NCOA1 antibody

Principal name

NCOA1 antibody

Alternative names for NCOA1 antibody

NCoA-1, Nuclear receptor coactivator 1, Steroid receptor coactivator 1, SRC-1, RIP160, Protein Hin-2, NY-REN-52, BHLHE74, Class E basic helix-loop-helix protein 74

SwissProt ID

P70365 (Mouse), Q15788 (Human), Q4PJW2 (Pig), Q6GVI5 (Human)

Gene ID

8648 (NCOA1)

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Prim (Primate), Av (Birds), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), PLA/Proximity ligation assay (PLA), Frozen Sections (C), ChIP assay (ChIP), Flow Cytometry (F)

Background of NCOA1 antibody

There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptiol activators such as Stat3, NF-?B, E2F1, and p53). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases. In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes. The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection). SRC-1 and SRC-3 are conduits for kise-mediated growth factor sigling to the estrogen receptor and other transcriptiol activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kise sigling pathways). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers, and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively.

General readings

Bailey, S.D., et al. Diabetes Care 33(10): 2250-2253 (2010)
Son, Y.L., et al. FEBS Lett. 584(18): 3862-3866 (2010)
McIlroy, M., et al. Cancer Res. 70(4): 1585-1594 (2010)
Hartmaier, R.J., et al. BMC Cancer 9: 438 (2009)

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCOA1 (N-term) antibody

Western blot analysis of SRC1 (arrow) using rabbit polyclonal SRC1 Antibody (N-term) (Cat.#TA302235). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SRC1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCOA1 antibody

Predicted band size: 157 kDa . Positive control: Testis tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCOA1 antibody

WB Suggested Anti-NCOA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: NCI-H226 cell lysate Rabbit IgG Purified Bov, Eq, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCOA1 (477-947) antibody

AM06051PU-N NCOA1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cerebellum at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 1135 Purified Hu, Mky, Ms, Rt IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCOA1 (Center) antibody

Western blot analysis in MDA-MB231 cell line lysates (35ug/lane) using NCOA1 / SRC1 antibody Cat.-No. AP54040PU-N. This demonstrates the NCOA1 antibody detected the NCOA1protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NCOA1 antibody

NCOA1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NCOA1 antibody

Western blot analysis of SRC1 ( arrow ) using rabbit NCOA1 polyclonal antibody ( Cat # PAB2970 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NCOA1 gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NCOA1 antibody

Human NCI-H226; WB Suggested Anti-NCOA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: NCI-H226 cell lysate; NCOA1 antibody - middle region (ARP33991_P050) in Human NCI-H226 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NCOA1 antibody

Immunoprecipitation: SRC1 Antibody [NB100-312] Nuclear extract (10 mg) from HeLa cells. Affinity purified rabbit anti-SRC1 used at 20 mcg/10 mg extract. Rabbit Aff - Purified Hu IP, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NCOA1 antibody

Immunoprecipitation: SRC1 Antibody [NB100-313] Nuclear extract (10 mg) from HeLa cells.  Affinity purified rabbit anti-SRC1 used at 20 mcg/10 mg extract. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NCOA1 antibody

NCOA1 Mouse 1135 Purified Hu IP, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCOA1

NCOA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NCOA1

NCOA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NCOA1 overexpression lysate

NCOA1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

NCOA1 overexpression lysate

NCOA1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

NCOA1 overexpression lysate

NCOA1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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