NCF-4 antibody

Principal name

NCF-4 antibody

Alternative names for NCF-4 antibody

Neutrophil cytosol factor 4, SH3PXD4, p40-phox, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4

SwissProt ID

P97369 (Mouse), Q15080 (Human)

Gene ID

4689 (NCF4)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rb (Rabbit), Rt (Rat), Eq (Equine), Hst (Hamster), Insect, Por (Porcine), Prim (Primate), Bear, GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of NCF-4 antibody

NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

General readings

Eglinton,T.W., et al. (2012) Am. J. Gastroenterol. 107 (4), 589-596

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCF-4 antibody

  NCF4 mouse monoclonal antibody, clone OTI4B5 (formerly 4B5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NCF4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809135)(1:150) Mouse IgG1 OTI4B5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NCF-4 antibody

  Goat Polyclonal Antibody against NCF4 / P40PHOX  
TA302546 (0.5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCF-4 antibody

  Anti-NCF4 Rabbit Polyclonal Antibody  
Predicted band size: 39 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCF-4 antibody

  Rabbit Polyclonal Anti-NCF4 Antibody  
WB Suggested Anti-NCF4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCF-4 antibody

  Rabbit Polyclonal Anti-NCF4 Antibody  
WB Suggested Anti-NCF4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCF-4 antibody

Immunohistochemistry (IHC) analyzes of p40 phox antibody (Cat.-No.: AP06549PU-N) in paraffin-embedded human ovary tissue. Rabbit Aff - Purified Hu, Ms P
0.1 mg / €350.00
  Acris Antibodies GmbH

NCF-4 (228-238) antibody

Human Spleen (formalin-fixed, paraffin-embedded) stained with NCF4 antibody AP22576PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Can, Eq, Hst, Hu, Insect, Por, Prim E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCF-4 pThr154 antibody

Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €230.00
  Assay Biotech

NCF-4 pThr154 antibody

Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €330.00
  Assay Biotech

NCF-4 antibody

Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using p40 phox (Ab-154) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €230.00
  Assay Biotech

NCF-4 antibody

Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using p40 phox (Ab-154) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

NCF4 - human gene knockout kit via CRISPR

NCF4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NCF4 - human gene knockout kit via CRISPR

NCF4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NCF4 - human gene knockout kit via CRISPR

NCF4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NCF4 - human gene knockout kit via CRISPR

NCF4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCF-4 (transcript variant 1)

  Recombinant protein of human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1  
NCF-4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NCF-4 (full length, N-term HIS tag, transcript variant 2)

  Recombinant protein of human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
NCF-4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

NCF-4 (full length, N-term HIS tag, transcript variant 1)

  Purified recombinant protein of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
NCF-4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

NCF-4 (1-339, His-tag)

NCF-4 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

NCF-4 (1-339, His-tag)

NCF-4 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

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