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NCF1 antibody

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Principal name

NCF1 antibody

Alternative names for NCF1 antibody

NCF-1, Neutrophil cytosol factor 1, NOXO2, Nox organizer 2, SH3PXD1A, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

SwissProt ID

O77774 (Bovin), P14598 (Human), Q09014 (Mouse)

Gene ID

653361 (NCF1), 17969 (Ncf1)

Ncbi ID

NP_000256.3, 9606, NP_000256, NP_035006.3, ABM84076.1, AAH02816, NM_000265

Available reactivities

Bov (Bovine), Do (Dolphin), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Por (Porcine), Can (Canine)

Available hosts

Goat, Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NCF1 antibody

The phagocytic DPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The DPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of DPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformatiol change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558. Defects in p47phox, often resulting from recombition between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease. Elevated oxidative stress due to increased myocardial DPH oxidase activity may be a contributing factor in heart failure.

General readings

Davidson-Moncada JK et al. Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem J 363:95-103 (2002). PubMed PMID: 11903052

GTX20795 - NCF1
GTX20795 staining (0.2g/ml) of U937 lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
NB100-790 - NCF1
NB 100-790 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
PAB6021 - NCF1
NCF1 polyclonal antibody ( Cat # PAB6021 ) staining ( 0.2 µg/ml ) of U-937 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
AP01461PU-N - NCF1
Immunohistochemistry (IHC) analyzes of p47-phox antibody (Cat.-No.: AP01461PU-N) in paraffin-embedded human testis tissue.
AP01461PU-N - NCF1
Western blot (WB) analysis of p47-phox antibody (Cat.-No.: AP01461PU-N) in extracts from MCF7 (Lane 1) and HeLa (Lane 2) cells at 1/500 dilution.
AP15882PU-N - NCF1
AP15882PU-N NCF1 antibody staining of U937 lysate at 0.2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP08603PU-N - NCF1
Colon, neutrophils: Formalin-Fixed Paraffin-Embedded (FFPE)
AP08603PU-N - NCF1
Staining (AP08603PU-N at 0.2 µg/ml) of U937 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by Chemiluminescence.
NBL1-13509 - NCF1 Lysate
Western Blot: NCF1 Lysate [NBL1-13509] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for NCF1
AR09591PU-L - NCF1
AR09591PU-L - NCF1
Recombinant human NCF1, 1- 390 aa, His-tagged
AP20365PU-N - NCF1
Immunohistochemistry analyzes of p47-phox antibody (AP20365PU-N) in paraffin-embedded human colon carcinoma tissue.
AP20365PU-N - NCF1
Western blot analysis of p47-phox antibody (AP20365PU-N) in extracts from COS7 cells.
A1160-1 - NCF1
Western blot analysis of extracts from COS7 cells treated with UV 15' and A549 cells, using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the right is treated with the synthesized peptide.
A1161-1 - NCF1
Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody. The picture on the right is treated with the synthesized peptide.
A1171-1 - NCF1
Immunohistochemistry analysis of paraffin-embedded human tonsil, using p47 phox (Phospho-Ser370) Antibody. The picture on the right is treated with the synthesized peptide.
A1172-1 - NCF1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is treated with the synthesized peptide.
A1172-1 - NCF1
Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is treated with the synthesized peptide.
A1172-1 - NCF1
Western blot analysis of extracts from HeLa cells treated with nocodazole 1ug/ml 18h, using p47 phox (Phospho-Ser359) Antibody. The lane on the right is treated with the synthesized peptide.
A8391-1 - NCF1
Immunohistochemistry analysis of paraffin-embedded human brain, using p47 phox (Phospho-Ser345) Antibody. The picture on the right is treated with the synthesized peptide.
A8391-1 - NCF1
Western blot analysis of extracts from HepG2 cells treated with TNF 20ng/ml 5', using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the synthesized peptide.
B1160-1 - NCF1
Immunofluorescence analysis of HeLa cells, using p47 phox (Ab-304) Antibody. The picture on the right is treated with the synthesized peptide.
B1160-1 - NCF1
Western blot analysis of extracts from COLO205, using p47 phox (Ab-304) Antibody. The lane on the right is treated with the synthesized peptide.
B1161-1 - NCF1
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using p47 phox (Ab-328) Antibody. The picture on the right is treated with the synthesized peptide.
B1161-1 - NCF1
Immunofluorescence analysis of NIH-3T3 cells, using p47 phox (Ab-328) Antibody. The picture on the right is treated with the synthesized peptide.
B1171-1 - NCF1
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using p47 phox (Ab-370) Antibody. The picture on the right is treated with the synthesized peptide.
B1171-1 - NCF1
Western blot analysis of extracts from HT-29/COLO205/HepG2 cells, , using p47 phox (Ab-370) Antibody. The lane on the right is treated with the synthesized peptide.
B1172-1 - NCF1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using p47 phox (Ab-359) Antibody. The picture on the right is treated with the synthesized peptide.
B1172-1 - NCF1
Western blot analysis of extracts from HeLa cells, treated with TSA 400ng/ml 24h, using p47 phox (Ab-359) Antibody. The lane on the right is treated with the synthesized peptide.
B8391-1 - NCF1
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using p47 phox (Ab-345) Antibody. The picture on the right is treated with the synthesized peptide.
B8391-1 - NCF1
Western blot analysis of extracts from HepG2 cells, treated with TNF 20ng/ml 5', using p47 phox (Ab-345) Antibody. The lane on the right is treated with the synthesized peptide.
AP31369PU-N - NCF1
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP31369PU-N - NCF1
Western blot analysis of extracts from HT-29/COLO205/HepG2 cells, , using p47 phox Antibody. The lane on the right is treated with the synthesized peptide.
GTX118361 - NCF1
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE GTX118361 diluted at 1:5000
GTX118361 - NCF1
Sample (50 ug of whole cell lysate) A: mouse spleen 10% SDS PAGE GTX118361 diluted at 1:500
GTX102957 - NCF1
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCF1(GTX102957) antibody at 1:500 dilution.
GTX102957 - NCF1
NCF1 antibody detects NCF1 protein at cytoplasm by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NCF1 protein stained by NCF1 antibody (GTX102957) diluted at 1:500.Blue: Hoechst 33342 staining.
GTX102957 - NCF1
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE GTX102957 diluted at 1:5000
AP32141PU-N - NCF1
Staining of Mouse Thymus lysate using NCF1 antibody Cat.-No AP32141PU-N at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GTX89999 - NCF1
GTX89999 (0.5µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
bs-3886R - NCF1
Formalin-fixed and human endometrium tissue labeled with Anti-NCF1/p47 phox Polyclonal Antibody, Unconjugated (bs-3886R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
bs-3886R - NCF1
Lane 1: human colon carcinoma lysates Lane 2: mouse brain lysates probed with Anti NCF1/p47 phox Polyclonal Antibody, Unconjugated (bs-3886R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 45kD. Observed band size: 45kD
bs-6966R - NCF1
Formalin-fixed and paraffin embedded rat brain labeled with Anti p47-phox Polyclonal Antibody, Unconjugated (bs-6966R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
AP55731PU-S - NCF1
Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody AP55731PU-N. The lane on the left is treated with antigen-specific peptide.
AP55828PU-S - NCF1
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody AP55828PU-N (left)or the same antibody preincubated with blocking peptide (right).
AP55828PU-S - NCF1
Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody AP55828PU-N.The lane on the right is treated with the antigen-specific peptide.
CPA2921-100ul - NCF1
Immunofluorescent analysis of p47 phox (pS370) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2921-100ul - NCF1
Western blot analysis of p47 phox (pS370) expression in HeLa serum starvation-treated (A); THP1 (B); Raw264.7 serum starvation-treated (C); H9C2 serum starvation-treated (D) whole cell lysates.
CPA2922-100ul - NCF1
Immunohistochemical analysis of p47 phox staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2922-100ul - NCF1
Western blot analysis of p47 phox expression in MCF7 (A); HeLa (B) whole cell lysates.
TP301233 - NCF1

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCF1 (C-term) antibody
TA302677 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rb, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH
 

Acris recommends this product for Western blotting.

NCF1 antibody
Staining of Mouse Thymus lysate using NCF1 antibody Cat.-No AP32141PU-N at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH
 

Acris recommends this product for Western blotting.

NCF1 (C-term) antibody
TA302677 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCF1 antibody
Western blot analysis of Raji cell lysates with anti-p47phox antibody (1:200). Rabbit Purified Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCF1 antibody
Predicted band size: 45 kDa . Positive control: T470 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCF1 antibody
Western blot analysis of p47 phox expression in TSA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCF1 antibody
Immunohistochemistry (IHC) analyzes of p47-phox antibody (Cat.-No.: AP01461PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

NCF1 (C-term) antibody
Colon, neutrophils: Formalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bov, Hu, Ms, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH
+1 additional image

NCF1 antibody
Immunohistochemistry analyzes of p47-phox antibody (AP20365PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

NCF1 (C-term) antibody
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH
+1 additional image

NCF1 pSer304 antibody
Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody AP55731PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu WB
0.1 ml / €340.00
  Acris Antibodies GmbH

NCF1 pSer304 antibody
Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody AP55731PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu WB
50 µl / €270.00
  Acris Antibodies GmbH

NCF1 pSer345 antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody AP55828PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P, WB
0.1 ml / €340.00
  Acris Antibodies GmbH
+1 additional image

NCF1 pSer345 antibody
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody AP55828PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu P, WB
50 µl / €270.00
  Acris Antibodies GmbH
+1 additional image

NCF1 antibody
NCF1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

NCF1 antibody
NCF1 polyclonal antibody ( Cat # PAB6021 ) staining ( 0.2 µg/ml ) of U-937 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NCF1 pSer304 antibody
Western blot analysis of extracts from COS7 cells treated with UV 15' and A549 cells, using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

NCF1 pSer304 antibody
Western blot analysis of extracts from COS7 cells treated with UV 15' and A549 cells, using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

NCF1 pSer328 antibody
Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

NCF1 pSer328 antibody
Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

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53 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (48)
  3. Protein | Growth Factor (4)
Clonality
  1. Polyclonal (48)
Target
  1. NCF1 (52)
Property
  1. C-term (4)
  2. pSer304 (4)
  3. pSer328 (2)
  4. pSer345 (4)
  5. pSer359 (2)
  6. pSer370 (5)
Reactivity
  1. Human (Hu) (47)
  2. Mouse (Ms) (24)
  3. Rat (Rt) (24)
  4. Rabbit (Rb) (9)
  5. Bovine (Bov) (4)
  6. Porcine (Por) (2)
  7. Canine (Can) (1)
Applications
  1. Western blot / Immunoblot (WB) (40)
  2. Paraffin Sections (P) (30)
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Images
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  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (36)
  2. Paraffin Sections (P) (25)
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Species (Protein)
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Source
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  • fax: +49-5221-34606-11
  • email: info@acris-online.de
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