NARF antibody

Principal name

NARF antibody

Alternative names for NARF antibody

Nuclear prelamin A recognition factor

SwissProt ID

Q2YDU6 (Rat), Q9CYQ7 (Mouse), Q9UHQ1 (Human), Q9UHQ1-2

Gene ID

26502 (NARF), 67608 (Narf), 360681

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of NARF antibody

Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. NARF is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases.Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

General readings

Lattanzi,G., (2007) J. Cell. Biochem. 102 (5), 1149-1159

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NARF antibody

  Rabbit Polyclonal Anti-NARF Antibody  
WB Suggested Anti-NARF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Spleen Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NARF antibody

  Rabbit Polyclonal Anti-NARF Antibody  
WB Suggested Anti-NARF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

NARF - human gene knockout kit via CRISPR

NARF - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Narf - mouse gene knockout kit via CRISPR

Narf - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Narf - mouse gene knockout kit via CRISPR

Narf - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Narf - mouse gene knockout kit via CRISPR

Narf - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Narf - mouse gene knockout kit via CRISPR

Narf - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NARF (transcript variant 1)

  Recombinant protein of human nuclear prelamin A recognition factor (NARF), transcript variant 1  
NARF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NARF (transcript variant 3)

  Recombinant protein of human nuclear prelamin A recognition factor (NARF), transcript variant 3  
NARF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

NARF overexpression lysate

NARF overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

NARF overexpression lysate

NARF overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NARF (mGFP-tagged) - Human nuclear prelamin A recognition factor (NARF), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, NARF (mGFP-tagged) - Human nuclear prelamin A recognition factor (NARF), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,060.00
  OriGene Technologies, Inc.

Lenti ORF particles, NARF (mGFP-tagged)-Human nuclear prelamin A recognition factor (NARF), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, NARF (mGFP-tagged)-Human nuclear prelamin A recognition factor (NARF), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, NARF (mGFP-tagged)-Human nuclear prelamin A recognition factor (NARF), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, NARF (mGFP-tagged)-Human nuclear prelamin A recognition factor (NARF), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, NARF (Myc-DDK tagged) - Human nuclear prelamin A recognition factor (NARF), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, NARF (Myc-DDK tagged) - Human nuclear prelamin A recognition factor (NARF), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €950.00
  OriGene Technologies, Inc.

Lenti ORF particles, NARF (Myc-DDK-tagged)-Human nuclear prelamin A recognition factor (NARF), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, NARF (Myc-DDK-tagged)-Human nuclear prelamin A recognition factor (NARF), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, NARF (Myc-DDK-tagged)-Human nuclear prelamin A recognition factor (NARF), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, NARF (Myc-DDK-tagged)-Human nuclear prelamin A recognition factor (NARF), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Narf (GFP-tagged) - Mouse nuclear prelamin A recognition factor (Narf), 200 uL, >10^7 TU/mL

Lenti ORF particles, Narf (GFP-tagged) - Mouse nuclear prelamin A recognition factor (Narf), 200 uL, >10^7 TU/mL  
200 µl / €1,070.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Narf (GFP-tagged ORF) - Rat nuclear prelamin A recognition factor (Narf), 200 uL, >10^7 TU/mL

Lenti ORF particles, Narf (GFP-tagged ORF) - Rat nuclear prelamin A recognition factor (Narf), 200 uL, >10^7 TU/mL  
200 µl / €1,060.00
  OriGene Technologies, Inc.

Lenti ORF particles, Narf (Myc-DDK-tagged ORF) - Rat nuclear prelamin A recognition factor (Narf), 200 uL, >10^7 TU/mL

Lenti ORF particles, Narf (Myc-DDK-tagged ORF) - Rat nuclear prelamin A recognition factor (Narf), 200 uL, >10^7 TU/mL  
200 µl / €948.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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