Myeloid leukemia factor 1 antibody

Principal name

Myeloid leukemia factor 1 antibody

Alternative names for Myeloid leukemia factor 1 antibody

MLF1

SwissProt ID

P58340 (Human), Q32KY3 (Bovin), Q9QWV4 (Mouse)

Gene ID

4291 (MLF1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Myeloid leukemia factor 1 antibody

MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis. Recombinant human MLF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Yoneda-Kato N., et al. (2005) EMBO J. 24:1739-1749.
Winteringham L.N., et al (2004) Oncogene 23: 5105-5109.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Myeloid leukemia factor 1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504764) Mouse IgG1 OTI3A3 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MLF1 mouse monoclonal antibody. (UM500100, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB138 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Myeloid leukemia factor 1 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MLF1 mouse monoclonal antibody. (UM500100, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB138 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Myeloid leukemia factor 1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504745) Mouse IgG1 OTI5D9 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504746) Mouse IgG1 OTI1B9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504765) Mouse IgG1 OTI2D6 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

Anti-MLF1 mouse monoclonal antibody (TA504808) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MLF1(RC202774). Mouse IgG2b OTI1G4 Purified Hu, Mky F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

Western blot analysis of extracts from HUVEC cells, using MLF1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Myeloid leukemia factor 1 antibody

Anti-MLF1 mouse monoclonal antibody (TA504766) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MLF1(RC202774). Mouse IgG1 OTI2H10 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MLF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504767) Mouse IgG2b OTI3F11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLF1 (RC202774, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLF1. Mouse IgG1 OTI3B8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLF1 (RC202774, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLF1. Mouse IgG1 OTI5E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLF1 (RC202774, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLF1. Mouse IgG1 OTI3E7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLF1 (RC202774, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLF1. Mouse IgG1 OTI2C10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Myeloid leukemia factor 1 (N-term) antibody

Western blot analysis of MLF1 Antibody (N-term) (AP17562PU-N) in mouse heart tissue lysates (35ug/lane). MLF1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Myeloid leukemia factor 1 antibody

Western Blot analysis of MLF1 expression in transfected 293T cell line (H00004291-T01) by MLF1 MaxPab polyclonal antibody.

Lane1:MLF1 transfected lysate(29.48 KDa).
Lane2:Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Myeloid leukemia factor 1 antibody

Myeloid leukemia factor 1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Myeloid leukemia factor 1 antibody

Myeloid leukemia factor 1 Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Myeloid leukemia factor 1 antibody

Myeloid leukemia factor 1 Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Myeloid leukemia factor 1 antibody

Human Liver; WB Suggested Anti-MLF1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; MLF1 antibody - N-terminal region (ARP53757_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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