NADP-ME antibody

Principal name

NADP-ME antibody

Alternative names for NADP-ME antibody

ME1, NADP-dependent malic enzyme

SwissProt ID

P06801 (Mouse), P13697 (Rat), P48163 (Human), Q29558 (Pig), Q92060 (Chick)

Gene ID

4199 (ME1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Can (Canine), Eq (Equine), Rb (Rabbit), Sh (Sheep), GP (Guinea Pig), Gt (Goat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NADP-ME antibody

ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Hsieh,J.Y., (2006) J. Biol. Chem. 281 (32), 23237-23245

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NADP-ME antibody

Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. C: BCL-1. 7.5% SDS PAGE. TA308668 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NADP-ME antibody

Immunoperoxidase of monoclonal antibody to ME1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.5 ug/ml] Mouse IgG1 3H5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NADP-ME antibody

Western blot analysis of extracts from HeLa cells, using ME1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NADP-ME antibody

WB Suggested Anti-ME1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NADP-ME antibody

Host: Rabbit; Target Name: ME1; Sample Tissue: Lymph Node Tumor lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

NADP-ME antibody

Western blot analysis of ME1 in 293 cell lysate with ME1 antibody at  1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NADP-ME antibody

Western blot (WB) analysis of Malic enzyme 1 antibody in extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NADP-ME antibody

Western Blot analysis of ME1 expression in transfected 293T cell line (H00004199-T01) by ME1 MaxPab polyclonal antibody.

Lane 1: ME1 transfected lysate(64.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NADP-ME antibody

ME1 monoclonal antibody (M01), clone 2A2. Western Blot analysis of ME1 expression in human stomach. Mouse IgG2b 2A2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NADP-ME antibody

Detection limit for recombinant GST tagged ME1 is approximately 3ng/ml as a capture antibody. Mouse IgG2b 3B4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NADP-ME antibody

Western blot analysis of extracts from HeLa cells, using ME1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

NADP-ME antibody

Western blot analysis of extracts from HeLa cells, using ME1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

NADP-ME antibody

Human Bronchial Epithelial Tissue; Rabbit Anti-ME1 Antibody . Catalog Number: ARP32794_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue . Observed Staining: Cytoplasmic . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-ME1 Antibody (ARP32794_P050) ) in Human Bronchial Epithelial Tissue using Immunohistochemistry Rabbit Aff - Purified Bov, Chk, Hu, Por WB
50 µg / €350.00
  AVIVA Systems Biology

NADP-ME antibody

NADP-ME Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

NADP-ME antibody

NADP-ME Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

NADP-ME antibody

Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-86129] - Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NADP-ME

NADP-ME Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NADP-ME

NADP-ME Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NADP-ME

NADP-ME Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ME1 overexpression lysate

ME1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

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