NCS1 antibody

Principal name

NCS1 antibody

Alternative names for NCS1 antibody

NCS-1, FREQ, FLUP, Neuronal calcium sensor 1

SwissProt ID

P62166 (Human), P62167 (Chick), P62168 (Rat), Q2V8Y7 (Bovin), Q8BNY6 (Mouse)

Gene ID

23413 (NCS1), 14299 (Ncs1), 65153

Available reactivities

Rt (Rat), Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Ze (Zebrafish), African clawed frog, Mam (Mammalia), Xen (Xenopus), Dros (Drosophila), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast)

Available hosts

Rabbit, Chicken, Sheep, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of NCS1 antibody

The neuronal calcium sensor (NCS) frequenin proteins bind calcium through EF-hand motifs, are predominantly expressed in neurons and are implicated in the regulation of neurotransmitter release at the neuromuscular junction. NCS1 may co-localize with the dendritic marker MAP-2 and is widely distributed in the brain, spinal cord and dorsal root ganglia. NCS1 has been shown to regulate neurosecretion in a calcium-dependent manner, potentiates nitric oxide synthase activity, and can activate calcineurin and 3'-5' nucleotide phosphodiesterase. Frequenin is the term used to describe the invertebrate homolog of Neuronal Calcium Sensor-1 (NCS-1). The term NCS-1 has been reserved for the protein observed in human and mammals, whereas frequenin has been used to describe the homolog in Drosophila and Xenopus.

General readings

Nakamura T.Y., et al (2001). Proc. Natl. Acad. Sci. U.S.A. 98:12808-12813Bahi N., et al (2003). Hum. Mol. Genet. 12:1415-1425

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCS1 antibody

Sample(30 ug of whole cell lysate). A:A431. B:Hep G2. 12% SDS PAGE. TA309002 diluted at 1:200 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCS1 antibody

Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Grosse et al., (2000) J. Neurosci. 20: 1869-1882).  The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippo-campal cell cultures after 19 d in vitro.  Numer-ous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity.   NCS-1 derived from invertebrate homologs has been referred to as frequenin. Rabbit IgG Aff - Purified Hu, Mam, Ms, Rt C, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NCS1 antibody

WB Suggested Anti-FREQ Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Hu, Ms, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCS1 antibody

WB Suggested Anti-FREQ Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCS1 antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCS1 antibody

Formalin-Fixed and Paraffin-Embedded Human breast carcinoma reacted with NCS1 Antibody (Center) Cat.-No AP11588PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Bov, Chk, Hu, Ms, Rt, Xen F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NCS1 (C-term) antibody

NCS1 Sheep Purified Rt WB
1 ml / €340.00
  Acris Antibodies GmbH

NCS1 antibody

NCS1 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

NCS1 antibody

Western Blot analysis of FREQ expression in transfected 293T cell line (H00023413-T01) by FREQ MaxPab polyclonal antibody.

Lane 1: FREQ transfected lysate(20.9 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

NCS1 antibody

NCS1 Mouse IgG2a 3E7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NCS1 antibody

NCS1 Rabbit Hu E
0.4 ml / €390.00
  Abnova Taiwan Corp.

NCS1 antibody

Western blot analysis of FREQ with rat kidney tissue lysate. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NCS1 antibody

Hippocampal neurons were prepared from 17 days old NMRI mice and grown as described previously ( G. Grosse et al., ( 2000 ) J. Neurosci. 20 : 1869-1882 ) .
The figure shows the distribution of Freq ( red ) and synaptobrevin ( green ) in hippo-campal cell cultures after 19 days in vitro.
Numer- ous synapses immunoreactive for synaptobrevin also show Freq immunoreactivity. Rabbit Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

NCS1 antibody

Human Brain; FREQ antibody - N-terminal region (ARP34315_P050) in Human Brain cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

NCS1 antibody

Human Thymus; WB Suggested Anti-FREQ Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Thymus; FREQ antibody - middle region (ARP34711_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NCS1 antibody

Immunofluorescence: Frequenin Antibody [NB100-1686] - Figure. Hippocampal neurons were prepared  from 17 d old NMRI mice. The figure shows the  distribution of neuronal calcium sensor-1 (NCS-  1) (red) and synaptobrevin (green) in hippocampal  cell cultures after 19 d in vitro. Numerous  synapses immunoreactive for synaptobrevin  also show NCS-1 immunoreactivity. NCS-1  derived from invertebrate homologs has been  referred to as frequenin. Chicken Aff - Purified Hu, Mam, Ms, Rt E, ICC/IF, WB
0.2 mg / €510.00
  Novus Biologicals Inc.

NCS1 antibody

Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCS1 (transcript variant 1)

NCS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NCS1 (1-190, His-tag)

NCS1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

NCS1 (1-190, His-tag)

NCS1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

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