MYL6 antibody

Principal name

MYL6 antibody

Alternative names for MYL6 antibody

Myosin light polypeptide 6, LC17, MLC-3, Myosin light chain 3, Myosin light chain alkali 3, 17 kDa myosin light chain, Smooth muscle and nonmuscle myosin light chain alkali 6

SwissProt ID

P02607 (Chick), P60660 (Human), P60662 (Pig), Q60605 (Mouse), Q64119 (Rat)

Gene ID

4637 (MYL6), 17904 (Myl6), 362816 (Myl6l)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Sh (Sheep), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of MYL6 antibody

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.

General readings

Fu,Z.Y., (2006) Acta Biochim. Biophys. Sin. (Shanghai) 38 (9), 625-632

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYL6 antibody

  Rabbit polyclonal anti-MYL6 antibody  
Western blot analysis of extracts from K562 cells, using MYL6 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYL6 (12-27) antibody

  Rabbit polyclonal anti-Myosin antibody  
WB using anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2).The primary antibody was diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MYL6 antibody

  Rabbit Polyclonal Anti-MYL6 Antibody - middle region  
WB Suggested Anti-MYL6 Antibody Titration: 1 ug/ml; Positive Control: HepG2 cell lysateMYL6 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MYL6 antibody

  Rabbit Polyclonal Anti-MYL6 Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse stomach tissue, Primary antibody: (MYL6 Antibody) at dilution 1/262.5, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

MYL6 - human gene knockout kit via CRISPR

MYL6 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MYL6 - human gene knockout kit via CRISPR

MYL6 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MYL6 - human gene knockout kit via CRISPR

MYL6 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MYL6 - human gene knockout kit via CRISPR

MYL6 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYL6 (transcript variant 1)

  Recombinant protein of human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 1  
MYL6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MYL6 (transcript variant 2)

  Recombinant protein of human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 2  
MYL6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MYL6 (1-151, His-tag)

Recombinant human MYL6, 1-151 aa, His-tagged Human Purified > 90 % E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

MYL6 (1-151, His-tag)

Recombinant human MYL6, 1-151 aa, His-tagged Human Purified > 90 % E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

MYL6 overexpression lysate

MYL6 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MYL6 overexpression lysate

MYL6 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MYL6 (mGFP-tagged) - Human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, MYL6 (mGFP-tagged) - Human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, MYL6 (mGFP-tagged) - Human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, MYL6 (mGFP-tagged) - Human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, MYL6 (Myc-DDK tagged) - Human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, MYL6 (Myc-DDK tagged) - Human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Myl6 (GFP-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (cDNA clone MGC:102200 IMAGE:6824167), complete, 200 uL, >10^7 TU/mL

Lenti ORF particles, Myl6 (GFP-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (cDNA clone MGC:102200 IMAGE:6824167), complete, 200 uL, >10^7 TU/mL  
200 µl / €790.00
  OriGene Technologies, Inc.

Lenti ORF particles, Myl6 (GFP-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (Myl6), 200 uL, >10^7 TU/mL

Lenti ORF particles, Myl6 (GFP-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (Myl6), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Myl6 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (Myl6), 200 uL, >10^7 TU/mL

Lenti ORF particles, Myl6 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (Myl6), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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