MYL9 antibody

Principal name

MYL9 antibody

Alternative names for MYL9 antibody

MLC2, MRLC1, MYRL2, Myosin RLC, MLC-2C, LC20, smooth muscle, Myosin regulatory light polypeptide 9, Myosin regulatory light chain MRLC1, Myosin regulatory light chain 2

SwissProt ID

P24844 (Human), P29269 (Pig), Q5E9E2 (Bovin), Q64122 (Rat), Q9CQ19 (Mouse)

Gene ID

10398 (MYL9), 98932 (Myl9), 296313

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Av (Birds), Mam (Mammalia), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MYL9 antibody

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, Mouse and Rat have almost identical sequences.

General readings

Gilles, L. et al., Blood 114(19): 4221-4232 (2009)
Higashihara, M. et al., J Smooth Muscle Res 44(1): 29-40 (2008)
Szczesna-Cordary, D. et al., J. Cell. Sci. 118 (PT 16): 3675-3683 (2005)
Webb, R.C., Adv Physiol Educ 27 (1-4): 201-206 (2003)
Deloukas, P. et al., Nature 414(6866): 865-871 (2001)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYL9 antibody

  Anti-MYL9 Rabbit Polyclonal Antibody  
Predicted band size: 14 kDa . Positive control: Jurkat and 231 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYL9 antibody

  Anti-MYL9 Rabbit Polyclonal Antibody  
Predicted band size: 14 kDa . Positive control: Jurkat and 231 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYL9 antibody

MYL9 Rabbit IgG Purified Av, Hu, Ms, Rt E, WB
50 µg / €410.00
  Acris Antibodies GmbH

MYL9 antibody

Heart: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MYL9 (12-27) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Colon, Smooth Muscle. Rabbit Aff - Purified Av, Hu, Mam, Ms, Rt E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MYL9 (C-term) antibody

Western blot analysis in Jurkat cell line lysates (35ug/lane) using MYL9 antibody Cat.-No. AP52791PU-N (C-term). This demonstrates this antibody detected the MYL9 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MYL9 pSer18 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

MYL9 pSer18 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Myosin regulatory light chain 2 (Phospho-Ser18) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

MYL9 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRLC2 (Ab-18) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

MYL9 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRLC2 (Ab-18) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

MYL9 - human gene knockout kit via CRISPR

MYL9 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Myl9 - mouse gene knockout kit via CRISPR

Myl9 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Myl9 - mouse gene knockout kit via CRISPR

Myl9 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Myl9 - mouse gene knockout kit via CRISPR

Myl9 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Myl9 - mouse gene knockout kit via CRISPR

Myl9 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYL9 (transcript variant 2)

  Recombinant protein of human myosin, light chain 9, regulatory (MYL9), transcript variant 2  
MYL9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

MYL9 (1-172, His-tag)

MYL9 Human Purified > 90 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

MYL9 (1-172, His-tag)

MYL9 Human Purified > 90 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH

MYL9 (1-172, His-tag)

MYL9 Mouse Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

MYL9 (1-172, His-tag)

MYL9 Mouse Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Items 1 to 20 of 28 total

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