NAB2 antibody
Principal name
NAB2 antibody
Alternative names for NAB2 antibody
MADER, NGFI-A-binding protein 2, Melanoma-associated delayed early response protein
SwissProt ID
Q15742 (Human)
Gene ID
4665 (NAB2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)
Background of NAB2 antibody
NAB2 is a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize withThis gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Srinivasan,R., et al., (er) J. Biol. Chem. (2006) In press
Human HepG2; WB Suggested Anti-NAB2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; NAB2 antibody - N-terminal region (ARP38715_T100) in Human HepG2 cells using Western Blot
Detection limit for recombinant GST tagged NAB2 is approximately 1ng/ml as a capture antibody.
Western Blot analysis of NAB2 expression in transfected 293T cell line (H00004665-T01) by NAB2 MaxPab polyclonal antibody.Lane 1: NAB2 transfected lysate(26.51 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NAB2 expression in transfected 293T cell line (H00004665-T01) by NAB2 MaxPab polyclonal antibody.Lane 1: NAB2 transfected lysate(26.51 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry (IHC) analyzes of NAB2 antibody (Cat.-No. AP01179PU-N) in paraffin-embedded human brain tissue.
Western blot analysis of NAB2 antibody (Cat.-No. AP01179PU-N) in extracts from Raw264.7 cells at 1/500 dilution
Human Heart; WB Suggested Anti-NAB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; NAB2 antibody - C-terminal region (ARP38716_P050) in Human Heart cells using Western Blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from HT-29/RAW264.7 cells, using NAB2 Antibody Cat.-No AP32263PU-N. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of NAB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of NAB2 expression in HepG2 (A); HeLa (B); Raw264.7 (C) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803848)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NAB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803848)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-NAB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803848)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NAB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803957)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803957)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NAB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804068)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804068)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAB2 (RC209554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAB2.
Western blot analysis of extracts from HT-29 cells and RAW264.7 cells, using NAB2 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 3T3 cells (Lane 2), colo cells (Lane 3) and K562 cells (Lane 4), using NAB2 Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using NAB2 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis on HT29 cell lysate using NAB2 Antibody
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA803848 | NAB2 antibody |
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| NAB2 mouse monoclonal antibody, clone OTI5D7 (formerly 5D7) | |||||||
 |
Mouse | IgG1 | OTI5D7 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA314231 | NAB2 antibody |
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| Rabbit polyclonal anti-NAB2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA347682 | NAB2 antibody |
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| Rabbit Polyclonal Anti-NAB2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA803954 | NAB2 antibody |
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| NAB2 mouse monoclonal antibody, clone OTI6D10 (formerly 6D10) | |||||||
 |
Mouse | IgG1 | OTI6D10 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA803955 | NAB2 antibody |
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| NAB2 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1) | |||||||
 |
Mouse | IgG1 | OTI1E1 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803956 | NAB2 antibody |
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| NAB2 mouse monoclonal antibody, clone OTI5D10 (formerly 5D10) | |||||||
 |
Mouse | IgG2a | OTI5D10 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA803957 | NAB2 antibody |
||||||
| NAB2 mouse monoclonal antibody, clone OTI3H5 (formerly 3H5) | |||||||
 |
Mouse | IgG1 | OTI3H5 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA804060 | NAB2 antibody |
||||||
| NAB2 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6) | |||||||
 |
Mouse | IgG1 | OTI1C6 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA804061 | NAB2 antibody |
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| NAB2 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10) | |||||||
 |
Mouse | IgG2a | OTI1B10 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA804068 | NAB2 antibody |
||||||
| NAB2 mouse monoclonal antibody, clone OTI1E12 (formerly 1E12) | |||||||
 |
Mouse | IgG2b | OTI1E12 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA804069 | NAB2 antibody |
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| NAB2 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3) | |||||||
 |
Mouse | IgG1 | OTI1H3 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| AP01179PU-N | NAB2 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€350.00
|
||
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP32263PU-N | NAB2 antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, P, WB |
50 µl /
€380.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| C11102-1 | NAB2 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | E, P, WB |
50 µg /
€230.00
|
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| Assay Biotech | |||||||
| +1 additional image |
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| C11102-2 | NAB2 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms | E, P, WB |
0.1 mg /
€330.00
|
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| Assay Biotech | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN209554 | NAB2 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209554BN | NAB2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209554LP | NAB2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209554RB | NAB2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309554 | NAB2 |
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| Recombinant protein of human NGFI-A binding protein 2 (EGR1 binding protein 2) (NAB2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
