NALP3 antibody

Principal name

NALP3 antibody

Alternative names for NALP3 antibody

NLRP3, C1orf7, CIAS1, PYPAF1, NACHT, LRR and PYD domains-containing protein 3

SwissProt ID

Q8R4B8 (Mouse), Q96P20 (Human)

Gene ID

114548 (NLRP3), 216799 (Nlrp3)

Available reactivities

Hu (Human), Bov (Bovine), Rt (Rat), Can (Canine), Ms (Mouse), Por (Porcine), Prim (Primate)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P)

Background of NALP3 antibody

NLRP3/LP3 belongs to the NLR family of inte immune cytoplasmic receptors (reviewed in De rdo and Latz, 2011; Kanneganti, 2010; And, 2011). NLR family members contain an N-terminus effector region consisting of either a pyrin (PYD), a CARD, or a BIR domain. NLRs also have an intermediate conserved NOD domain and a C-terminus LRR domain. NLRs, like TLRs, alert the immune system to the presence of PAMPs and DAMPs. Like a number of other NLRs, NLRP3 functions in a multiprotein complex called the inflammasome. The inclusion of a particular NLR within an inflammasome depends upon the upstream trigger. The NLRP3 inflammasome is considered to be a global sensor and assembles in response to a broad spectrum of PAMPs and DAMPs. For example, helmith, bacteria, plasmodium and viruses have all been shown to activate NLRP3 (reviewed in Menu and Vince, 2011). The PYD of activated NLRP3 associates with the PYD of ASC, triggering the CARD of ASC to associate with the CARD of caspase-1 and completes the assembly of the NLRP3 inflammasome. This serves as a platform for caspase-1 activation. Activation of NLRP3 can be inhibited by glybenclamide (also known as glyburide) (reviewed in Lamkanfi et al, 2009). Glybenclamide is an ATP-sensitive potassion channel inhibitor that has been shown to inhibit various biological processes including activation of the NRLP3 inflammasome. Glybenclamide is thought to act downstream of the P2X7 receptor but upsteam of NLRP3. Glybenclamide blocks activation of caspase-1 and IL-1beta K+ efflux inhibition. Glybenclamide has a number of additiol functions besides blocking including enhancing insulin action and stimulating synthesis of glucose transporters. Researchers are encouraged to consult the published literature regarding additiol information about how glybenclamide perturbs biological processes.

General readings

1. Hoffman HM, Mueller JL, Broide DH, Wanderer AA, Kolodner RD. Mutation of a new gene encoding a putative pyrin-like protein causes familial cold autoinflammatory syndrome and Muckle-Wells syndrome. Nat Genet. 2001 Nov;29(3):301-5.
PMID: 11687797

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NALP3 antibody

AP32694PU-N (2 µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NALP3 antibody

Sample(30 ug of whole cell lysate). A:Raji. 7.5% SDS PAGE. TA309046 diluted at 1:1500 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NALP3 antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus and thyroid cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using NLRP3 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NALP3 antibody

Western Blot: NALP3 Antibody (25N10E9) [TA336883] - Analysis using the NALP3 monoclonal antibody. (A), (B), mouse RAW lysate © and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. goat anti-mouse Ig HRP s Mouse IgG2b 25N10E9 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NALP3 antibody

Western Blot: NALP3 Antibody [TA336895] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/ml respectively. goat anti-r Rabbit Purified Hu, Ms, Prim, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NALP3 antibody

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 cells, hela cells, Primary antibody: (NLRP3 Antibody) at dilution 1/88, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NALP3 antibody

The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (NLRP3 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NALP3 (N-term) antibody

Western blot analysis of NLRP3 Antibody (N-term) (AP18137PU-N) in MDA-MB231 cell line lysates (35ug/lane).NLRP3 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NALP3 (C-term) antibody

AP22372PU-N NLRP3 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Peripheral Blood Leukocytes at 2.5 µg/ml using Biotinylated anti-Goat IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Goat Aff - Purified Bov, Hu, Rt E, F
0.1 mg / €350.00
  Acris Antibodies GmbH

NALP3 antibody

NALP3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NALP3 antibody

Western Blot analysis of NLRP3 expression in transfected 293T cell line (H00114548-T01) by NLRP3 MaxPab polyclonal antibody.

Lane 1: CIAS1 transfected lysate(113.96 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

NALP3 antibody

Western Blot analysis of NLRP3 expression in transfected 293T cell line (H00114548-T02) by NLRP3 MaxPab polyclonal antibody.

Lane 1: NLRP3 transfected lysate(118.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NALP3 antibody

Western Blot analysis of CIAS1 expression in transfected 293T cell line by CIAS1 monoclonal antibody (M01), clone 3B1.

Lane 1: CIAS1 transfected lysate(118.2 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 3B1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NALP3 antibody

Immunofluorescence microscopy showing the detection of endogenous NLRP3 in THP-1 cells with NLRP3 monoclonal antibody, clone Nalpy3-a ( Cat # MAB3723 ). Mouse IgG1 Nalpy3-a Hu ICC/IF, WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

NALP3 antibody

NALP3 Mouse IgG1 Nalpy3-b Hu C, IP, WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

NALP3 antibody

NALP3 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

NALP3 antibody

NALP3 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

NALP3 antibody

NALP3 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NALP3

NALP3 Human
2 µg / €300.00
  Abnova Taiwan Corp.

NALP3

NALP3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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