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Myeloperoxidase antibody

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Principal name

Myeloperoxidase antibody

Alternative names for Myeloperoxidase antibody

MPO

SwissProt ID

P05164 (Human), P11247 (Mouse)

Gene ID

4353 (MPO), 17523 (Mpo)

Ncbi ID

9606, NP_000241, NM_000250, NP_000241.1, 10090, NP_034954.2, NM_010824

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bac (Bacteria), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Myeloperoxidase antibody

Myeloperoxidase (MPO) is a functionally important component of the normal human neutrophil azurophil granule of polymorphonuclear leukocytes, host defense system. MPO is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. The inflammatory component of allergic reactions can be measured by eosinophil cationic protein (ECP, specific for eosinophils) and myeloperoxidase (MPO, specific for neutrophils) measurement (1). Neutrophils, especially in acute infection or the myeloid leukemias, may shed platelet-sized particles that can readily be distinguished from true platelets because they contain neutrophil myeloperoxidase, an enzyme that is not inhibited by glutraldehyde. Traditionally Myeloperoxidase was considered as a serological marker for ulomatosis (Churg-Strauss syndrome), the main target of anti-neutrophil cytoplasm antibodies (ANCA), Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that MPO participates in the initiation and progression of cardiovascular disease. It possesses potent pro-inflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is uncertain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic grander consideration as one of the most promising cardiac markers.
Human myeloperoxidase, a 745 aa protein is produced by a gene composed of 12 exons and 11 introns.The sequence was found to contain an open reading frame, 2,235 nucleotides coding for a protein of 745 amino acids with a calculated Mr of 83,868. The heavy chain of myeloperoxidase, consisting of 467 amino acids, was located on the COOH terminus half of the protein. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2, each of which is associated with a heme-like prosthetic group. The heme environment, defined by X-ray crystallographic analyses of MPO appears to be highly conserved in four mammalian peroxidases (lactoperoxidase, thyroid, esinophil and Myeloperoxidase). MPO when heated under non-reducing, denaturing conditions, MPO is cleaved to produced 22 and a 38kDa proteins, these two species are produced via a novel autolytic cleavage at Meth409 and Pro410 (2).

General readings

Banerjee, M. et al., Toxicol. Appl. Pharmacol. 249(1): 47-54 (2010)
Shimada, M. et al., Hum. Genet. 128(4): 433-441 (2010)
Nahon, P. et al., Antioxid. Redox Signal. 15(1): 31-38 (2010)
Wang, Y. et al., J. Huazhong Univ. Sci. Technol. Med. Sci. 30(4): 437-442 (2010)
Hua, F. et al., Zhongguo Fei Ai Za Zhi 13(2): 122-127 (2010)

DP053 - Myeloperoxidase
Formalin fixed paraffin embedded human tonsil stained with Myeloperoxidase antibody. DP053
SM1475F - Myeloperoxidase
Staining of permeabilised human peripheral blood granulocytes with Mouse anti Human Myeloperoxidase Antibody -FITC (Cat.-No SM1475F)
SM1475P - Myeloperoxidase
Staining of Human bone marrow: Formalin fixed, paraffin processed tissue with Mouse anti Human Myeloperoxidase (Cat.-No. SM1475P/PT).
SM1475P - Myeloperoxidase
Staining of Human peripheral blood granulocytes with Mouse Anti Human Myeloperoxidase antibody (Cat.-No. SM1475P/PT) following membrane permeabilisation.
AP07604SU-N - Myeloperoxidase
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen usíng anti-Myeloperoxidase antibody.
PAB1205 - Myeloperoxidase
Immunoblot analysis of cell lysate. Lane 1 : Purified human MPO protein. Lane 2 : HL-60 cell lysate.
MAB0772 - Myeloperoxidase
Immunoprecipitation analysis of HL-60 cell lysate. Lane 1 : Input. Lane 2 : Precipitates. Immunoblotting with MPO monoclonal antibody, clone 1A1 ( Cat # MAB0772 ).
PAB11927 - Myeloperoxidase
Immunohistochemical staining of MPO on formalin fixed, paraffin embedded human lymph node with MPO polyclonal antibody.
PAB7992 - Myeloperoxidase
Immunohistochemical analysis of paraffin-embedded human ovary sections using MPO polyclonal antibody ( Cat # PAB7992 ) .
PAB7992 - Myeloperoxidase
Western blot analysis of rat brain tissue lysate. Using MPO polyclonal antibody ( Cat # PAB7992 ) .
PAB8858 - Myeloperoxidase
Western blot analysis of MPO with Jurkat ( Lane 1 ) and NIH/3T3 ( Lane 2 ) cell lysate.
PAB14328 - Myeloperoxidase
Staining of Myeloperoxidase ( MPO ) in acetone fixed human neutrophils. Cytospin slides of human neutrophils isolated from peripheral blood were fixed by immersion in cold acetone for 90 seconds and blocked with 10% FCS prior to incubation with MPO polyclonal antibody ( Cat # PAB14328 ). The antibody was diluted to 1 : 1000 and incubated at room temperature for 1h and visualized with FITC-labeled secondary antibody.
AM20446SU-N - Myeloperoxidase
Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20446SU-N - Myeloperoxidase
Thymus, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20446SU-N - Myeloperoxidase
Western blot analysis using anti-MPO monoclonal antibody against truncated Trx-MPO recombinant protein (Lane 1), truncated MBP-MPO (aa1-193) recombinant protein (Lane 2) and truncated MPO (aa165-745)-hIgGFc transfected CHO-K1 cell lysate (Lane 3).
AP23272PU-N - Myeloperoxidase
Immunohistochemical analysis of paraffin embedded Human tissue sections (ovary) using Myeloperoxidase antibody
AP23272PU-N - Myeloperoxidase
Western blot analysis of rat brain tissue lysis using Myeloperoxidase antibody
AP23566PU-N - Myeloperoxidase
Human Spleen, Neutrophils: Formalin-Fixed, Paraffin-Embedded (FFPE)
ARP60967_P050 - Myeloperoxidase
Human 293T; WB Suggested Anti-MPO Antibody. . Titration: 1.0 ug/ml. . Positive Control: 293T Whole Cell. MPO is supported by BioGPS gene expression data to be expressed in HEK293T.; MPO antibody - N-terminal region (ARP60967_P050) in Human 293T cells using Western Blot
AP52737PU-N - Myeloperoxidase
Western blot analysis of Myeloperoxidase Antibody (C-term) Cat.-No AP52737PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the MPO antibody detected the MPO protein (arrow).
AP52788PU-N - Myeloperoxidase
Western blot analysis in HL-60 cell line lysates (35ug/lane) using Myeloperoxidase antibody Cat.-No. AP52788PU-N (C-term). This demonstrates this antibody detected the Myeloperoxidase protein (arrow).
AP52788PU-N - Myeloperoxidase
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram) using Myeloperoxidase antibody Cat.-No. AP52788PU-N (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
AM39035FC-N - Myeloperoxidase
Staining with clone 266.6K2 (anti-MPO intracellular) monoclonal antibody is illustrated by flow cytometry analysis of normal blood cells. Direct staining was performed using 10 µl FITC-conjugated antibody with 100 µl of blood sample using a ready-to-use fixation / permeabilization solution. Cells were fixed followed by permeabilization and incubation with anti-MPO.
AP32134PU-N - Myeloperoxidase
Staining of Human Peripheral Blood Lymphocyte lysate using Myeloperoxidase antibody Cat.-No AP32134PU-N at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CEK1288 - Human Myeloperoxidase ELISA Kit
Representative Standard Curve
CEK1535 - Mouse Myeloperoxidase ELISA Kit
Representative Standard Curve
TP316029 - Myeloperoxidase
TA318973 - Myeloperoxidase
Western blot analysis of MPO with Jurkat (Lane 1, 2) and 3T3 (Lane 3) cell lysate.
TA319305 - Myeloperoxidase
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by WB.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase.
TA327707 - Myeloperoxidase
Immunohistochemistry staining of Paraffin Bone marrow tissue by Myeloperoxidase antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)
TA343091 - Myeloperoxidase
WB Suggested Anti-MPO Antibody; Titration: 1.0 ug/ml; Positive Control: 293T Whole CellMPO is supported by BioGPS gene expression data to be expressed in HEK293T
TA353972 - Myeloperoxidase
Human Tonsil stained with anti-Myeloperoxidase (MPO) antibody.
TA353972 - Myeloperoxidase
Human Spleen stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Tonsil stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Lymph Node stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Bone Marrow stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Lung stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Lung Squamous Cell Carcinoma stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Colon Adenocarcinoma stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Lung Adenocarcinoma stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Stomach Adenocarcinoma stained with anti-MPO antibody.
TA353972 - Myeloperoxidase
Human Colon stained with anti-MPO antibody.
TA353971 - Myeloperoxidase
Human Lymph Node stained with anti-Myeloperoxidase (MPO) antibody.
TA349402 - Myeloperoxidase
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MPO Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
TA349402 - Myeloperoxidase
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (MPO Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Myeloperoxidase antibody
Staining of Human bone marrow: Formalin fixed, paraffin processed tissue with Mouse anti Human Myeloperoxidase (Cat.-No. SM1475P/PT). Mouse IgG1 2C7 Purified Hu C, E, F, P, WB
0.2 mg / €520.00
  Acris Antibodies GmbH
+1 additional image

Published recently!

Myeloperoxidase antibody
Staining of Human bone marrow: Formalin fixed, paraffin processed tissue with Mouse anti Human Myeloperoxidase (Cat.-No. SM1475P/PT). Mouse IgG1 2C7 Purified Hu C, E, F, P, WB
20 µg / €240.00
  Acris Antibodies GmbH
+1 additional image

Published recently!

Myeloperoxidase antibody
Myeloperoxidase Mouse IgG1 2C7 PE Hu F
100 Tests / €480.00
  Acris Antibodies GmbH
 

Published recently!

Myeloperoxidase antibody
  Rabbit Monoclonal anti-Myeloperoxidase (MPO) Antibody  
Human Tonsil stained with anti-Myeloperoxidase (MPO) antibody. Rabbit IgG SP72 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.
+10 additional images

Highly validated antibody!

Myeloperoxidase antibody
Staining of Human Peripheral Blood Lymphocyte lysate using Myeloperoxidase antibody Cat.-No AP32134PU-N at 0.3 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH
 

Acris recommends this product for Western blotting.

Myeloperoxidase antibody
  Rabbit polyclonal anti-Myeloperoxidase (MPO) antibody  
Western blot analysis of MPO with Jurkat (Lane 1, 2) and 3T3 (Lane 3) cell lysate. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Myeloperoxidase antibody
  Rabbit polyclonal Myeloperoxidase antibody  
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by WB.  Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. Rabbit IgG Serum Hu E, WB
2 ml / €1,070.00
  OriGene Technologies, Inc.

Myeloperoxidase antibody
  Rabbit Polyclonal Myeloperoxidase Antibody  
Immunohistochemistry staining of Paraffin Bone marrow tissue by Myeloperoxidase antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Rabbit Purified Hu P
1 ml / €500.00
  OriGene Technologies, Inc.

Myeloperoxidase antibody
  Rabbit Polyclonal Anti-MPO Antibody  
WB Suggested Anti-MPO Antibody; Titration: 1.0 ug/ml; Positive Control: 293T Whole CellMPO is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Myeloperoxidase antibody
  Rabbit Polyclonal Anti-MPO Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (MPO Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

Myeloperoxidase antibody
  Mouse Monoclonal anti-MPO Antibody  
Myeloperoxidase Mouse IgG1 N9A3(1325F) Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Myeloperoxidase antibody
  Mouse Monoclonal anti-MPO Antibody  
Myeloperoxidase Mouse IgG1 N4C7(989B) Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Myeloperoxidase antibody
  Rabbit Polyclonal anti-Myeloperoxidase (MPO) Antibody  
Human Lymph Node stained with anti-Myeloperoxidase (MPO) antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Myeloperoxidase antibody
Myeloperoxidase Mouse IgG2b 4A4 Purified Hu WB
0.2 mg / €360.00
  Acris Antibodies GmbH

Myeloperoxidase antibody
Myeloperoxidase Mouse IgG2a 2A11 Purified Hu
0.2 mg / €370.00
  Acris Antibodies GmbH

Myeloperoxidase antibody
Myeloperoxidase Mouse IgG2b 59A5 Supernatant Hu P
1 ml / €460.00
  Acris Antibodies GmbH

Myeloperoxidase antibody
Myeloperoxidase Mouse IgG2b 59A5 Supernatant Hu P
0.5 ml / €310.00
  Acris Antibodies GmbH

Myeloperoxidase (1-193) antibody
Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 9B12G7 Ascites Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH
+2 additional images

Myeloperoxidase antibody
Myeloperoxidase Mouse IgG1 2D4 Biotin Ms, Rt C, E, ICC/IF
50 µg / €370.00
  Acris Antibodies GmbH

Myeloperoxidase antibody
Myeloperoxidase Mouse IgG1 2D4 FITC Ms, Rt C, E, ICC/IF
0.1 mg / €540.00
  Acris Antibodies GmbH

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