NANOG antibody

Principal name

NANOG antibody

Alternative names for NANOG antibody

Homeobox protein NANOG

SwissProt ID

Q4JM65 (Bovin), Q5TM83 (Musmm), Q5TM84 (Macfa), Q80Z64 (Mouse), Q9H9S0 (Human)

Gene ID

79923 (NANOG), 100293888 (LOC100293888), 71950 (Nanog)

Available reactivities

Hu (Human), Por (Porcine), Ms (Mouse), Chimp (Chimpanzee), Gt (Goat), Mky (Monkey), Bov (Bovine), Can (Canine), Rt (Rat), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Dot blot (Dot)

Background of NANOG antibody

Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).

General readings

1. Raya A et.al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature. 2009 Jul 2;460(7251):53-9. PMID: 19483674
2. Montserrat N, Garreta E, González F, Gutiérrez J, Eguizábal C, Ramos V, Borrós S, Izpisua Belmonte JC. Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system.
J Biol Chem. 2011 Apr 8;286(14):12417-28. PMID: 21285354
3. Montserrat N, Bahima EG, Batlle L, Häfner S, Rodrigues AM, González F, Belmonte JC.
Generation of pig iPS cells: a model for cell therapy. J Cardiovasc Transl Res. 2011 Apr;4(2):121-30. PMID: 21088946
4. Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, Raya A, Belmonte JC. Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions.
Stem Cells. 2010 Jan;28(1):36-44. PMID: 19890879
5. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC. Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Nat Biotechnol. 2008 Nov;26(11):1276-84. PMID: 18931654
6. Montserrat N, Ramírez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, González F, Izpisua Belmonte JC, Campistol JM. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2. J Biol Chem. 2012 Jul 13;287(29):24131-8. PMID: 22613719

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NANOG (144-157) antibody

TA303048 (0.03µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG (N-term) antibody

Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#TA302153) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#TA302153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-NANOG rabbit polyclonal antibody. (TA590448) Rabbit IgG Purified Hu Dot, E, ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NANOG antibody

Western Blot (Reduced) using NANOG Antibody Cat.-No AP01140BT Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

NANOG antibody

Western Blot (Reduced) using NANOG Antibody Cat.-No AP01140BT Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

NANOG antibody

Formalin-Fixed, Paraffin-Embedded Sections of human prostate malignant adenocarcinoma stained with NANOG Antibody Cat.-No AP01140PU Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NANOG antibody

Formalin-Fixed, Paraffin-Embedded Sections of human prostate malignant adenocarcinoma stained with NANOG Antibody Cat.-No AP01140PU Rabbit Aff - Purified Hu E, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

NANOG antibody

Human Spleen (formalin-fixed, paraffin-embedded) stained with NANOG antibody AP22771PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NANOG (101-131) antibody

NANOG Antibody (Center) (Cat. #TA302154) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow). Rabbit Ig Purified Hu ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG (C-term) antibody

TA303206 (0.05µg/ml) staining of HeLa (nuclear) cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG (Center) antibody

Western blot analysis of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG antibody

NTERA cell line lysate was loaded at 1x10e5 cells/lane, probed with 1 ug/mL of Anti-Human Nanog Purified and revealed with Anti-Mouse IgG HRP. Mouse hnog.2 Purified Hu WB
25 µg / €150.00
  OriGene Technologies, Inc.

NANOG antibody

Predicted band size: 35 kDa . Positive control: Human Umbilical cord mesenchymal stem cell lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE All lane: Human Umbilical cord mesenchymal stem cell Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NANOG antibody

Western blot analysis of NANOG (arrow) using rabbit NANOG Antibody (S285) (Cat.# TA324577). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies). Rabbit IgG Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NANOG antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human prostate malignant adenocarcinoma.  The recommended concentration is 0.5 mg/ml with an overnight incubation at 4C.  An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG (mouse 270-284) antibody

TA334190 (0.5µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG antibody

Western blot detection of Nanog in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Nanog polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Peptide absorption control lane 3. Rabbit Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG antibody

Western Blot: Nanog Antibody [TA336515] - Western blot detection of Nanog in 20 ug of human hippocampus tissue lysate (lanes 2 and 4) with Nanog polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker lane 1. Peptid Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NANOG antibody

Western Blot: Nanog Antibody (1E6C4) [TA336771] - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2). Mouse IgG1 1E6C4 Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NANOG antibody

Immunohistochemistry-Paraffin: Nanog Antibody [TA336902] - IHC staining of Nanog in mouse testis using DAB with hematoxylin counterstain. Rabbit Purified Ms C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

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