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NCF1 antibody

Principal name

NCF1 antibody

Alternative names for NCF1 antibody

NCF-1, Neutrophil cytosol factor 1, NOXO2, Nox organizer 2, SH3PXD1A, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

SwissProt ID

O77774 (Bovin), P14598 (Human), Q09014 (Mouse)

Gene ID

653361 (NCF1), 17969 (Ncf1)

Ncbi ID

9606, NP_000256, NP_000256.3, NP_035006.3, ABM84076.1, AAH02816, NM_000265, NM_010876

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit), Can (Canine), Por (Porcine)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NCF1 antibody

The phagocytic DPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The DPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of DPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformatiol change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558. Defects in p47phox, often resulting from recombition between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease. Elevated oxidative stress due to increased myocardial DPH oxidase activity may be a contributing factor in heart failure.

General readings

Wienties FB., et al. (2001) Biochem Biophys Res Commun. 289(2):382-8.
Doerries C., et al. (2007) Circ Res. 100:894-903.

NCF1 polyclonal antibody ( Cat # PAB6021 ) staining ( 0.2 µg/ml ) of U-937 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PAB6021

Immunohistochemistry (IHC) analyzes of p47-phox antibody (Cat.-No.: AP01461PU-N) in paraffin-embedded human testis tissue.

AP01461PU-N

Western blot (WB) analysis of p47-phox antibody (Cat.-No.: AP01461PU-N) in extracts from MCF7 (Lane 1) and HeLa (Lane 2) cells at 1/500 dilution.

AP01461PU-N

Colon, neutrophils: Formalin-Fixed Paraffin-Embedded (FFPE)

AP08603PU-N

Staining (AP08603PU-N at 0.2 µg/ml) of U937 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by Chemiluminescence.

AP08603PU-N

Recombinant human NCF1, 1- 390 aa, His-tagged

Immunohistochemistry analyzes of p47-phox antibody (AP20365PU-N) in paraffin-embedded human colon carcinoma tissue.

AP20365PU-N

Western blot analysis of p47-phox antibody (AP20365PU-N) in extracts from COS7 cells.

AP20365PU-N

Western blot analysis of extracts from COS7 cells treated with UV 15' and A549 cells, using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human tonsil, using p47 phox (Phospho-Ser370) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells treated with nocodazole 1ug/ml 18h, using p47 phox (Phospho-Ser359) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using p47 phox (Phospho-Ser345) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HepG2 cells treated with TNF 20ng/ml 5', using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using p47 phox (Ab-304) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COLO205, using p47 phox (Ab-304) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using p47 phox (Ab-328) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using p47 phox (Ab-328) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using p47 phox (Ab-370) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HT-29/COLO205/HepG2 cells, , using p47 phox (Ab-370) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using p47 phox (Ab-359) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, treated with TSA 400ng/ml 24h, using p47 phox (Ab-359) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using p47 phox (Ab-345) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HepG2 cells, treated with TNF 20ng/ml 5', using p47 phox (Ab-345) Antibody. The lane on the right is treated with the synthesized peptide.

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31369PU-N

Western blot analysis of extracts from HT-29/COLO205/HepG2 cells, , using p47 phox Antibody. The lane on the right is treated with the synthesized peptide.

AP31369PU-N

Staining of Mouse Thymus lysate using NCF1 antibody Cat.-No AP32141PU-N at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP32141PU-N

Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody AP55731PU-N. The lane on the left is treated with antigen-specific peptide.

Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody AP55828PU-N (left)or the same antibody preincubated with blocking peptide (right).

Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody AP55828PU-N.The lane on the right is treated with the antigen-specific peptide.

Immunofluorescent analysis of p47 phox (pS370) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of p47 phox (pS370) expression in HeLa serum starvation-treated (A); THP1 (B); Raw264.7 serum starvation-treated (C); H9C2 serum starvation-treated (D) whole cell lysates.

Immunohistochemical analysis of p47 phox staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of p47 phox expression in MCF7 (A); HeLa (B) whole cell lysates.

TP301233

TA302677 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TA302677

Western blot analysis of Raji cell lysates with anti-p47phox antibody (1:200).

TA310072

Predicted band size: 45 kDa . Positive control: T470 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321099

Western blot analysis of p47 phox expression in TSA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325708

Western blot analysis of p47 phox phosphorylation expression in nocodazole treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325709

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP32141PU-N	NCF1 antibody
	Goat		Aff - Purified	Bov, Can, Hu, Ms, Rt	WB	0.1 mg / €350.00
						Acris Antibodies GmbH
	Acris recommends this product for Western blotting.
TA302677	NCF1 (C-term) antibody
	Goat Polyclonal Antibody against NCF1 / p47phox
	Goat		Aff - Purified	Bov, Hu, Ms, Por, Rb, Rt	WB	0.1 mg / €325.00
						OriGene Technologies, Inc.
TA310072	NCF1 antibody
	Rabbit anti-NCF1 polyclonal antibody
	Rabbit		Purified	Ms, Rt	E, WB	0.1 ml / €325.00
						OriGene Technologies, Inc.
TA321099	NCF1 antibody
	Anti-NCF1 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 ml / €325.00
						OriGene Technologies, Inc.
TA325708	NCF1 antibody
	Rabbit Polyclonal p47 phox Antibody
	Rabbit	IgG	Purified	Hu	WB	0.1 ml / €325.00
						OriGene Technologies, Inc.
TA325709	NCF1 antibody
	Rabbit Polyclonal p47 phox (Ser359) Antibody (Phospho-specific)
	Rabbit	IgG	Purified	Hu	WB	0.1 ml / €325.00
						OriGene Technologies, Inc.
AP01461PU-N	NCF1 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €350.00
						Acris Antibodies GmbH
+1 additional image
AP08603PU-N	NCF1 (C-term) antibody
	Goat	IgG	Aff - Purified	Bov, Hu, Mky	E, P, WB	50 µg / €470.00
						Acris Antibodies GmbH
+1 additional image
AP20365PU-N	NCF1 antibody
	Rabbit		Aff - Purified	Hu	ICC/IF, P, WB	0.1 mg / €350.00
						Acris Antibodies GmbH
+1 additional image
AP31369PU-N	NCF1 (C-term) antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	50 µl / €470.00
						Acris Antibodies GmbH
+1 additional image
AP55731PU-N	NCF1 pSer304 antibody
	Rabbit		Aff - Purified	Hu	WB	0.1 ml / €340.00
						Acris Antibodies GmbH
AP55731PU-S	NCF1 pSer304 antibody
	Rabbit		Aff - Purified	Hu	WB	50 µl / €270.00
						Acris Antibodies GmbH
AP55828PU-N	NCF1 pSer345 antibody
	Rabbit		Aff - Purified	Hu	P, WB	0.1 ml / €340.00
						Acris Antibodies GmbH
+1 additional image
AP55828PU-S	NCF1 pSer345 antibody
	Rabbit		Aff - Purified	Hu	P, WB	50 µl / €270.00
						Acris Antibodies GmbH
+1 additional image
A1160-1	NCF1 pSer304 antibody
	Rabbit		Aff - Purified	Hu	E, WB	50 µg / €230.00
						Assay Biotech
A1160-2	NCF1 pSer304 antibody
	Rabbit		Aff - Purified	Hu	E, WB	0.1 mg / €330.00
						Assay Biotech
A1161-1	NCF1 pSer328 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	E, P	50 µg / €230.00
						Assay Biotech
A1161-2	NCF1 pSer328 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	E, P	0.1 mg / €330.00
						Assay Biotech
A1171-1	NCF1 pSer370 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	E, P	50 µg / €230.00
						Assay Biotech
A1171-2	NCF1 pSer370 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	E, P	0.1 mg / €330.00
						Assay Biotech