NCOA2 antibody

Principal name

NCOA2 antibody

Alternative names for NCOA2 antibody

NCoA-2, Nuclear receptor coactivator 2, TIF2, GRIP1, SRC-2, BHLHE75, Transcriptional intermediary factor 2, Glucocorticoid receptor-interacting protein-1, Class E basic helix-loop-helix protein 75

SwissProt ID

Q15596 (Human), Q61026 (Mouse), Q98TW1 (Danre), Q9W705 (Xenla), Q9WUI9 (Rat)

Gene ID

10499 (NCOA2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of NCOA2 antibody

The nuclear receptor transcriptional activity is regulated by coregulators including coactivators (SRC-1, SRC-2 and SRC-3, RIP140 etc.) and co-repressors (HDAC1, HDAC2 and HDAC3, SMRT and NoCRs). The transactivational potency of agonist bound progesterone receptor (PR) can be significantly enhanced by increasing the cellular level of members of the steroid receptor coactivator (SRC/p160) family of coregulators. The coactivators of NF-kB include P300/CBP, p/CAF, and p160 proteins (SRC-1, SRC-2 and SRC-3). CBP/p300 and the p160 (HDACs) proteins both possess intrinsic histone acetylase (HAT) activity, which is necessary to open the chromatin structure through an acetylation-induced conformation change in histone protein. The coactivators of nuclear receptors provide an bridging apparatus between nuclear receptor and the transcription machinery. It has recently been shown that Orphan receptor SF-1, which regulates the cAMP stimulated expression of human steroidenic acute regulatory protein gene DAX-1, interacts with RIP140. The steroid receptor co activator 2 (SRC-2) knockout mice exhibited severe reproductive defects in both sexes, the males are hypofertile with developmental defect in spermatogenesis and age-dependent testicular degeneration. The females revealed a significant hypo-fertility phenotype die to partial placental hypoplacia (1). The components of the nuclear receptor NF-kB include SMRT, NCoR, HDAC1, HDAC2 and HDAC3, the SMRT and NCoR do not have an intrinsic enzyme activity , but they can trigger the catalytic activity of histone deacetylase (HDAC) for deacetylation of histone proteins (2). HDAC1–3 belongs to the class I histone deacetylases that includes HDAC1, 2, 3, 8, and 11 (3). The class II histone deacetylases include HDAC4, 5, 6, 7, 9, and 10. The catalytic activity of HDACs is required for deacetylation of histones and transcription factors in the regulation of transcription.
NuCR-2 is also known as GRIP-1 is involved in skeletal myogenic differentitation by interacting with basic helix-loop-helis (bHLH) and myogenic regulatory factors (MRFs) including MyoD, myogenin, Myf5 and MRF4 (4), and with class II receptor (TRa, VDR, RARa, RXRa) which show high degree of sequence homology with SRC-11 (4). NuCR2 is expressed in 2 isoforms a and b with MW in 160 kDa range and is distributed in both cytoplasm and nucleus, the distribution is regulated y cell differentiation. NuCR2 as two activation domains (AD1 and AD2)positioned in the C-terminal regionand are responsible for recruiting secondary regulators. The AD1 domain interacts with HDAC and related CRE-binding proteins, where as AD2 recruits Arginine methyl transferases such as co-activator-associated Arginine Transferase 1 (CARM1) (5).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCOA2 antibody

  Rabbit polyclonal NCoA2 (Ser736) antibody(Phospho-specific)  
Western blot analysis of extracts from HeLa cells, treated with TSA (400nM, 24hours), using NCoA2 (Phospho-Ser736) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCOA2 antibody

  Rabbit Polyclonal Anti-NCOA2 Antibody  
WB Suggested Anti-NCOA2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCOA2 antibody

  NCOA2 mouse monoclonal antibody, clone OTI3C7 (formerly 3C7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NCOA2 (RC212235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCOA2. Mouse IgG1 OTI3C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NCOA2 antibody

  NCOA2 mouse monoclonal antibody, clone OTI7G5 (formerly 7G5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NCOA2 (RC212235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCOA2. Mouse IgG1 OTI7G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NCOA2 antibody

Detection of Human SRC2 in Nuclear (A) or Cytosolic (S100) (B) extracts of Hela cells using AP05333PU-N SRC2 antibody at 20mcg/10mg lysate. Immunoprecipitate was resolved by SDS-PAGE and the identity of the SRC2 band verified by Mass Spectroscopy. Goat IgG Purified Hu, Ms IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

NCOA2 pSer736 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NCoA2 (Phospho-Ser736) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
50 µg / €230.00
  Assay Biotech

NCOA2 pSer736 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NCoA2 (Phospho-Ser736) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €330.00
  Assay Biotech

NCOA2 antibody

NCOA2 Rabbit Aff - Purified Hu E, P
50 µg / €230.00
  Assay Biotech

NCOA2 antibody

NCOA2 Rabbit Aff - Purified Hu E, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

NCOA2 - human gene knockout kit via CRISPR

NCOA2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NCOA2 - human gene knockout kit via CRISPR

NCOA2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NCOA2 - human gene knockout kit via CRISPR

NCOA2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

NCOA2 overexpression lysate

NCOA2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

NCOA2-knockout Cell lysate

NCOA2-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NCOA2 (mGFP-tagged) - Human nuclear receptor coactivator 2 (NCOA2), 200 uL, >10^7 TU/mL

Lenti ORF particles, NCOA2 (mGFP-tagged) - Human nuclear receptor coactivator 2 (NCOA2), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, NCOA2 (Myc-DDK tagged) - Human nuclear receptor coactivator 2 (NCOA2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, NCOA2 (Myc-DDK tagged) - Human nuclear receptor coactivator 2 (NCOA2) , 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

16 Item(s)

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