NCOA2 antibody

Principal name

NCOA2 antibody

Alternative names for NCOA2 antibody

NCoA-2, Nuclear receptor coactivator 2, TIF2, GRIP1, SRC-2, BHLHE75, Transcriptional intermediary factor 2, Glucocorticoid receptor-interacting protein-1, Class E basic helix-loop-helix protein 75

SwissProt ID

Q15596 (Human), Q61026 (Mouse), Q98TW1 (Danre), Q9W705 (Xenla), Q9WUI9 (Rat)

Gene ID

10499 (NCOA2), 1210499 (mutM1)

Available reactivities

Hu (Human), Ms (Mouse), Xen (Xenopus), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NCOA2 antibody

The nuclear receptor transcriptional activity is regulated by coregulators including coactivators (SRC-1, SRC-2 and SRC-3, RIP140 etc.) and co-repressors (HDAC1, HDAC2 and HDAC3, SMRT and NoCRs). The transactivational potency of agonist bound progesterone receptor (PR) can be significantly enhanced by increasing the cellular level of members of the steroid receptor coactivator (SRC/p160) family of coregulators. The coactivators of NF-kB include P300/CBP, p/CAF, and p160 proteins (SRC-1, SRC-2 and SRC-3). CBP/p300 and the p160 (HDACs) proteins both possess intrinsic histone acetylase (HAT) activity, which is necessary to open the chromatin structure through an acetylation-induced conformation change in histone protein. The coactivators of nuclear receptors provide an bridging apparatus between nuclear receptor and the transcription machinery. It has recently been shown that Orphan receptor SF-1, which regulates the cAMP stimulated expression of human steroidenic acute regulatory protein gene DAX-1, interacts with RIP140. The steroid receptor co activator 2 (SRC-2) knockout mice exhibited severe reproductive defects in both sexes, the males are hypofertile with developmental defect in spermatogenesis and age-dependent testicular degeneration. The females revealed a significant hypo-fertility phenotype die to partial placental hypoplacia (1). The components of the nuclear receptor NF-kB include SMRT, NCoR, HDAC1, HDAC2 and HDAC3, the SMRT and NCoR do not have an intrinsic enzyme activity , but they can trigger the catalytic activity of histone deacetylase (HDAC) for deacetylation of histone proteins (2). HDAC1–3 belongs to the class I histone deacetylases that includes HDAC1, 2, 3, 8, and 11 (3). The class II histone deacetylases include HDAC4, 5, 6, 7, 9, and 10. The catalytic activity of HDACs is required for deacetylation of histones and transcription factors in the regulation of transcription.
NuCR-2 is also known as GRIP-1 is involved in skeletal myogenic differentitation by interacting with basic helix-loop-helis (bHLH) and myogenic regulatory factors (MRFs) including MyoD, myogenin, Myf5 and MRF4 (4), and with class II receptor (TRa, VDR, RARa, RXRa) which show high degree of sequence homology with SRC-11 (4). NuCR2 is expressed in 2 isoforms a and b with MW in 160 kDa range and is distributed in both cytoplasm and nucleus, the distribution is regulated y cell differentiation. NuCR2 as two activation domains (AD1 and AD2)positioned in the C-terminal regionand are responsible for recruiting secondary regulators. The AD1 domain interacts with HDAC and related CRE-binding proteins, where as AD2 recruits Arginine methyl transferases such as co-activator-associated Arginine Transferase 1 (CARM1) (5).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCOA2 antibody

WB Suggested Anti-NCOA2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCOA2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NCOA2 (RC212235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCOA2. Mouse IgG1 OTI3C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NCOA2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NCOA2 (RC212235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCOA2. Mouse IgG1 OTI7G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NCOA2 antibody

Detection of Human SRC2 in Nuclear (A) or Cytosolic (S100) (B) extracts of Hela cells using AP05333PU-N SRC2 antibody at 20mcg/10mg lysate. Immunoprecipitate was resolved by SDS-PAGE and the identity of the SRC2 band verified by Mass Spectroscopy. Goat IgG Purified Hu, Ms IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

NCOA2 Control Peptide

NCOA2
0.25 mg / €250.00
  Acris Antibodies GmbH

NCOA2 (1379-1393) antibody

Western blot with AP10177PU-N (1:500 in dilution buffer). Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

NCOA2 pSer736 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NCoA2 (Phospho-Ser736) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
50 µg / €205.00
  Assay Biotech

NCOA2 pSer736 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NCoA2 (Phospho-Ser736) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €310.00
  Assay Biotech

NCOA2 antibody

NCOA2 Rabbit Aff - Purified Hu E, P
50 µg / €205.00
  Assay Biotech

NCOA2 antibody

NCOA2 Rabbit Aff - Purified Hu E, P
0.1 mg / €310.00
  Assay Biotech

NCOA2 antibody

Human Thymus; WB Suggested Anti-NCOA2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Thymus; NCOA2 antibody - N-terminal region (ARP38815_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NCOA2 antibody

NCOA2 Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

NCOA2 antibody

Western Blot and Immunoprecipitation: SRC2 Antibody [NB100-388] - Detection of Human SRC2 on HeLa whole cell lysate using NB100-388. Rabbit Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

NCOA2 antibody

Immunohistochemistry: SRC2 Antibody [NB100-1756] - Detection of Human SRC2 by Immunohistochemistry.  Samples:  FFPE sections of human breast adenocarcinoma (upper image) and prostate adenocarcinoma (lower image).  Antibody: Affinity purified rabbit anti-SRC2 NB100-1756 used at a dilution of 1:100.  Detection:  DAB Rabbit Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

NCOA2 antibody

NCOA2 Rabbit Agarose Hu IP
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCOA2

NCOA2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NCOA2

NCOA2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NCOA2 overexpression lysate

NCOA2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

18 Item(s)

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