MYL12A antibody

Principal name

MYL12A antibody

Alternative names for MYL12A antibody

MLCB, MRLC3, RLC, MLC-2B, Myosin RLC, Myosin regulatory light chain 12A, Myosin regulatory light chain 2 nonsarcomeric, Myosin regulatory light chain MRLC3

SwissProt ID

P19105 (Human)

Gene ID

10627 (MYL12A)

Available reactivities

Av (Birds), Hu (Human), Mam (Mammalia), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)

Background of MYL12A antibody

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

General readings

Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162.

  1. Quick-Filters:
  2. Cited (1)

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYL12A pSer19/20 antibody

Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by WB. Either 13 or 20 µl of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE.  Samples were either mock-treated or CLA-treated, as indicated.  After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG  preceded color development using Amersham's substrate system. Rabbit Aff - Purified Av, Hu, Mam E, IP, P, WB
0.1 mg / €900.00
  OriGene Technologies, Inc.

MYL12A antibody

MYL12A Rabbit IgG EP1456Y Purified Hu F, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYL12A

MYL12A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MYL12A (1-171, His-tag)

MYL12A Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

MYL12A (1-171, His-tag)

MYL12A Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

MYL12A

MYL12A Human
0.1 mg / €380.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MYL12A overexpression lysate

MYL12A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

7 Item(s)

per page
  • LinkedIn