Napsin-A antibody

Principal name

Napsin-A antibody

Alternative names for Napsin-A antibody

NAPSA, NAP1, Napsin-1,Aspartyl protease 4, TA01/TA02

SwissProt ID

O09043 (Mouse), O96009 (Human)

Gene ID

9476 (NAPSA), 4353 (MPO)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of Napsin-A antibody

Napsin A gene codes for two aspartic proteinases precursor generally expressed in lungs and kidneys and its translational product is fully functional, glycosylated aspartic proteinase containing a RGF motif and an additional 18 residues at its C-terminal end. The Napsin B gene is expressed in exclusively in cells of the immune system and is expressed in several polymorphic form (1). Members of the aspartic protease family have been implicated in cancer progression. The aspartic protease napsin A is expressed in type II cells of the lung, where it is involved in the processing of surfactant protein B (SP-B). Napsin A expression in kidney is decrease in adenocarcinomas this result is consistent with earlier observations of loss of napsin A in high grade lung carcinomas (2). Mutation in the aspartic acid residue resulted in complete loss of enzymatic activity but it did not influence glycosylation, processing and intracellur localization.
Re-expression of napsin A in HEK-293 cells inhibit the tumor growth by mechanism independent of aspartic proteinase activity (2).
Interstitial lung disease (ILD) ail lung in children represent a rare heterogeneous group of respiratory disorders characterized by restrictive lung disease and diffuse infiltrates. Mutations in the surfactant proteins (SP-A, SP-B, SP-C and SP-D) and in the ABC binding cassette protein A3 (ABCA3) are found to be associated with pediatric ILD (3). While SP-A and SP-D play essential roles in the pulmonary immune defense, SP-B and SP-C lower the surface-tension and facilitate formation and stabilization of the surfactant film in young lungs. SP-B is expressed in type II non-ciliated bronchiolar epithelial cells in pre-pro-protein of 381 amino acids. After removal of 23amio acids form N-terminal end the pro-protein is proteolytically processed at the amino and carboxy terminal ends to mature SP-B protein of 79 amino acids by a cystein protease, cathapsin H and aspartylprotease Napsin (4). Napsin A is highly expressed in type II non-ciliated epithelial cells and is implicated in processing of the SP-B and SP-C pro-peptides (4).

General readings

Aulakh KS., et al. (2013) Arch. Pathol. Lab. Med. 137 (8), 1094-1098Chernock RD, et al. (2013) Am. J. Surg. Pathol. 37 (8), 1215-1222

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Napsin-A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAPSA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808666)(1:150) Mouse IgG2b OTI3E5 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Napsin-A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAPSA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808718)(1:150) Mouse IgG2b OTI9F1 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Napsin-A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAPSA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808724)(1:150) Mouse IgG2a OTI3C6 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Napsin-A antibody

Formalin-Fixed, Paraffin-Embedded Human kidney stained with Napsin-A Antibody Cat.-No AP32830PU. Rabbit IgG Purified Hu F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Napsin-A antibody

Formalin-Fixed, Paraffin-Embedded Human kidney stained with Napsin-A Antibody Cat.-No AP32830PU. Rabbit IgG Purified Hu F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Napsin-A antibody

Formalin-Fixed, Paraffin-Embedded Human kidney stained with Napsin-A Antibody Cat.-No AP32830PU. Rabbit IgG Purified Hu F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

Napsin-A antibody

Western Blot - Western blot analysis of NAPSIN1 expression in human lung using anti-NAPSIN1. Mouse IgG1 TMU-Ad02 Purified Hu C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Napsin-A antibody

Immunohistochemistry staining of Paraffin Lung adenocarcinoma, kidney tissue by Napsin A antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Rabbit Purified Hu P
1 ml / €590.00
  OriGene Technologies, Inc.

Napsin-A antibody

Immunohistochemistry staining of Paraffin Lung adenocarcinoma, renal cell carcinoma tissue by Napsin A antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Mouse IgG1 MRQ-60 Purified Hu P
1 ml / €210.00
  OriGene Technologies, Inc.

Napsin-A antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NAPSA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10 min, TA807998)(1:150) Mouse IgG2b OTI2F11 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Napsin-A antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAPSA (RC204715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAPSA.(1:2000) Mouse IgG1 OTI1B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Napsin-A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAPSA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808773)(1:150) Mouse IgG2a OTI3A4 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Napsin-A antibody

Napsin-A Mouse IgG1 KCG1.1 Supernatant Hu C, P
6 ml / €420.00
  Acris Antibodies GmbH

Napsin-A (N-term) antibody

Formalin fixed paraffin embedded human lung adenocarcinoma stained with Napsin A monoclonal antibody (AM03172PU) Mouse IgG1 KCG1.1 Purified Hu P
1 ml / €740.00
  Acris Antibodies GmbH

Napsin-A (N-term) antibody

Formalin fixed paraffin embedded human lung adenocarcinoma stained with Napsin A monoclonal antibody (AM03172PU) Mouse IgG1 KCG1.1 Purified Hu P
0.5 ml / €450.00
  Acris Antibodies GmbH

Napsin-A control peptide

Napsin-A
0.25 mg / €250.00
  Acris Antibodies GmbH

Napsin-A (N-term) antibody

Napsin-A Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Napsin-A antibody

Napsin-A Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

Napsin-A antibody

Western Blot analysis of NAPSA expression in transfected 293T cell line (H00009476-T02) by NAPSA MaxPab polyclonal antibody.

Lane 1: NAPSA transfected lysate(46.2 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Napsin-A antibody

Napsin-A Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

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