NCOA3 antibody

Principal name

NCOA3 antibody

Alternative names for NCOA3 antibody

NCoA-3, Nuclear receptor coactivator 3, SRC-3, Steroid receptor coactivator protein 3, TRAM-1, TRAM1, RAC-3, RAC3, AIB-1, AIB1, pCIP, ACTR, BHLHE42, Thyroid hormone receptor activator molecule 1, Receptor-associated coactivator 3, Amplified in breast cancer 1 protein, CBP-interacting protein, Class E basic helix-loop-helix protein 42

SwissProt ID

O09000 (Mouse), O57539 (Xenla), Q9EPU2 (Rat), Q9UPG7 (Human), Q9Y6Q9 (Human)

Gene ID

8202 (NCOA3)

Available reactivities

Hu (Human), Bov (Bovine), Macaque, Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), PLA/Proximity ligation assay (PLA), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of NCOA3 antibody

KAT13B / AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation. The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits KAT3A / KAT3B as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate.
SRC3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

General readings

General / background references:Anzick SL et al. AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Science 277:965-8 (1997). PubMed PMID: 9252329
Li H et al. RAC3, a steroid/nuclear receptor-associated coactivator that is related to SRC-1 and TIF2. Proc Natl Acad Sci U S A 94:8479-84 (1997). PubMed PMID: 9238002
Takeshita A et al. TRAM-1, A novel 160-kDa thyroid hormone receptor activator molecule, exhibits distinct properties from steroid receptor coactivator-1. J Biol Chem 272:27629-34 (1997). PubMed PMID: 9346901

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCOA3 antibody

WB using Anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Rabbit IgG Aff - Purified Hu, Mky E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCOA3 antibody

WB Suggested Anti-NCOA3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCOA3 antibody

WB Suggested Anti-NCOA3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCOA3 (C-term) antibody

AM06052PU-N NCOA3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 10 mg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 AC3 Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCOA3 antibody

Western blot analysis using NCOA3 antibody Cat.-No AM06280SU-N against truncated Trx-NCOA3 recombinant protein (1) and truncated NCOA3 (aa1-200)-hIgGFc transfected CHOK1 cell lysate (2). Mouse IgG1 2C11B12 Ascites Hu WB
0.1 ml / €400.00
  Acris Antibodies GmbH

NCOA3 antibody

WB using Anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Rabbit IgG Aff - Purified Hu, Mky E, WB
25 µl / €190.00
  Acris Antibodies GmbH

NCOA3 antibody

Immunohistochemistry: AP20153PU-N NCOA3 Antibody staining of Paraffin Embedded Human Placenta at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Acris Antibodies GmbH

NCOA3 (188-198) antibody

Spleen, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr P
50 µg / €470.00
  Acris Antibodies GmbH

NCOA3 antibody

Detection of human SRC3 in nuclear extracts of HeLa cells by Western blotting with Rabbit anti Human SRC3 (SP2070P). Immunoprecipitation of the nuclear extracts was done using SP2070P followed by resolution by SDS-PAGE , staining with Coomassie and the SRC3 band confirmed by mass spectroscopy Rabbit Aff - Purified Hu IP
50 µg / €330.00
  Acris Antibodies GmbH

NCOA3 antibody

NCOA3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NCOA3 antibody

NCOA3 Mouse IgG1 Hu WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

NCOA3 antibody

Western blot using NCOA3 polyclonal antibody ( Cat # PAB10282 ) shows detection of NCOA3 in mouse liver nuclear extract ( lane 1 ), transient transfected 293 cell lysate ( lane 2 ), HeLa whole cell lysate ( lane 3 ) and mouse thyroid cell nuclear extract ( lane 4 ).
The band at ~ 148 kDa, indicated by the arrowhead, corresponds to NCOA3.
Mouse NCOA3 ( lanes 1 and 4 ) appear as ~ 105 kDa bands.
Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining ( not shown ).
Personal communication, H. Ying, NCI, Bethesda, MD. Bov, Hu, Macaque, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

NCOA3 (1351-1364) antibody

NCOA3 Chicken Liquid Hu, Rt E
0.2 mg / €460.00
  AgriSera AB

NCOA3 antibody

Human Tonsil; Immunohistochemistry with Human Tonsil lysate tissue at an antibody concentration of 5.0ug/ml using anti-NCOA3 antibody (ARP32487_P050); NCOA3 antibody - middle region (ARP32487_P050) in Human Tonsil cells using Immunohistochemistry Rabbit Aff - Purified Hu, Por P, WB
50 µg / €350.00
  AVIVA Systems Biology

NCOA3 antibody

Western Blot: AIB1 Antibody [NB100-314] Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP. Rabbit Aff - Purified Hu IP, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NCOA3 antibody

Western Blot: AIB1 Antibody [NB100-315] Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 (Cat. No. A300-348A) used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NCOA3 antibody

NCOA3 Mouse IgG1 AC3 Purified Hu C, ICC/IF, IP, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

NCOA3 antibody

Western Blot: AIB 1 Antibody [NB300-567] - Figure 1 shows a Western blot of human AIB1 in T47D cell lysate using NB300-567. Rabbit Aff - Purified Hu WB
0.1 mg / €400.00
  Novus Biologicals Inc.

NCOA3 antibody

Immunohistochemistry: AIB1 Antibody [NBP1-33720] - Immunohistochemical analysis of AIB1 on paraffin-embedded H1299 xenograft, using NBP1-33720. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

NCOA3 antibody

Western Blot: AIB1 Antibody (2C11B12) [NBP1-47424] - Western blot analysis using anti-NCOA3 monoclonal antibody against truncated Trx-AIB1 recombinant protein(1) and truncated AIB1(aa1-200)-hIgGFc transfected CHOK1 cell lysate (2). Mouse IgG1 2C11B12 Purified Hu E, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

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