NBL1 antibody

Principal name

NBL1 antibody

Alternative names for NBL1 antibody

DAN, DAND1, Neuroblastoma suppressor of tumorigenicity 1

SwissProt ID

P41271 (Human), Q06880 (Rat), Q61477 (Mouse)

Gene ID

17965 (Nbl1), 100532736 (C1orf151-NBL1), 4681 (NBL1)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Rb (Rabbit)

Available hosts

Rat, Mouse, Goat, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of NBL1 antibody

DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in srctransformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteinerich domain that is structurally related to the cysteineknot motif found in TGFβ superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberusrelated), Dante and PRDC (protein-related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN have been shown to bind BMP4 in a functional ELISA. It is likely the various DAN family members and other TGFβ BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGFβ superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGFβ superfamily ligands.

General readings

Olakowski, M., et al. (2009) Folia Histochem Cytobiol 47(2): 249-55.
Shaikhibrahim, Z., et al. (2011) Int J Mol Med 28(4): 605-11.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NBL1 (21-32) antibody

AP32973PU-N (0.1µg/ml) staining of Kelly lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NBL1 antibody

Western blot analysis of extracts from Jurkat cells and COLO cells, using NBL1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NBL1 antibody

WB Suggested Anti-NBL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NBL1 antibody

The cell lysate of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT38G8 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

NBL1 antibody

The cell lysate of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT38G8 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

NBL1 antibody

NBL1 Rat IgG2 7K12 Purified Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

NBL1 antibody

Immunofluorescent analysis of NBL1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NBL1 antibody

Immunofluorescent analysis of NBL1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NBL1 antibody

Immunofluorescent analysis of NBL1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NBL1 (transcript variant 2)

NBL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

NBL1 overexpression lysate

NBL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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