MYL6 antibody

Principal name

MYL6 antibody

Alternative names for MYL6 antibody

Myosin light polypeptide 6, LC17, MLC-3, Myosin light chain 3, Myosin light chain alkali 3, 17 kDa myosin light chain, Smooth muscle and nonmuscle myosin light chain alkali 6

SwissProt ID

P02607 (Chick), P60660 (Human), P60662 (Pig), Q60605 (Mouse), Q64119 (Rat)

Gene ID

4637 (MYL6)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Sh (Sheep), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of MYL6 antibody

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.

General readings

Fu,Z.Y., (2006) Acta Biochim. Biophys. Sin. (Shanghai) 38 (9), 625-632

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYL6 antibody

Human Skeletal Muscle; MYL6 antibody - N-terminal region (ARP57711_P050) in Human Skeletal Muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

MYL6 antibody

Western blot analysis of extracts from K562 cells, using MYL6 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYL6 (12-27) antibody

WB using anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2).The primary antibody was diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MYL6 antibody

WB Suggested Anti-MYL6 Antibody Titration: 1 ug/ml; Positive Control: HepG2 cell lysateMYL6 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MYL6 antibody

MYL6 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MYL6 antibody

MYL6 monoclonal antibody (M03), clone 1D6. Western Blot analysis of MYL6 expression in Raw 264.7 ( Cat # L024V1 ). Mouse IgG2a 1D6 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MYL6 antibody

Detection limit for recombinant GST tagged MYL6 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 1G3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MYL6 pSer19/20 antibody

Immunohistochemical staining with MYL6 (phospho S19/20) polyclonal antibody ( Cat # PAB9961 ) was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA. Rabbit Hu E, IP, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

MYL6 antibody

Human Prostate; MYL6 antibody - middle region (ARP57712_P050) in Human Prostate cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

MYL6 antibody

Western blot analysis of MYL6 expression in HeLa (A); K562 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt, Sh WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MYL6 antibody

Western blot analysis of MYL6 expression in HeLa (A); K562 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt, Sh WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MYL6 antibody

Western blot analysis of MYL6 expression in HeLa (A); K562 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt, Sh WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MYL6 antibody

Western Blot: MYL6 Antibody [NBP1-54376] - This Anti-MYL6 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYL6 (transcript variant 1)

MYL6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MYL6 (transcript variant 2)

MYL6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MYL6 (1-151, His-tag)

Recombinant human MYL6, 1-151 aa, His-tagged Human Purified > 90 % E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

MYL6 (1-151, His-tag)

Recombinant human MYL6, 1-151 aa, His-tagged Human Purified > 90 % E. coli
50 µg / €370.00
  Acris Antibodies GmbH

MYL6

MYL6 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MYL6

MYL6 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

MYL6

MYL6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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