NCF-4 antibody

Principal name

NCF-4 antibody

Alternative names for NCF-4 antibody

Neutrophil cytosol factor 4, SH3PXD4, p40-phox, Neutrophil NADPH oxidase factor 4, SH3 and PX domain-containing protein 4

SwissProt ID

P97369 (Mouse), Q15080 (Human)

Gene ID

4689 (NCF4), 6146890 (aceK)

Available reactivities

Hu (Human), Rb (Rabbit), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Frozen Sections (C)

Background of NCF-4 antibody

NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

General readings

Rioux JD, Xavier RJ, Taylor KD, Silverberg MS, Goyette P, Huett A, Green T, Kuballa P, Barmada MM, Datta LW, Shugart YY, Griffiths AM, Targan SR, Ippoliti AF, Bernard EJ, Mei L, Nicolae DL, Regueiro M, Schumm LP, Steinhart AH, Rotter JI, Duerr RH, Cho JH, Daly MJ, Brant SR. Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. Nat Genet. 2007 May;39(5):596-604. Epub 2007 Apr 15. PMID: 17435756

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCF-4 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NCF4 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809135)(1:150) Mouse IgG1 OTI4B5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NCF-4 antibody

TA302546 (0.5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCF-4 antibody

Predicted band size: 39 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCF-4 antibody

WB Suggested Anti-NCF4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCF-4 antibody

WB Suggested Anti-NCF4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCF-4 antibody

Immunohistochemistry (IHC) analyzes of p40 phox antibody (Cat.-No.: AP06549PU-N) in paraffin-embedded human ovary tissue. Rabbit Aff - Purified Hu, Ms P
0.1 mg / €350.00
  Acris Antibodies GmbH

NCF-4 (228-238) antibody

Human Spleen (formalin-fixed, paraffin-embedded) stained with NCF4 antibody AP22576PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Can, Eq, Hst, Hu, Insect, Por, Prim E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCF-4 antibody

NCF-4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NCF-4 antibody

NCF4 MaxPab polyclonal antibody. Western Blot analysis of NCF4 expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

NCF-4 antibody

Western Blot analysis of NCF4 expression in transfected 293T cell line (H00004689-T02) by NCF4 MaxPab polyclonal antibody.

Lane 1: NCF4 transfected lysate(39.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NCF-4 antibody

Detection limit for recombinant GST tagged NCF4 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 3C10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

NCF-4 antibody

293 overexpressing NCF4 and probed with NCF4 polyclonal antibody ( Cat # PAB7315 ) ( mock transfection in first lane), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NCF-4 pThr154 antibody

Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

NCF-4 pThr154 antibody

Immunohistochemistry analysis of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

NCF-4 antibody

Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using p40 phox (Ab-154) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

NCF-4 antibody

Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using p40 phox (Ab-154) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

NCF-4 antibody

Human Thymus; WB Suggested Anti-NCF4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Thymus; NCF4 antibody - N-terminal region (ARP46009_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NCF-4 antibody

Human HepG2; WB Suggested Anti-NCF4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; NCF4 antibody - middle region (ARP46010_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NCF-4 (C-term) antibody

NCF-4 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

NCF-4 antibody

Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666] - (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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