NCAPH antibody

Principal name

NCAPH antibody

Alternative names for NCAPH antibody

BRRN, BRRN1, CAPH, KIAA0074, Chromosome-associated protein H

SwissProt ID

Q15003 (Human)

Gene ID

23397 (NCAPH), 3123397 (Q6LTL4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), African clawed frog, Chimp (Chimpanzee), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of NCAPH antibody

BRNN1, also called Barren, Condensin subunit 2, Barren homolog protein 1, Chromosomeassociated protein H, hCAP-H, and XCAP-H homolog, is encoded by a gene that is a member of the barr gene family. Brnn1 is the regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex contains the SMC2L1 and SMC4L1 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. BRRN1 has both a nuclear and cytoplasmic localization. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2 leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. BRRN1 is widely expressed at low level in most proliferating cells. CDC2 phosphorylates BRRN1. Its phosphorylation, as well as that of CNAP1 and CAPG subunits, activates the condensin complex and is required for chromosome condensation.

General readings

Torres-Rosell J, De Piccoli G, Cordon-Preciado V, Farmer S, Jarmuz A, Machin F, Pasero P, Lisby M, Haber JE, Aragon L. Anaphase onset before complete DNA replication with intact checkpoint responses. Science. 2007 Mar 9;315(5817):1411-5. PMID: 17347440

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCAPH antibody

TA303239 (0.01µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCAPH antibody

Western blot analysis of extracts from COLO cells, using NCAPH antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCAPH antibody

WB Suggested Anti-NCAPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysateNCAPH is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCAPH antibody

WB Suggested Anti-NCAPH Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate.NCAPH is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCAPH (440-457) antibody

NCAPH Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

NCAPH antibody

Western blot (WB) analysis of hCAP-H antibody (Cat.-No.: AP21157PU-N) in extracts from K562 cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NCAPH antibody

Western Blot analysis of NCAPH expression in transfected 293T cell line (H00023397-T01) by NCAPH MaxPab polyclonal antibody.

Lane 1: BRRN1 transfected lysate(81.51 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

NCAPH antibody

BRRN1 monoclonal antibody (M01), clone 1C9 Western Blot analysis of BRRN1 expression in Jurkat ( Cat # L017V1 ). Mouse IgG1 1C9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NCAPH antibody

NCAPH polyclonal antibody ( Cat # PAB7285 ) ( 0.01 µg/ml ) staining of K-562 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NCAPH antibody

Western blot analysis of extracts from COLO cells, using NCAPH Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

NCAPH antibody

Western blot analysis of extracts from COLO cells, using NCAPH Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

NCAPH antibody

Human Jurkat; Host:Rabbit. Target Name:NCAPH. Sample Tissue:Human Jurkat. Antibody Dilution: 1.0ug/ml. NCAPH is supported by BioGPS gene expression data to be expressed in Jurkat.; NCAPH antibody - N-terminal region (ARP46226_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NCAPH antibody

Human HepG2; WB Suggested Anti-NCAPH Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. NCAPH is supported by BioGPS gene expression data to be expressed in HepG2.; NCAPH antibody - C-terminal region (ARP46227_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NCAPH antibody

Western blot analysis of NCAPH expression in HeLa (A); Jurkat (B); MOLT4 (C) whole cell lysates. Rabbit Aff - Purified Hu WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NCAPH antibody

Western blot analysis of NCAPH expression in HeLa (A); Jurkat (B); MOLT4 (C) whole cell lysates. Rabbit Aff - Purified Hu WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NCAPH antibody

Western blot analysis of NCAPH expression in HeLa (A); Jurkat (B); MOLT4 (C) whole cell lysates. Rabbit Aff - Purified Hu WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

NCAPH antibody

Immunohistochemistry: NCAPH Antibody [NB100-60947] -  HCAP-H Antibody [NB100-60947] - NB100-60947 (0.01ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

NCAPH antibody

Immunohistochemistry: NCAPH Antibody [NBP1-32573] - analysis of paraffin-embedded SW480 xenograft, using BRRN1 antibody at 1:500 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

NCAPH antibody

NCAPH Rabbit Aff - Purified Hu WB
0.1 mg / €420.00
  Novus Biologicals Inc.

NCAPH antibody

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

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