NCF1 antibody

Principal name

NCF1 antibody

Alternative names for NCF1 antibody

NCF-1, Neutrophil cytosol factor 1, NOXO2, Nox organizer 2, SH3PXD1A, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

SwissProt ID

O77774 (Bovin), P14598 (Human), Q09014 (Mouse)

Gene ID

653361 (NCF1), 17969 (Ncf1)

Available reactivities

Bov (Bovine), Do (Dolphin), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Por (Porcine), Can (Canine)

Available hosts

Goat, Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NCF1 antibody

The phagocytic DPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The DPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of DPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformatiol change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558. Defects in p47phox, often resulting from recombition between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease. Elevated oxidative stress due to increased myocardial DPH oxidase activity may be a contributing factor in heart failure.

General readings

Davidson-Moncada JK et al. Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem J 363:95-103 (2002). PubMed PMID: 11903052

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCF1 (C-term) antibody

TA302677 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rb, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH

NCF1 antibody

Staining of Mouse Thymus lysate using NCF1 antibody Cat.-No AP32141PU-N at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

NCF1 (C-term) antibody

TA302677 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCF1 antibody

Western blot analysis of Raji cell lysates with anti-p47phox antibody (1:200). Rabbit Purified Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCF1 antibody

Predicted band size: 45 kDa . Positive control: T470 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCF1 antibody

Western blot analysis of p47 phox expression in TSA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCF1 antibody

Immunohistochemistry (IHC) analyzes of p47-phox antibody (Cat.-No.: AP01461PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NCF1 (C-term) antibody

Colon, neutrophils: Formalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bov, Hu, Ms, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCF1 antibody

Immunohistochemistry analyzes of p47-phox antibody (AP20365PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NCF1 (C-term) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCF1 pSer304 antibody

Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody AP55731PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu WB
0.1 ml / €340.00
  Acris Antibodies GmbH

NCF1 pSer304 antibody

Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody AP55731PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu WB
50 µl / €270.00
  Acris Antibodies GmbH

NCF1 pSer345 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody AP55828PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

NCF1 pSer345 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody AP55828PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P, WB
50 µl / €270.00
  Acris Antibodies GmbH

NCF1 antibody

NCF1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

NCF1 antibody

NCF1 polyclonal antibody ( Cat # PAB6021 ) staining ( 0.2 µg/ml ) of U-937 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NCF1 pSer304 antibody

Western blot analysis of extracts from COS7 cells treated with UV 15' and A549 cells, using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

NCF1 pSer304 antibody

Western blot analysis of extracts from COS7 cells treated with UV 15' and A549 cells, using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

NCF1 pSer328 antibody

Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

NCF1 pSer328 antibody

Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

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