MUTYH antibody

Principal name

MUTYH antibody

Alternative names for MUTYH antibody

MYH, MutY homolog, A/G-specific adenine DNA glycosylase

SwissProt ID

Q8R5G2 (Rat), Q99P21 (Mouse), Q9UIF7 (Human)

Gene ID

4595 (MUTYH), 70603 (Mutyh), 170841

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of MUTYH antibody

MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.

General readings

Slupska MM, Baikalov C, Luther WM, Chiang JH, Wei YF, Miller JH.
Cloning and sequencing a human homolog (hMYH) of the Escherichia coli mutY gene whose function is required for the repair of oxidative DNA damage.
J Bacteriol. 1996 Jul;178(13):3885-92.
PMID: 8682794

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MUTYH antibody

Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi ( Cat # H00004595-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody (M01), clone 4D10 (Cat # H00004595-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG1 4D10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MUTYH (C-term) antibody

MUTYH Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
20 µl / €50.00
  SICGEN

MUTYH (C-term) antibody

MUTYH Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €300.00
  SICGEN

MUTYH (C-term) antibody

TA302935 (0.1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MUTYH antibody

Western blot analysis of extracts from HeLa cells and HepG2 cells, using MUTYH antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MUTYH antibody

Predicted band size: 60 kDa . Positive control: Human liver cancer and stomach cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lane1: Human liver cancer tissue lysate Lane2: Human stomach cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MUTYH antibody

Predicted band size: 60 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MUTYH antibody

Western blot analysis on HeLa cell lysate using MUTYH Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MUTYH antibody

ELISA: MYH Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

MUTYH (531-546) antibody

Colon: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MUTYH antibody

Immunohistochemistry analysis of MUTYH antibody (Cat.-No AP20553PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MUTYH (Center) antibody

Confocal immunofluorescent analysis of Hela cells using MUTYH antibody Cat.-No. AP52779PU-N, followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu, Ms F, ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MUTYH antibody

Western Blot analysis of MUTYH expression in transfected 293T cell line (H00004595-T01) by MUTYH MaxPab polyclonal antibody.

Lane 1: MUTYH transfected lysate(59.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MUTYH antibody

MUTYH Mouse IgG1 1C8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MUTYH antibody

Western blot analysis of MUTYH in 20 µgs of HeLa cell lysate. Using MUTYH polyclonal antibody ( Cat # PAB0208 ) at 2 µg/ml. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MUTYH antibody

MUTYH polyclonal antibody ( Cat # PAB6606 ) ( 0.1 µg/ml ) staining of human bone marrow lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MUTYH antibody

Immunofluorescence analysis of A549 cells, using MUTYH Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

MUTYH antibody

Immunofluorescence analysis of A549 cells, using MUTYH Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

MUTYH antibody

Immunohistochemical analysis of MUTYH staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MUTYH antibody

Immunohistochemical analysis of MUTYH staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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