MYL9 antibody

Principal name

MYL9 antibody

Alternative names for MYL9 antibody

MLC2, MRLC1, MYRL2, Myosin RLC, MLC-2C, LC20, smooth muscle, Myosin regulatory light polypeptide 9, Myosin regulatory light chain MRLC1, Myosin regulatory light chain 2

SwissProt ID

P24844 (Human), P29269 (Pig), Q5E9E2 (Bovin), Q64122 (Rat), Q9CQ19 (Mouse)

Gene ID

10398 (MYL9), 98932 (Myl9)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Av (Birds), Mam (Mammalia), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MYL9 antibody

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

General readings

Gilles, L. et al., Blood 114(19): 4221-4232 (2009)
Higashihara, M. et al., J Smooth Muscle Res 44(1): 29-40 (2008)
Szczesna-Cordary, D. et al., J. Cell. Sci. 118 (PT 16): 3675-3683 (2005)
Webb, R.C., Adv Physiol Educ 27 (1-4): 201-206 (2003)
Deloukas, P. et al., Nature 414(6866): 865-871 (2001)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYL9 antibody

Predicted band size: 14 kDa . Positive control: Jurkat and 231 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYL9 antibody

Predicted band size: 14 kDa . Positive control: Jurkat and 231 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYL9 antibody

MYL9 Rabbit IgG Purified Av, Hu, Ms, Rt E, WB
50 µg / €410.00
  Acris Antibodies GmbH

MYL9 antibody

Heart: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MYL9 (12-27) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Colon, Smooth Muscle. Rabbit Aff - Purified Av, Hu, Mam, Ms, Rt E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MYL9 (C-term) antibody

Western blot analysis in Jurkat cell line lysates (35ug/lane) using MYL9 antibody Cat.-No. AP52791PU-N (C-term). This demonstrates this antibody detected the MYL9 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MYL9 antibody

MYL9 Mouse IgG2b 3F2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MYL9 antibody

Western blot using MYL9 polyclonal antibody ( Cat # PAB11255 ) to detect vascular myosin ( rat aorta, lane 1 ), but not cardiac myosin ( mouse heart, lane2 ).
Each lanewas loaded with 35 µg of lysate.
Arrowheads indicate the detection of both mono-phosphorylated ( upper ) and unphosphorylated ( lower ) forms of the protein.
After blocking with 5% NGS and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to1 : 600 for 2 h at room temperature.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye 800TM conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature ( 800nm channel, green ).
Molecular weight estimation was made by comparison to prestained MW markers in lane M ( 700 nm channel, red ).
IRDyeTM 800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developedby LI-COR.
IRDye is a trademark of LI-COR, Inc. Rabbit Hu, Ms, Rt E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

MYL9 pSer18 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

MYL9 pSer18 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

MYL9 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRLC2 (Ab-18) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

MYL9 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRLC2 (Ab-18) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

MYL9 antibody

Human Jurkat; WB Suggested Anti-MYL9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; MYL9 antibody - middle region (ARP48288_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

MYL9 pSer18 antibody

Western blot analysis of MYL9 (pS18) expression in LO2 EGF-treated (A); Raw264.7 EGF-treated (B); rat heart (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

MYL9 pSer18 antibody

Western blot analysis of MYL9 (pS18) expression in LO2 EGF-treated (A); Raw264.7 EGF-treated (B); rat heart (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

MYL9 pSer18 antibody

Western blot analysis of MYL9 (pS18) expression in LO2 EGF-treated (A); Raw264.7 EGF-treated (B); rat heart (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

MYL9 antibody

MYL9 Rabbit Aff - Purified Av, Hu, Mam, Ms, Rt E, IP, P, WB
50 µg / €440.00
  Novus Biologicals Inc.

MYL9 antibody

Western Blot: MYL9 Antibody [NBP1-55383] - This Anti-MYL9 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYL9 (transcript variant 2)

MYL9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

MYL9 (1-172, His-tag)

MYL9 Human Purified > 90 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

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