MYOD1 antibody

Principal name

MYOD1 antibody

Alternative names for MYOD1 antibody

MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3, BHLHC1, Class C basic helix-loop-helix protein 1

SwissProt ID

P10085 (Mouse), P15172 (Human), P16075 (Chick), P49811 (Pig), Q02346 (Rat), Q7YS82 (Bovin)

Gene ID

4654 (MYOD1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Fe (Feline)

Available hosts

Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of MYOD1 antibody

MyoD is a myogenic determination basic helix loop helix protein that comprises 318 amino acids. The human homolog is known as Myf 3. This transcription factor is expressed in myogenic cells and developing myogenic tissues. MyoD belongs to the family of myogenic transcription factors which include myf 5, myogenin (myf4) and MRF4 (myf6 or herculin) that are involved in programming various stages of skeletal muscle development. In this regard members of the MyoD1 family, in particular MyoD, have been useful as markers for identifying cells of the myogenic lineage for example rhabdomyosarcoma tumor cells (skeletal muscle tumor cells) and other tumors that also comprise a myogenic component. It has been shown that forced expression of MyoD (or related members) in non-muscle cells either converts the cells to myogenic cells or transcriptionally activates a set of otherwise silent muscle-specific genes in these cells. Members of the MyoD family play distinct roles during muscle development; MyoD and Myf-5 act early during myogenesis. Inactivation of MyoD results in the up-regulation of myf5 and similarly inactivation of myf5 results in the up-regulation of MyoD. Myogenin and MRF4 act later in myogenesis and are associated with muscle maturation. 
Members of the MyoD1 family, in particular MyoD, have been useful as markers for identifying cells of the myogenic lineage. This has been particularly useful for distinguishing poorly differentiated rhabdomyosarcoma (tumor of the skeletal muscle lineage) from other non muscle tumors. Thus, antibodies to MyoD have been particularly useful for distinguishing rhabdomyosarcomas from other small round cell tumors or poorly differentiated tumors that have with minimum signs of lineage specific differentiation.

General readings

Xynos, A. et al., Stem Cells 28(5): 965-973 (2010)
Stuelsatz, P. et al., J. Biol. Chem. 285(17): 12670-12683 (2010)
Hiraoka, S. et al., Hum. Pathol. 41(1): 38-47 (2010)
Yerges, L.M. et al., J. Bone Miner. Res. 24(12): 2039-2049 (2009)
Yang, Z. et al., Genes Dev. 23(6): 694-707 (2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33304PU (Clone 5.8A) Mouse IgG1 5.8A Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33304PU (Clone 5.8A) Mouse IgG1 5.8A Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33304PU (Clone 5.8A) Mouse IgG1 5.8A Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33305PU (Clone MYD712). Mouse IgG1 MYD712 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33305PU (Clone MYD712). Mouse IgG1 MYD712 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33305PU (Clone MYD712). Mouse IgG1 MYD712 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33306PU (Clone 5.8A+MYD712) Mouse IgG1 5.8A + MYD712 Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33306PU (Clone 5.8A+MYD712) Mouse IgG1 5.8A + MYD712 Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-Paraffin Rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM33306PU (Clone 5.8A+MYD712) Mouse IgG1 5.8A + MYD712 Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-paraffin rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM50144PU (Clone SPM427). Mouse IgG1 SPM427 Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-paraffin rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM50144PU (Clone SPM427). Mouse IgG1 SPM427 Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

MYOD1 antibody

Formalin-paraffin rhabdomyosarcoma stained with MyoD1 Antibody Cat.-No AM50144PU (Clone SPM427). Mouse IgG1 SPM427 Purified Chk, Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

MYOD1 antibody

Western Blot: MyoD1 Antibody - WB detection of MYOD1 in RH30 cells. Rabbit Purified Hu, Ms
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYOD1 antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human ovarian and lung cancer tissue. Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded Human ovarian and lung cancer tissue using MYOD1 antibody at dilution 1/120, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYOD1 (Center) antibody

MyoD1 Antibody (Center) (Cat. #TA324498) western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow). Rabbit Ig Aff - Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MYOD1 antibody

WB Suggested Anti-MYOD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Spleen Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MYOD1 antibody

Western Blot: MyoD1 Antibody (5.8A) [TA336376] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/ml anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but w Mouse IgG1 5.8A Purified Fe, Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MYOD1 antibody

Western blot analysis using MYOD1 antibody Cat.-No AM06516SU-N against HEK293 (1) and MYOD1(AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 1C8 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

MYOD1 antibody

Staining of Rh30 rhabdomyosarcoma cells at using MyoD Antibody Cat.-No AM32709PU-N (Clone BC1-C2H8) at 5 µg/ml is shown. 
Secondary Antibody: FITC conjugated anti Mouse Ig. Mouse IgG1 BC1-C2H8 Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

MYOD1 pSer200 antibody

Figure 1. Western blot analysis of extract from HeLa cells, untreated or treated with vanadate (1 mg/ml, 30 min), using MyoD (phospho- Ser200) antibody AP02375PU. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

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