NAK1 antibody

Principal name

NAK1 antibody

Alternative names for NAK1 antibody

NR4A1, GFRP1, HMR, NUR77, ST-59, N10, Orphan Nuclear Receptor HMR, Early Response Protein NAK1, TR3 Orphan Receptor

SwissProt ID

P12813 (Mouse), P22736 (Human), P22829 (Rat), Q0V8F0 (Bovin)

Gene ID

3164 (NR4A1), 15370 (Nr4a1)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of NAK1 antibody

Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis. Nak1 is a phosphoprotein and its size ranges from 67 kD to 88 kD, depending on post-translational modifications.

Nak1, a NR4 Nerve Growth Factor-Like receptor, has been shown to affect the hypothalamic-pituitary axis, neuronal differentiation, neuroendocrine regulation of adrenocortical function, and T-cell apoptosis. Nak1 also is known as nuclear receptor Tr3 and is similar to mouse Nur77 and rat NGFI-B. It is encoded by one of the immediate early genes (IEG) in response to nerve growth factor and other serum growth factors. Nak1 can bind to DNA as a monomer, homodimer, or heterodimer (with retinoid X receptors). Nak1 has been shown to inhibit vascular lesion formation and could serve as a novel target in the treatment of vascular diseases.

General readings

Kiss I, Oskolas H, Toth R, Bouillet P, Toth K, Fulop A, Scholtz B, Ledent C, Fesus L, Szondy Z. Adenosine A(2A )receptor-mediated cell death of mouse thymocytes involves adenylate cyclase and Bim and is negatively regulated by Nur77. Eur J Immunol. 2006 Jun;36(6):1559-71. PMID: 16673448

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NAK1 antibody

WB Suggested Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

Human Testis; NR4A1 antibody - N-terminal region (ARP31941_T100) in Human Testis cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

NAK1 (Isoform A) antibody

TA303143 (2µg/ml) staining of Human Thyroid lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

Western blot analysis of extracts from COS-7 cells, using Nuclear Receptor NR4A1 (Ab-351) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

Immunoblotting of PMA and ionomycin-stimulated (lane 1) and unstimulated mouse thymocytes (lane 2) with 5 ug/mL of Anti-Mouse Nur77 Purified.  The band was visualized using Rat Anti-Mouse IgG HRP. Mouse 12.14 Purified Ms WB
25 µg / €120.00
  OriGene Technologies, Inc.

NAK1 antibody

WB Suggested Anti-NR4A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

WB Suggested Anti-NR4A1 Antibody; Titration: 1.0 ug/ml; Positive Control: HepG2 Whole Cell Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

Western Blot: NUR77 Antibody [TA336473] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution. Rabbit Purified Hu, Ms ChIP, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

Host: Rabbit; Target Name: NR4A1; Sample Tissue: Liver Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

WB Suggested Anti-NR4A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

NAK1 Rabbit Purified Hu, Mky
50 µg / €379.00
  OriGene Technologies, Inc.

NAK1 antibody

Anti-NUR77 antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Bov, Can, Eq, Hu, Mky
50 µg / €379.00
  OriGene Technologies, Inc.

NAK1 (Internal Region) antibody

Anti-NUR77 antibody IHC of human primary follicles. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Can, Eq, Hu, Mky, Ms, Prim, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

NAK1 antibody

Immunohistochemistry analysis of Nur77/NR4A1 Antibody Cat.-No AP01460PU-N in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NAK1 pSer351 antibody

Dot blot analysis of Phospho-NR4A1-S351 polyclonal antibody (Cat#AP12790PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

NAK1 antibody

Western blot analysis of NR4A1 (arrow) using rabbit polyclonal NR4A1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NR4A1 gene (Lane 2) Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NAK1 pSer351 antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody AP55732PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 ml / €340.00
  Acris Antibodies GmbH

NAK1 pSer351 antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody AP55732PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
50 µl / €270.00
  Acris Antibodies GmbH

NAK1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between NR4A1 and RPS6KA5 HeLa cells were stained with anti-NR4A1 rabbit purified polyclonal 1:1200 and anti-RPS6KA5 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NAK1 antibody

Western blot analysis of NR4A1 in 15 µgs of NIH/3T3 cell lysate. Using NR4A1 polyclonal antibody ( Cat # PAB0352 ) at 1 : 500 dilution. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

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