MYL1 antibody

Principal name

MYL1 antibody

Alternative names for MYL1 antibody

MLC1F/MLC3F, MLC1/MLC3, Myosin light chain A1/A2, A1 catalytic, Alkali myosin light chain 1, Myosin light chain 1/3, skeletal muscle isoform

SwissProt ID

A0JNJ5 (Bovin), P02600 (Rat), P05976 (Human), P05977 (Mouse)

Gene ID

4632 (MYL1)

Available reactivities

Bov (Bovine), Hu (Human), Por (Porcine), Rb (Rabbit), Ms (Mouse), Chk (Chicken), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of MYL1 antibody

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

General readings

DiGiovanna MP et al. Production of Polyclonal anti-Phosphopeptide Antibodies. Curr. Prot. Immunol. 11:6.1-6.19 (1996).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYL1 antibody

Immunoperoxidase of monoclonal antibody to MYL1 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml] Mouse IgG2a 2D9 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MYL1 pSer20 antibody

Immunohistochemistry-Paraffin: Fast skeletal myosin light chain 1 [p Ser20] Antibody [NB100-1687] - Immunohistochemistry. affinity purified anti-Monophosphorylated RLC Smooth and Non-Muscle Myosin pS19/20 antibody was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Rabbit Aff - Purified Hu E, IP, P, WB
50 µg / €360.00
  Novus Biologicals Inc.

MYL1 antibody

MYL1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MYL1 antibody

MYL1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYL1 (transcript variant 3f)

MYL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MYL1

MYL1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MYL1

MYL1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

MYL1

MYL1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MYL1

MYL1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MYL1 293T Cell Transient Overexpression Lysate(Denatured)

MYL1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

MYL1 overexpression lysate

MYL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

11 Item(s)

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