRM5 / M5 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Can, Eq, Hu, Mky P
50 µg / €379.00
  OriGene Technologies, Inc.

Muscarinic acetylcholine receptor M5 antibody

Western blot (WB) analysis of Adenosine mAChR M5 antibody (Cat.-No AP01305PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Muscarinic acetylcholine receptor M5 antibody

Western Blot analysis of CHRM5 expression in transfected 293T cell line (H00001133-T01) by CHRM5 MaxPab polyclonal antibody.

Lane 1: CHRM5 transfected lysate(60.10 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Muscarinic acetylcholine receptor M5 antibody

Immunofluorescence analysis of A549 cells, using CHRM5 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

Muscarinic acetylcholine receptor M5 antibody

Immunofluorescence analysis of A549 cells, using CHRM5 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

Muscarinic acetylcholine receptor M5 antibody

Western Blot: mAChR M5 Antibody [NBP1-00907] - Western blot (WB) analysis of Adenosine mAChR M5 (L321) antibody in extracts from Jurkat cells Rabbit Aff - Purified Hu ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Muscarinic acetylcholine receptor M5 antibody

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M5 Antibody [NBP1-87578] Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Muscarinic acetylcholine receptor M5

Muscarinic acetylcholine receptor M5 Human
10 µg / €540.00
  Abnova Taiwan Corp.

Muscarinic acetylcholine receptor M5

Muscarinic acetylcholine receptor M5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Muscarinic acetylcholine receptor M5

Muscarinic acetylcholine receptor M5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

14 Item(s)

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