NAGK antibody

Principal name

NAGK antibody

Alternative names for NAGK antibody

N-acetyl-D-glucosamine kinase

SwissProt ID

P81799 (Rat), Q9QZ08 (Mouse), Q9UJ70 (Human)

Gene ID

55577 (NAGK), 4055577 (atpH)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of NAGK antibody

N-acetylglucosamine kinase (NAGK) converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent salvage enzyme of amino sugar metabolism in mammals. The predicted 344-amino acid NAGK protein contains the 5 sequence motifs necessary for the binding of ATP by sugar kinases. NAGK shares 91.6% amino acid similarity with mouse Nagk, for which enzyme activity is detectable in all mouse tissues examined, with highest enzymatic activity in testis. It is hypothesized that NAGK has a general role in the catabolic pathways of GlcNAc as well as of ManNAc.

General readings

Weihofen WA, Berger M, Chen H, et al. (2007). J. Mol. Biol. 364 (3): 388–99.Weidanz JA, Campbell P, Moore D, et al. (1997). Br. J. Haematol. 95 (4): 645–53.Hinderlich S., et al. (Jul 2000). Eur J Biochem 267 (11): 3301–8.

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NAGK antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT4D12 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

NAGK antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT4D12 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

NAGK (N-term) antibody

The anti-NAGK Pab is used in Western blot to detect NAGK in mouse thymus tissue lysate (Lane 1) and Y79 cell lysate (Lane 2). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NAGK (C-term) antibody

Western blot analysis of NAGK (arrow) in 293 cell line lysates (35ug/lane) using NAGK antibody Cat.-No. AP13659PU-N (C-term). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NAGK antibody

NAGK polyclonal antibody (A01), Lot # 060102JC01. Western Blot analysis of NAGK expression in human ovary cancer. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NAGK antibody

NAGK Mouse IgG2b 1H8 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NAGK antibody

The NAGK polyclonal antibody ( Cat # PAB4634 ) is used in Western blot to detect NAGK in mouse thymus tissue lysate . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NAGK antibody

NAGK Rabbit Hu, Ms E
0.4 ml / €390.00
  Abnova Taiwan Corp.

NAGK antibody

Western Blot: N acetylglucosamine kinase Antibody [NBP1-42694]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-NAGK antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  NAGK was also immunoprecipitated by another rabbit anti-NAGK antibody, which recognizes an upstream epitope. Rabbit Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

NAGK antibody

Immunohistochemistry-Paraffin: N acetylglucosamine kinase Antibody [NBP1-89750] - Immunohistochemical staining of human cerebellum shows distinct nuclear membranous positivity in Purkinje cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NAGK

NAGK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NAGK (1-344, His-tag)

NAGK Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

NAGK (1-344, His-tag)

NAGK Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

NAGK

NAGK Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

NAGK

NAGK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NAGK

NAGK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

16 Item(s)

per page
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