NAK1 antibody

Principal name

NAK1 antibody

Alternative names for NAK1 antibody

NR4A1, GFRP1, HMR, NUR77, ST-59, N10, Orphan Nuclear Receptor HMR, Early Response Protein NAK1, TR3 Orphan Receptor

SwissProt ID

P12813 (Mouse), P22736 (Human), P22829 (Rat), Q0V8F0 (Bovin)

Gene ID

3164 (NR4A1), 15370 (Nr4a1)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish), Mky (Monkey), Bt (Bat), Prim (Primate), Bear

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), ChIP assay (ChIP), Immunoprecipitation (IP)

Background of NAK1 antibody

NR4A1(NUCLEAR RECEPTOR SUBFAMILY 4, GROUP A, MEMBER 1), also called NAK1, GFRP1, TR3, NUR77 or NGFIB, is a protein that in humans is encoded by the NR4A1 gene, which is also a member of the Nur nuclear receptor family of intracellular transcription factors. The NR4A1 gene is mapped on 12q13.13. NR4A1 is involved in cell cycle mediation, inflammation and apoptosis. It plays a key role in mediating inflammatory responses in macrophages. In addition, subcellular localization of the NR4A1 protein appears to play a key role in the survival and death of cells. Nr4a1 was overexpressed in Wnt1 -transformed mouse mammary cells. Nr4a1 was also induced by lithium, a Wnt1 mimic, and the Nr4a1 promoter was activated by lithium and beta-catenin, a Wnt1 downstream effector. In contrast, human NR4A1 was not upregulated by beta-catenin, indicating that this gene is regulated differently in human and mouse cells. Adenoviral expression of Nr4a1 induced genes involved in gluconeogenesis, stimulated glucose production both in vitro and in vivo, and raised blood glucose levels.

General readings

Lu,L., et al., (2004) J. Clin. Endocrinol. Metab. 89 (8), 4113-4118

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NAK1 antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody  
WB Suggested Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NAK1 (Isoform A) antibody

  Goat Polyclonal Antibody against NR4A1  
TA303143 (2µg/ml) staining of Human Thyroid lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit polyclonal Nuclear Receptor NR4A1 (Ser351)(Phospho-specific)  
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit polyclonal Nuclear Receptor NR4A1 (Ab-351) antibody  
Western blot analysis of extracts from COS-7 cells, using Nuclear Receptor NR4A1 (Ab-351) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Mouse Anti-Mouse Nur77 Purified (25 ug)  
Immunoblotting of PMA and ionomycin-stimulated (lane 1) and unstimulated mouse thymocytes (lane 2) with 5 ug/mL of Anti-Mouse Nur77 Purified.  The band was visualized using Rat Anti-Mouse IgG HRP. Mouse 12.14 Purified Ms WB
25 µg / €120.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody  
WB Suggested Anti-NR4A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody  
WB Suggested Anti-NR4A1 Antibody; Titration: 1.0 ug/ml; Positive Control: HepG2 Whole Cell Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit Polyclonal NGFI-B alpha/Nur77/NR4A1 Antibody  
Western Blot: NUR77 Antibody [TA336473] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution. Rabbit Purified Hu, Ms ChIP, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody  
Host: Rabbit; Target Name: NR4A1; Sample Tissue: Liver Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody  
WB Suggested Anti-NR4A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody (N-Terminus)  
NAK1 Rabbit Purified Hu, Mky
50 µg / €400.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody (N-Terminus)  
Anti-NUR77 antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Bov, Can, Eq, Hu, Mky
50 µg / €400.00
  OriGene Technologies, Inc.

NAK1 (Internal Region) antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody (Internal)  
Anti-NUR77 antibody IHC of human primary follicles. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Can, Eq, Hu, Mky, Ms, Prim, Rt P
50 µg / €400.00
  OriGene Technologies, Inc.

NAK1 antibody

  Rabbit Polyclonal Anti-NR4A1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: PC3 cells, Primary antibody: (NR4A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NAK1 antibody

  Mouse Monoclonal anti-Nur77 Antibody  
Total cell lysates from untreated Jurkat cells (Lane 1) or Jurkat cells treated with PMA and calcium ionophore (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Nur77 (clone 1E10A15) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse 1E10A15 Purified Hu ICC/IF, WB
0.1 mg / €248.00
  OriGene Technologies, Inc.

NAK1 antibody

Immunohistochemistry analysis of Nur77/NR4A1 Antibody Cat.-No AP01460PU-N in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NAK1 pSer351 antibody

Dot blot analysis of Phospho-NR4A1-S351 polyclonal antibody (Cat#AP12790PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

NAK1 antibody

Western blot analysis of NR4A1 (arrow) using rabbit polyclonal NR4A1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NR4A1 gene (Lane 2) Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NAK1 pSer351 antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody AP55732PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 ml / €340.00
  Acris Antibodies GmbH

NAK1 pSer351 antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody AP55732PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
50 µl / €270.00
  Acris Antibodies GmbH

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