NDEL antibody

Principal name

NDEL antibody

Alternative names for NDEL antibody

EOPA, MITAP1, NUDEL, Nuclear distribution protein nudE-like 1

SwissProt ID

Q9GZM8 (Human)

Gene ID

81565 (NDEL1)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Mky (Monkey), Rt (Rat)

Available hosts

Rabbit, Goat, Mouse

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of NDEL antibody

Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50% identity with mouse and human NUDE proteins.

General readings

Yingling J, Youn YH, Darling D, Toyo-Oka K, Pramparo T, Hirotsune S, Wynshaw-Boris A.
Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division.
Cell 2008 Feb 132 (3): 474-86.
PMID: 18267077

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NDEL antibody

  NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)  
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NDEL1 monoclonal antibody. Mouse IgG2b OTI1G10 Purified Can, Hu, Mky, Rt F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NDEL antibody

  NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI1C7 (formerly 1C7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503061) Mouse IgG2b OTI1C7 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NDEL antibody

  NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI3B9 (formerly 3B9)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503204) Mouse IgG2a OTI3B9 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NDEL antibody

  NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI4E5 (formerly 4E5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503352) Mouse IgG2a OTI4E5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NDEL antibody

  NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI5E11 (formerly 5E11)  
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503353) Mouse IgG2a OTI5E11 Purified Hu, Ms F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NDEL (both isoforms) antibody

  Goat Anti-NDEL1 Antibody  
TA305793 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NDEL antibody

  Rabbit Polyclonal Nudel Antibody  
Western blot analysis of Nudel in Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NDEL antibody

  Rabbit anti-NDEL1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using NDEL1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NDEL antibody

  Rabbit Polyclonal Anti-NDEL1 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (NDEL1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

NDEL antibody

  NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1. Mouse IgG2a OTI1G7 Purified Can, Hu, Ms, Rt WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NDEL antibody

  NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI5C2 (formerly 5C2)  
Anti-NDEL1 mouse monoclonal antibody (TA503313) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDEL1(RC212323). Mouse IgG2a OTI5C2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NDEL antibody

Immunocytochemical detection of 050603-1 Phospho-NUDEL on Methanol fixed HeLa cells with AM26510AF-N. Mouse IgG1 N219TP Azide Free Hu, Ms ICC/IF, P, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

NDEL antibody

Western Blot analysis of NDEL1 expression in transfected 293T cell line (H00081565-T01) by NDEL1 MaxPab polyclonal antibody.

Lane 1: NDEL1 transfected lysate(38.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NDEL antibody

NDEL1 polyclonal antibody ( Cat # PAB11614 ) ( 0.1 µg/ml ) staining of human cerebellum lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NDEL antibody

Immunocytochemistry of NDEL1 in Jurkat cells with NDEL1 polyclonal antibody ( Cat # PAB12877 ) at 2 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

NDEL1 - human gene knockout kit via CRISPR

NDEL1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NDEL1 - human gene knockout kit via CRISPR

NDEL1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NDEL1 - human gene knockout kit via CRISPR

NDEL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NDEL1 - human gene knockout kit via CRISPR

NDEL1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NDEL (transcript variant 2)

  Recombinant protein of human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 2  
NDEL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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