NCAPH2 antibody

Principal name

NCAPH2 antibody

Alternative names for NCAPH2 antibody

CAPH2, Chromosome-associated protein H2

SwissProt ID

Q4V8I2 (Rat), Q6IBW4 (Human), Q8BSP2 (Mouse)

Gene ID

29781 (NCAPH2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of NCAPH2 antibody

Structural maintenance of chromosomes (SMC) and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. A crucial prerequisite for accurate segregation of replicated sister chromatids is the condensation of the chromosomes into a manageable form prior to metaphase. The condensin I complex consists of two SMC subunits, SMC2 and SMC4, and three non-SMC subunits, CAP-H, CAP-G, and CAP-D2. An alternative complex, the condensin II complex, contains alternate non-SMC subunits, CAP-G2, CAP-H2, and CAP-D3. CAP-H2 is also known as Non-SMC condensin II complex, subunit H2 (NCAPH2) or kleisin beta isoform 2. The three non-SMC subunits in the condensing complexes form a regulatory subcomplex that is required to activate the SMC ATPases and to promote mitosis-specific chromatin binding of the holocomplex. The precise individual functions of each non-SMC protein in activation remain to be determined.

General readings

Ono,T., (2003) Cell 115 (1), 109-121

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCAPH2 antibody

WB Suggested Anti-NCAPH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCAPH2 antibody

WB Suggested Anti-NCAPH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysateNCAPH2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCAPH2 (N-term) antibody

Western blot analysis of anti-NCAPH2 (AP11816PU-N) in HepG2 cell line lysate (35 µg/lane). NCAPH2 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NCAPH2 antibody

Western blot analysis of NCAPH2 polyclonal antibody ( Cat # PAB3814 ) in HepG2 cell line lysate ( 35 µg/lane ) . NCAPH2 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NCAPH2 antibody

Human HeLa; WB Suggested Anti-NCAPH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; NCAPH2 antibody - N-terminal region (ARP55483_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NCAPH2 antibody

Human 721_B; WB Suggested Anti-NCAPH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate. NCAPH2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; NCAPH2 antibody - N-terminal region (ARP55484_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NCAPH2 antibody

NCAPH2 Antibody [NBP1-28682] - Detection of Human CapH2 by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.   Antibodies: Affinity purified rabbit anti-CapH2 antibody A302-276A used for IP at 10 mcg/mg lysate.  CapH2 was also immunoprecipitated by rabbit anti-CapH2 antibody NBP1-28723, which recognizes an upstream epitope.  For blotting immunoprecipitated CapH2, NBP1-28723 was used at 1 mcg/ml, and the ReliaBLOT®  Reagents and Procedures were incorporated.  Detection: Chemiluminescence with an exposure time of 3 minutes. Rabbit IgG Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

NCAPH2 antibody

NCAPH2 Antibody [NBP1-28723] - Detection of Human CAP-H2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.   Antibodies: Affinity purified rabbit anti-CAP-H2 antibody NBP1-28723 used for WB at 1 mcg/ml (A and B) and used for IP at 10 mcg/mg lysate.  CAP-H2 was also immunoprecipitated by rabbit anti-CAP-H2 antibody NBP1-28682, which recognizes a downstream epitope.  For blotting immunoprecipitated CapH2, the ReliaBLOT®  Reagents and Procedures were used.  Detection: Chemiluminescence with exposure times of 3 minutes (A and B). Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

NCAPH2 antibody

Immunohistochemistry: NCAPH2 Antibody [NBP1-71956]-FFPE section of human skin carcinoma.  Antibody: Affinity purified rabbit anti-CAP-H2 used at a dilution of 1:250 Rabbit Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCAPH2 (transcript variant 1)

NCAPH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NCAPH2

NCAPH2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NCAPH2

NCAPH2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NCAPH2 overexpression lysate

NCAPH2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

NCAPH2 overexpression lysate

NCAPH2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

per page
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