N4BP1 antibody

Principal name

N4BP1 antibody

Alternative names for N4BP1 antibody

KIAA0615, NEDD4-binding protein 1

SwissProt ID

O75113 (Human), Q1LVK9 (Danre), Q5ZLE9 (Chick), Q6A037 (Mouse), Q7ZXG4 (Xenla)

Gene ID

9683 (N4BP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of N4BP1 antibody

Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin–protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73alpha, a target of Itchmediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73alpha for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73alpha and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

N4BP1 antibody

Western blot analysis of extracts from MCF-7 cells, 293 cells and Jurkat cells, using N4BP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

N4BP1 antibody

Western blot analysis of N4BP1 in HeLa cell lysate with N4BP1 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

N4BP1 antibody

Immunohistochemistry (IHC) analyzes of N4BP1 antibody (Cat.-No.: AP21078PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

N4BP1 antibody

N4BP1 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

N4BP1 antibody

Western blot analysis of extracts from MCF-7/Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

N4BP1 antibody

Western blot analysis of extracts from MCF-7/Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

N4BP1 antibody

Immunohistochemical analysis of N4BP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

N4BP1 antibody

Immunohistochemical analysis of N4BP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

N4BP1 antibody

Immunohistochemical analysis of N4BP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

N4BP1 antibody

Immunohistochemistry-Paraffin: N4BP1 Antibody [NBP1-86233] Immunohistochemical staining of human thyroid gland shows strong nuclear and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

N4BP1 antibody

Immunohistochemistry-Paraffin: N4BP1 Antibody [NBP1-86238] Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts / spermatogonia. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

11 Item(s)

per page
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