MYLK antibody

Principal name

MYLK antibody

Alternative names for MYLK antibody

Myosin light chain kinase, smooth muscle, MLCK, Telokin, Kinase-related protein

SwissProt ID

O02827 (Sheep), P11799 (Chick), Q15746 (Human), Q28824 (Bovin), Q6PDN3 (Mouse)

Gene ID

4638 (MYLK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Mky (Monkey), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MYLK antibody

MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYLK antibody

(0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MYLK antibody

Western blot analysis of extracts from HepG2 cells, using MYLK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYLK antibody

Western blot analysis of MYLK expression in HepG2 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MYLK antibody

Immunohistochemical staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

MYLK (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MYLK antibody

MYLK MaxPab polyclonal antibody. Western Blot analysis of MYLK expression in human kidney. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MYLK antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MYLK and CTTN. HeLa cells were stained with anti-MYLK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MYLK antibody

MYLK Mouse IgG1 1D1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MYLK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody ( Cat # PAB3181 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MYLK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody ( Cat # PAB3182 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MYLK antibody

Western blot analysis of MLCK1 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rb, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MYLK antibody

Western blot analysis of MLCK1 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rb, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MYLK antibody

Western blot analysis of MLCK1 expression in HeLa (A); SP2/0 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rb, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MYLK antibody

ELISA: Myosin light chain kinase Antibody [32180002] Rabbit Hu E, P
50 µg / €440.00
  Novus Biologicals Inc.

MYLK antibody

Immunohistochemistry-Paraffin: Myosin Antibody [NBP1-87744] - Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYLK (transcript variant 7)

MYLK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MYLK

MYLK Human
2 µg / €300.00
  Abnova Taiwan Corp.

MYLK

MYLK Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

MYLK

MYLK Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

MYLK

MYLK Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

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