MYSM1 antibody

Principal name

MYSM1 antibody

Alternative names for MYSM1 antibody

KIAA1915, Histone H2A deubiquitinase MYSM1, Myb-like SWIRM and MPN domain-containing protein 1

SwissProt ID

A0JMR6 (Xenla), Q5F3F2 (Chick), Q5RGA4 (Danre), Q5VVJ2 (Human), Q69Z66 (Mouse)

Gene ID

114803 (MYSM1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C)

Background of MYSM1 antibody

Metalloprotease that specifically deubiquitites monoubiquitited histone H2A; a specific tag for epigenetic transcriptiol repression; thereby acting as a coactivator. Preferentially deubiquitites monoubiquitited H2A in hyperacetylated nucleosomes. Deubiquitition of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.Component of a large chromatin remodeling complex; at least composed of MYSM1; PCAF; RBM10 and KIF11/TRIP5. Binds histones

General readings

Dephoure, N. et al., Proc. Natl. Acad. Sci. U.S.A. 105(31): 10762-10767 (2008)
Zhu, P. et al., Mol. Cell 27(4): 609-621 (2007)
Matsuoka, S. et al., Science 316(5828): 1160-1166 (2007)
Yoneyama, M. et al., J. Mol. Biol. 369(1): 222-238 (2007)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MYSM1 antibody

Predicted cell location: Nucleus. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using MYSM1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYSM1 antibody

Predicted band size: 95 kDa . Positive control: Mouse kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/1100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MYSM1 antibody

Host: Rabbit; Target Name: MYSM1; Sample Tissue: Fetal Liver lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, Hu, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

MYSM1 antibody

Host: Rabbit; Target Name: MYSM1; Sample Tissue: Hela Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MYSM1 (N-term) antibody

Western blot analysis in MDA-MB231 cell line lysates (35ug/lane) using MYSM1 antibody Cat.-No. AP52802PU-N (N-term). This demonstrates this antibody detected the MYSM1 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MYSM1 antibody

MYSM1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

MYSM1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYSM1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P
50 µg / €205.00
  Assay Biotech

MYSM1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MYSM1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P
0.1 mg / €310.00
  Assay Biotech

MYSM1 antibody

Western Blot: MYSM1 Antibody [NBP1-52625] - Whole cell lysate from Jurkat (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-MYSM1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MYSM1 was more efficiently immunoprecipitated by rabbit anti-MYSM1 antibody NBP1-52626, which recognizes a downstream epitope. Rabbit Aff - Purified Hu WB
0.1 mg / €440.00
  Novus Biologicals Inc.

MYSM1 antibody

Western Blot:  MYSM1 Antibody [NBP1-52626] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells.  Antibodies: Affinity purified rabbit anti-MYSM1 antibody used for IP at 6 mcg/mg lysate. MYSM1 was less efficiently immunoprecipitated by rabbit anti-MYSM1 antibody NBP1-52625, which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MYSM1

MYSM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MYSM1

MYSM1 Human
10 µg / €380.00
  Abnova Taiwan Corp.

MYSM1

MYSM1 Human
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MYSM1 overexpression lysate

MYSM1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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