NCS1 antibody

Principal name

NCS1 antibody

Alternative names for NCS1 antibody

NCS-1, FREQ, FLUP, Neuronal calcium sensor 1

SwissProt ID

P62166 (Human), P62167 (Chick), P62168 (Rat), Q2V8Y7 (Bovin), Q8BNY6 (Mouse)

Gene ID

23413 (NCS1), 14299 (Ncs1), 65153

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Dros (Drosophila), Mam (Mammalia), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast)

Available hosts

Rabbit, Sheep, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of NCS1 antibody

Kit Component:
- KN202389G1, NCS1 gRNA vector 1 in pCas-Guide vector
- KN202389G2, NCS1 gRNA vector 2 in pCas-Guide vector
- KN202389D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Nakamura T.Y., et al (2001). Proc. Natl. Acad. Sci. U.S.A. 98:12808-12813Bahi N., et al (2003). Hum. Mol. Genet. 12:1415-1425

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NCS1 antibody

  Rabbit Polyclonal antibody to NCS1 (neuronal calcium sensor 1)  
Sample(30 ug of whole cell lysate). A:A431. B:Hep G2. 12% SDS PAGE. TA309002 diluted at 1:200 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NCS1 antibody

  Rabbit polyclonal Frequenin antibody  
Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Grosse et al., (2000) J. Neurosci. 20: 1869-1882).  The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippo-campal cell cultures after 19 d in vitro.  Numer-ous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity.   NCS-1 derived from invertebrate homologs has been referred to as frequenin. Rabbit IgG Aff - Purified Hu, Mam, Ms, Rt C, E, ICC/IF, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

NCS1 antibody

  Rabbit Polyclonal Anti-NCS1 Antibody  
WB Suggested Anti-FREQ Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Hu, Ms, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCS1 antibody

  Rabbit Polyclonal Anti-FREQ Antibody  
WB Suggested Anti-FREQ Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NCS1 antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NCS1 antibody

Formalin-Fixed and Paraffin-Embedded Human breast carcinoma reacted with NCS1 Antibody (Center) Cat.-No AP11588PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Bov, Chk, Hu, Ms, Rt, Xen F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NCS1 (C-term) antibody

NCS1 Sheep Purified Rt WB
1 ml / €340.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

NCS1 - human gene knockout kit via CRISPR

NCS1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NCS1 - human gene knockout kit via CRISPR

NCS1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NCS1 - human gene knockout kit via CRISPR

NCS1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NCS1 - human gene knockout kit via CRISPR

NCS1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NCS1 (transcript variant 1)

  Recombinant protein of human frequenin homolog (Drosophila) (FREQ), transcript variant 1  
NCS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NCS1 (1-190, His-tag)

NCS1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

NCS1 (1-190, His-tag)

NCS1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

NCS1 (1-190, His-tag)

NCS1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

NCS1 (1-190, His-tag)

NCS1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

NCS1 overexpression lysate

NCS1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NCS1 (mGFP-tagged) - Human neuronal calcium sensor 1 (NCS1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, NCS1 (mGFP-tagged) - Human neuronal calcium sensor 1 (NCS1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, NCS1 (mGFP-tagged) - Human neuronal calcium sensor 1 (NCS1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, NCS1 (mGFP-tagged) - Human neuronal calcium sensor 1 (NCS1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, NCS1 (Myc-DDK tagged) - Human neuronal calcium sensor 1 (NCS1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, NCS1 (Myc-DDK tagged) - Human neuronal calcium sensor 1 (NCS1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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