NCOA3 antibody

Principal name

NCOA3 antibody

Alternative names for NCOA3 antibody

NCoA-3, Nuclear receptor coactivator 3, SRC-3, Steroid receptor coactivator protein 3, TRAM-1, TRAM1, RAC-3, RAC3, AIB-1, AIB1, pCIP, ACTR, BHLHE42, Thyroid hormone receptor activator molecule 1, Receptor-associated coactivator 3, Amplified in breast cancer 1 protein, CBP-interacting protein, Class E basic helix-loop-helix protein 42

SwissProt ID

O09000 (Mouse), O57539 (Xenla), Q9EPU2 (Rat), Q9UPG7 (Human), Q9Y6Q9 (Human)

Gene ID

8202 (NCOA3)

Available reactivities

Hu (Human), Bov (Bovine), Macaque, Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine), Bt (Bat), Can (Canine), Eq (Equine), Hst (Hamster), Op (Opossum), Prim (Primate), Rb (Rabbit), Tr (Turkey), L (Lizard), Bear, GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of NCOA3 antibody

NCOA3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

General readings

Luo,J.H., (2008) Int. J. Cancer 122 (11), 2554-2561

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCOA3 antibody

  Rabbit polyclonal anti-NCOA3 antibody  
WB using Anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Rabbit IgG Aff - Purified Hu, Mky E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCOA3 antibody

  Rabbit Polyclonal Anti-NCOA3 Antibody  
WB Suggested Anti-NCOA3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCOA3 antibody

  Rabbit Polyclonal Anti-NCOA3 Antibody  
WB Suggested Anti-NCOA3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCOA3 antibody

  Rabbit Polyclonal Anti-NCOA3 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: (NCOA3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCOA3 (C-term) antibody

AM06052PU-N NCOA3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 10 mg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 AC3 Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCOA3 antibody

Western blot analysis using NCOA3 antibody Cat.-No AM06280SU-N against truncated Trx-NCOA3 recombinant protein (1) and truncated NCOA3 (aa1-200)-hIgGFc transfected CHOK1 cell lysate (2). Mouse IgG1 2C11B12 Ascites Hu WB
0.1 ml / €400.00
  Acris Antibodies GmbH

NCOA3 (188-198) antibody

Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr P
50 µg / €470.00
  Acris Antibodies GmbH

NCOA3 antibody

Detection of human SRC3 in nuclear extracts of HeLa cells by Western blotting with Rabbit anti Human SRC3 (SP2070P). Immunoprecipitation of the nuclear extracts was done using SP2070P followed by resolution by SDS-PAGE , staining with Coomassie and the SRC3 band confirmed by mass spectroscopy Rabbit Aff - Purified Hu IP
50 µg / €330.00
  Acris Antibodies GmbH

NCOA3 antibody

NCOA3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NCOA3 antibody

NCOA3 Mouse IgG1 Hu WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

NCOA3 antibody

Western blot using NCOA3 polyclonal antibody ( Cat # PAB10282 ) shows detection of NCOA3 in mouse liver nuclear extract ( lane 1 ), transient transfected 293 cell lysate ( lane 2 ), HeLa whole cell lysate ( lane 3 ) and mouse thyroid cell nuclear extract ( lane 4 ).
The band at ~ 148 kDa, indicated by the arrowhead, corresponds to NCOA3.
Mouse NCOA3 ( lanes 1 and 4 ) appear as ~ 105 kDa bands.
Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining ( not shown ).
Personal communication, H. Ying, NCI, Bethesda, MD. Bov, Hu, Macaque, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

NCOA3 (1351-1364) antibody

NCOA3 Chicken Liquid Hu, Rt E
0.2 mg / €570.00
  AgriSera AB

NCOA3 antibody

Human Tonsil; Immunohistochemistry with Human Tonsil lysate tissue at an antibody concentration of 5.0ug/ml using anti-NCOA3 antibody (ARP32487_P050); NCOA3 antibody - middle region (ARP32487_P050) in Human Tonsil cells using Immunohistochemistry Rabbit Aff - Purified Hu, Por P, WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

NCOA3 - human gene knockout kit via CRISPR

NCOA3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NCOA3 - human gene knockout kit via CRISPR

NCOA3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NCOA3 - human gene knockout kit via CRISPR

NCOA3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NCOA3 - human gene knockout kit via CRISPR

NCOA3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCOA3

NCOA3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NCOA3

NCOA3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

NCOA3

NCOA3 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

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