NCK1 antibody

Principal name

NCK1 antibody

Alternative names for NCK1 antibody

Cytoplasmic protein NCK1, NCK, NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha

SwissProt ID

P16333 (Human)

Gene ID

4690 (NCK1), 4690370 (IPR002582)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of NCK1 antibody

Nck is a highly conserved, oncogenic protein. It is a common target for the action of different surface receptors, encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase activating protein, phosphatidylinositol 3 kinase, and phospholipase Cg. Nck is widely expressed in various tissues and in cell lines from human, murine, and rat origins. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively. Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells.

General readings

Braverman. L.E. and Quilliam. L.A. (1999) J Biol Chem 274: 5542-5549 Latreille. M. and Larose. L. (2006) J Biol Chem 281: 26633-26644 Kremer. B.E., et al. (2007) Cell 130: 837-850

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCK1 antibody

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining. Mouse IgG1 5B7 Purified Hu, Mky E, F, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NCK1 antibody

Predicted band size: 43 kDa . Positive control: Jurkat and K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCK1 antibody

Predicted band size: 43 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCK1 (2nd SH3 dom.) antibody

Western blot analysis of NCK1 in whole cell lysate of mouse lymph node lymphocytes using the antibody EM-06. Mouse IgG1 EM-06 Purified Hu, Ms ICC/IF, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

NCK1 antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCK1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 AT6B3 Purified Hu E, F, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

NCK1 antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCK1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 AT6B3 Purified Hu E, F, WB
50 µl / €220.00
  Acris Antibodies GmbH

NCK1 (N-term) antibody

Western blot analysis of NCK1 Antibody (N-term) (AP18125PU-N) in Hela cell line lysates (35ug/lane). NCK1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NCK1 antibody

NCK1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NCK1 antibody

Immunofluorescence of purified MaxPab antibody to NCK1 on HeLa cell. [antibody concentration 20 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

NCK1 antibody

NCK1 Mouse Hu ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

NCK1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with anti-NCK1 rabbit purified polyclonal 1:1200 and anti-ABL2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NCK1 antibody

NCK1 monoclonal antibody (M01), clone 1A1 Western Blot analysis of NCK1 expression in HeLa NE ( Cat # L013V3 ). Mouse IgG 1A1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NCK1 antibody

Immunoblot analysis of cell lysates.
Lane 1 : HeLa cell lysate.
 Lnae 2 : RD cell lysate.
Lane 3 : PC-12 cell lysate.
Lane 4 : NIH/3T3 cell lysate. Mouse 11D10 Hu, Ms, Rt E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

NCK1 antibody

NCK1 Rabbit Hu, Ms WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

NCK1 antibody

NCK1 Rabbit Hu E, IP, P, WB
50 µg / €440.00
  Novus Biologicals Inc.

NCK1 antibody

Immunofluorescent: Nck Antibody (Y531) [NB110-57240] - Immunofluorescent staining of HeLa cells using anti- NAK/TBK1 (N-term) (cat. # 110-57240). Rabbit IgG Y531 Aff - Purified Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

NCK1 / NCK2 antibody

Immunoblot analysis of cell lysates.
Lane 1 : U937 cell lysate.
 Lnae 2 : A-431 cell lysate.
Lane 3 : NIH/3H3 cell lysate.
Lane 4 : C6 cell lysate. Rabbit IgG Hu, Ms, Rt WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCK1 (1-377, His-tag)

NCK1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

NCK1 (1-377, His-tag)

NCK1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

NCK1

NCK1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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