Background of Myosin light chain 2 (MYL2) antibody
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions.
Immunohistochemistry of paraffin-embedded human heart tissue slide using 10906-1-AP( MYL2 Antibody) at dilution of 1:200 (under 10x lens)
Immunofluorescence: Fast skeletal myosin light chain 1 Antibody (MY-21) [NB120-11082] - Immunofluorescent methods on chicken or human fibroblast cells using NB120-11082. The antibody localizes an epitope on stress fibers of well spread cells.
Immunohistochemistry: AM09080PU-N MYL2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skeletal Muscle followed by biotinylated secondary antibody, alkaline hosphatase-streptavidin and chromogen.
The extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis: The extracts of Rat heart (60 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: Myosin Light Chain 2 Antibody (EPR3741) [NBP1-40754] - Western blot analysis on (A) fetal heart, (B) fetal skeletal muscle, (C) human heart, (D) mouse heart and (E) rat heart lysates using anti-Myosin Light Chain 2
Western Blot: Myosin Light Chain 2 Antibody [NBP1-85541] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Formalin-fixed and paraffin embedded mouse embryonic muscle labeled with Anti-Phospho-MYL(Ser19)Polyclonal Antibody, Unconjugated (bs-3295R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.