Myogenin antibody

Principal name

Myogenin antibody

Alternative names for Myogenin antibody

MYOG, MYF4, Myogenic factor 4, BHLHC3, Class C basic helix-loop-helix protein 3

SwissProt ID

P12979 (Mouse), P15173 (Human), P17920 (Chick), P20428 (Rat), P49812 (Pig), Q7YS81 (Bovin)

Gene ID

4656 (MYOG), 29148 (Myog), 17928

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Electrophoretic Mobility Shift Assay (EMSA), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Myogenin antibody

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.

General readings

Gao, X. et al., J. Cell. Biochem. 110(1): 162-170 (2010)
Yerges, L.M. et al., J. Bone Miner. Res. 24(12): 2039-2049 (2009)
Ramamoorthy, S. et al., Am. J. Physiol. Endocrinol. Metab. 297 (2): E392-E401 (2009)
Nanni, P. et al., Mol. Cancer Ther. 8(4): 754-761 (2009)
Gong, C. et al., Genes Dev. 23(1): 54-66 (2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Myogenin antibody

Formalin-Fixed, Paraffin-Embedded Human rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM32825PU (Clone MGN185) at 1/200 dilution using peroxidase-conjugate and DAB chromogen. Mouse IgG1 MGN185 Purified Fe, Hu, Ms, Por, Rt C, E, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin-Fixed, Paraffin-Embedded Human rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM32825PU (Clone MGN185) at 1/200 dilution using peroxidase-conjugate and DAB chromogen. Mouse IgG1 MGN185 Purified Fe, Hu, Ms, Por, Rt C, E, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin-Fixed, Paraffin-Embedded Human rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM32825PU (Clone MGN185) at 1/200 dilution using peroxidase-conjugate and DAB chromogen. Mouse IgG1 MGN185 Purified Fe, Hu, Ms, Por, Rt C, E, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin-Paraffin Rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM33347PU (Clone F5D). Mouse IgG1 F5D Purified Can, Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin-Paraffin Rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM33347PU (Clone F5D). Mouse IgG1 F5D Purified Can, Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin-Paraffin Rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM33347PU (Clone F5D). Mouse IgG1 F5D Purified Can, Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin Paraffin rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM50145PU (Clone SPM144). Mouse IgG1 SPM144 Purified Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin Paraffin rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM50145PU (Clone SPM144). Mouse IgG1 SPM144 Purified Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin Paraffin rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM50145PU (Clone SPM144). Mouse IgG1 SPM144 Purified Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin-Fixed, Paraffin-Embedded Human rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM50302PU (Clone MGN185+F5D). Mouse IgG1 MGN185 + F5D Purified Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin-Fixed, Paraffin-Embedded Human rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM50302PU (Clone MGN185+F5D). Mouse IgG1 MGN185 + F5D Purified Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Myogenin antibody

Formalin-Fixed, Paraffin-Embedded Human rhabdomyosarcoma stained with Myogenin Antibody Cat.-No AM50302PU (Clone MGN185+F5D). Mouse IgG1 MGN185 + F5D Purified Fe, Hu, Ms, Por, Rt C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Myogenin (C-term) antibody

(2ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Myogenin antibody

Immunohistochemistry staining of Paraffin Rhabdomyosarcoma tissue by Myogenin antibody (dilution: 1:25 - 1:100; visualization of staining: Nuclear) Mouse IgG1 F5D Purified Hu P
1 ml / €420.00
  OriGene Technologies, Inc.

Myogenin antibody

Western Blot: Myogenin Antibody (5FD) [TA336375] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line (Rh30). Negative cell lines were PFSK-1, EB2 Mouse IgG1 5FD Purified Hu, Ms, Rt C, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Myogenin antibody

Formalin-Fixed, Paraffin-Embedded Human rhabdomyosarcoma stained with Myogenin antibody Cat.-No. AM00317SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of tumor cells. Mouse IgG1 F5D Supernatant Fe, Hu, Ms, Rt ICC/IF, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

Myogenin antibody

Figure 1. Immunohistochemical staining using myogenin antibody (AM05309PU-N) on formalin fixed, paraffin embedded human rhabdomyosarcoma. Mouse IgG1 F12B Purified Hu, Ms, Rt E, EMSA, ICC/IF, IP, P, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

Myogenin (30-224) antibody

AM08353PU-N at 10 µg/ml staining Myogenin in Skeletal muscle by Immunohistochemistry, Formalin-Fixed Paraffin-Embedded tissue. Mouse IgG 5FD Aff - Purified Hu, Ms, Rt C, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Myogenin (Center) antibody

Western blot analysis in K562 cell line lysates (35ug/lane) using Myogenin antibody Cat.-No. AP52800PU-N. This demonstrates this antibody detected the Myogenin protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Myogenin (N-term) antibody

Western blot analysis in ZR-75-1 cell line lysates (35ug/lane) using Myogenin antibody Cat.-No. AP52801PU-N (N-term). This demonstrates this antibody detected the Myogenin protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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