NCK1 antibody

Principal name

NCK1 antibody

Alternative names for NCK1 antibody

Cytoplasmic protein NCK1, NCK, NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha

SwissProt ID

P16333 (Human)

Gene ID

4690 (NCK1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of NCK1 antibody

Kit Component:
- KN202869G1, NCK1 gRNA vector 1 in pCas-Guide vector
- KN202869G2, NCK1 gRNA vector 2 in pCas-Guide vector
- KN202869D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Braverman. L.E. and Quilliam. L.A. (1999) J Biol Chem 274: 5542-5549 Latreille. M. and Larose. L. (2006) J Biol Chem 281: 26633-26644 Kremer. B.E., et al. (2007) Cell 130: 837-850

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCK1 antibody

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining. Mouse IgG1 5B7 Purified Hu, Mky E, F, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NCK1 antibody

Predicted band size: 43 kDa . Positive control: Jurkat and K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCK1 antibody

Predicted band size: 43 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCK1 (2nd SH3 dom.) antibody

Western blot analysis of NCK1 in whole cell lysate of mouse lymph node lymphocytes using the antibody EM-06. Mouse IgG1 EM-06 Purified Hu, Ms ICC/IF, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

NCK1 antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCK1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 AT6B3 Purified Hu E, F, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

NCK1 antibody

The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCK1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG3 AT6B3 Purified Hu E, F, WB
50 µl / €220.00
  Acris Antibodies GmbH

NCK1 (N-term) antibody

Western blot analysis of NCK1 Antibody (N-term) (AP18125PU-N) in Hela cell line lysates (35ug/lane). NCK1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NCK1 antibody

NCK1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NCK1 antibody

Immunofluorescence of purified MaxPab antibody to NCK1 on HeLa cell. [antibody concentration 20 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

NCK1 antibody

NCK1 Mouse Hu ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

NCK1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with anti-NCK1 rabbit purified polyclonal 1:1200 and anti-ABL2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NCK1 antibody

NCK1 monoclonal antibody (M01), clone 1A1 Western Blot analysis of NCK1 expression in HeLa NE ( Cat # L013V3 ). Mouse IgG 1A1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NCK1 antibody

Immunoblot analysis of cell lysates.
Lane 1 : HeLa cell lysate.
 Lnae 2 : RD cell lysate.
Lane 3 : PC-12 cell lysate.
Lane 4 : NIH/3T3 cell lysate. Mouse 11D10 Hu, Ms, Rt E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

NCK1 antibody

NCK1 Rabbit Hu, Ms WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

NCK1 / NCK2 antibody

Immunoblot analysis of cell lysates.
Lane 1 : U937 cell lysate.
 Lnae 2 : A-431 cell lysate.
Lane 3 : NIH/3H3 cell lysate.
Lane 4 : C6 cell lysate. Rabbit IgG Hu, Ms, Rt WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCK1 (1-377, His-tag)

NCK1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

NCK1 (1-377, His-tag)

NCK1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

NCK1

NCK1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NCK1

NCK1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

NCK1

NCK1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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