NDC80 antibody

Principal name

NDC80 antibody

Alternative names for NDC80 antibody

HEC, HEC1, KNTC2

SwissProt ID

O14777 (Human)

Gene ID

10403 (NDC80), 1040374 (yhcQ)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ce (C. elegans), Bov (Bovine), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), EPair for Elisa (EPair), ELISA (detection) (E(detection))

Background of NDC80 antibody

HEF1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.

General readings

Chen Y, Riley DJ, Chen PL, Lee WH.
HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis.
Mol Cell Biol. 1997 Oct;17(10):6049-56.
PMID: 9315664

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NDC80 (C-term) antibody

TA302826 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NDC80 antibody

Western blot analysis of extracts from Jurkat cells, using KNTC2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NDC80 antibody

Western blot analysis of extracts of various cell lines, using NDC80 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NDC80 antibody

Western blot analysis on Jurkat,A549 and HuvEc cell lysate using HEC1 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NDC80 antibody

ELISA: NDC80 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

NDC80 (630-642) antibody

Human Adrenal (formalin-fixed, paraffin-embedded) stained with NDC80 antibody AP22482PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Hu, Mky, Prim E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NDC80 antibody

NDC80 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

NDC80 antibody

NDC80 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

NDC80 antibody

Western Blot analysis of NDC80 expression in transfected 293T cell line (H00010403-T02) by NDC80 MaxPab polyclonal antibody.

Lane 1: NDC80 transfected lysate(73.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NDC80 antibody

KNTC2 monoclonal antibody (M01), clone 1A10 Western Blot analysis of KNTC2 expression in HeLa NE ( Cat # L013V3 ). Mouse IgG1 1A10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NDC80 antibody

NDC80 polyclonal antibody ( Cat # PAB6236 ) staining ( 0.5 µg/ml ) of HeLa lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NDC80 antibody

Immunofluorescence analysis of HuvEc cells, using KNTC2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

NDC80 antibody

Immunofluorescence analysis of HuvEc cells, using KNTC2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

NDC80 antibody

Western blot analysis of NDC80 expression in HEK293T (A); SP2/0 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NDC80 antibody

Western blot analysis of NDC80 expression in HEK293T (A); SP2/0 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NDC80 antibody

Western blot analysis of NDC80 expression in HEK293T (A); SP2/0 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NDC80

NDC80 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NDC80

NDC80 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NDC80

NDC80 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

NDC80

NDC80 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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