NEC2 antibody

Principal name

NEC2 antibody

Alternative names for NEC2 antibody

PCSK2, Neuroendocrine convertase 2,Prohormone convertase 2, Proprotein convertase 2, KEX2-like endoprotease 2

SwissProt ID

P16519 (Human), P21661 (Mouse), P28841 (Rat), Q03333 (Pig), Q5REC2 (Ponab), Q9GLR0 (Bovin)

Gene ID

5126 (PCSK2), 18549 (Pcsk2), 100171744

Available reactivities

Ms (Mouse), Hu (Human), Mky (Monkey), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of NEC2 antibody

PC2 was initially found as an insulin processing enzyme, and was the third of the human prohormone convertasae enzymes to be discovered. Like furin, PC1 and PACE4, PC2 is a serine proteinase that cleaves after pairs of basic amino acids, but with some differences in substrate specificity and distribution. The catalytic domain has homology to other PC enzymes, the prohormone convertase family initially discovered for their role in processing POMC, insulin and other pro-hormones, and later found to process a wider range of precursor proteins. Some have renamed the prohormone convertases as proprotein convertases, and another group has renamed the entire family with a uniform nomenclature of SPCs ( s ubtilisin-like p roprotein c onvertases), with PC2 getting the SPC2 moniker. PC2 structure contains a propeptide domain, a catalytic domain and an RGD containing cystein-rich domain (homo-B). The PCs have an RxxR consensus cleavage requirement, and the propeptide is separated from the mature protein by just such a sequence. After cleavage of the propeptide domain, PC2 becomes a two-chain form, and the propeptide piece is released after a second internal cleavage. PC2 resides along with PC1, mainly in the dense core secretory vesicles and the TGN, and is cycled to the surface, with some of the PC2 being secreted from the cells. PC2 has been reported to be elevated in tumor cell lines and to increase tumor progression. PC2 is expressed at low levels in most tissues, but found in highest concentration in the brain, the anterior pituitary, pancreatic islet cells, thyroid, parathyroid and gut. PC2 cleaves a wide variety of proteins, including growth factors, viral coat proteins, matrix metalloproteinases, mostly after PC1 cleavage. The maturation of PC2 appears to require association with the 7B2 protein, which both helps fold PC2 properly and keeps the PC2 latent until the 7B2 protein is clipped by a PC enzyme.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEC2 (N-term) antibody

Western blot analysis of PCSK2 Antibody (N-term) (Cat. #TA324950) in mouse cerebellum tissue lysates (35ug/lane). PCSK2 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NEC2 antibody

WB Suggested Anti-PCSK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NEC2 antibody

NEC2 Rabbit Serum Hu, Mky, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

NEC2 antibody

PCSK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PCSK2 expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NEC2 antibody

NEC2 Mouse IgG2a 3H4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEC2 antibody

NEC2 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

NEC2 antibody

NEC2 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

NEC2 antibody

Western Blot: Proprotein Convertase 2 Antibody  [NBP1-32186]-(30 µg of whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:500 Rabbit IgG Hu WB
0.1 ml / €420.00
  Novus Biologicals Inc.

NEC2 antibody

NEC2 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEC2

NEC2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NEC2

NEC2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NEC2

NEC2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

NEC2

NEC2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NEC2

NEC2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

PCSK2 overexpression lysate

PCSK2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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