Myocilin antibody

Principal name

Myocilin antibody

Alternative names for Myocilin antibody

MYOC, GLC1A, TIGR, Trabecular meshwork-induced glucocorticoid response protein

SwissProt ID

O70624 (Mouse), Q99972 (Human), Q9R1J4 (Rat), Q9XTA3 (Bovin)

Gene ID

4653 (MYOC), 17926 (Myoc), 81523

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Mky (Monkey), Prim (Primate), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), EPair for Elisa (EPair)

Background of Myocilin antibody

Approximately 4 years ago, the first major (biochemical, molecular biologic, and biologic) insight into primary open-angle glaucoma (POAG) was the presence of mutations in the myocilin (Trabecular meshwork-induced Glucocorticoid Response (Myo/TIGR) gene were related to certain forms of juvenile onset of this disease. Since then, a great deal of work has been done to determine the possible mechanisms by which MYOC/TIGR might cause not only juvenile but also adult-onset primary open-angle glaucoma. Mutation in the myocilin gene is associated with 4% (familial form) of glaucoma cases. Glaucoma is a heterogeneous eye disease and a major cause of blindness worldwide. It has been shown that primary open angle glaucoma (POAG)-associated mutations have been found in the trabecular meshwork inducible glucocorticoid response gene (TIGR), also known as the myocilin gene (MYOC), at the GLC1A locus on chromosome 1q21-q31 (1). Mutation in the TIGR protein coding region is mutated in POAG patients range from 3-4%. Myocilin is found as myocilin-Myocilin complex in the human aqueous humor and these interactions occur between amino acid 117-166 lying with in the leucine zipper motifs. Site directed mutations of the amino acid leucine in the leucine zipper results in disruption of these interactions and mutation (2).
The human clone encoding for Myocilin protein of molecular weight of 55 kDa has been isolated and characterized (4). Myocilin, which resembles Myosin, is expressed preferentially in the ciliary rootlets and the basal body of the connecting cilium of the photoreceptor cells. The deduced amino acid sequence of the human myocilin showed significant homologies with non-muscle myosin of dictyostilium discoideum in the N-terminal region and also with olfactomedin of bullfrog in the C-terminal region. Myocilin has a leucine zipper like motif that interacts with other cytoskeletal proteins.
The TIGR/myocilin protein is a 55 kDa protein expressed in RPEs.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Myocilin antibody

Human Vessel: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 4F8 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Myocilin antibody

  Anti-MYOC Rabbit Polyclonal Antibody  
Predicted band size: 57 kDa . Positive control: Heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Myocilin antibody

  Rabbit Polyclonal Anti-MYOC Antibody  
Host: Rabbit; Target Name: MYOC; Sample Tissue: ACHN whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified GP, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Myocilin (69-85) antibody

Formalin-Fixed Paraffin-Embedded Human Eye, retina tissue using AP08544PU-N at 5 µg/ml. Rabbit IgG Purified Hu, Mky, Prim E, P
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

MYOC - human gene knockout kit via CRISPR

MYOC - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MYOC - human gene knockout kit via CRISPR

MYOC - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MYOC - human gene knockout kit via CRISPR

MYOC - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MYOC - human gene knockout kit via CRISPR

MYOC - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Myoc - mouse gene knockout kit via CRISPR

Myoc - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Myoc - mouse gene knockout kit via CRISPR

Myoc - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Myoc - mouse gene knockout kit via CRISPR

Myoc - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Myoc - mouse gene knockout kit via CRISPR

Myoc - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Myocilin

  Recombinant protein of human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC)  
Myocilin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MYOC (mGFP-tagged) - Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC), 200 uL, >10^7 TU/mL

Lenti ORF particles, MYOC (mGFP-tagged) - Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Myoc (GFP-tagged) - Mouse myocilin (Myoc), 200 uL, >10^7 TU/mL

Lenti ORF particles, Myoc (GFP-tagged) - Mouse myocilin (Myoc), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.

Lenti ORF particles, Myoc (Myc-DDK-tagged) - Mouse myocilin (Myoc), 200 uL, >10^7 TU/mL

Lenti ORF particles, Myoc (Myc-DDK-tagged) - Mouse myocilin (Myoc), 200 uL, >10^7 TU/mL  
200 µl / €990.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Myoc (GFP-tagged ORF) - Rat myocilin (Myoc), 200 uL, >10^7 TU/mL

Lenti ORF particles, Myoc (GFP-tagged ORF) - Rat myocilin (Myoc), 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.

Lenti ORF particles, Myoc (Myc-DDK-tagged ORF) - Rat myocilin (Myoc), 200 uL, >10^7 TU/mL

Lenti ORF particles, Myoc (Myc-DDK-tagged ORF) - Rat myocilin (Myoc), 200 uL, >10^7 TU/mL  
200 µl / €1,004.00
  OriGene Technologies, Inc.

18 Item(s)

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