Necdin antibody

Principal name

Necdin antibody

Alternative names for Necdin antibody

NDN

SwissProt ID

P25233 (Mouse), Q99608 (Human)

Gene ID

4692 (NDN)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), EPair for Elisa (EPair), ELISA (detection) (E(detection))

Background of Necdin antibody

Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.

General readings

Chapman, E.J., et al. Mol. Carcinog. 48(11):975-981(2009)
Yoshikawa, K. No To Hattatsu 41(3):214-218(2009)
Liu, X., et al. Mol. Cell. Neurosci. 41(1):51-61(2009)
de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Lau, J.C., et al. Nucleic Acids Res. 32(11):3376-3382(2004)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Necdin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506975) Mouse IgG2a OTI5D1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Necdin antibody

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1B3 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Necdin antibody

Immunoperoxidase of monoclonal antibody to NDN on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml] Mouse IgG2a 1B3 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Necdin antibody

WB Suggested Anti-NDN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Necdin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDN (RC200448, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDN. Mouse IgG1 OTI3A12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Necdin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDN (RC200448, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDN. Mouse IgG1 OTI1B1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Necdin (N-term) antibody

Western blot analysis of Necdin Antibody (N-term) Cat.-No AP52832PU-N in Ramos cell line lysates (35ug/lane). This demonstrates the NDN antibody detected the NDN protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Necdin antibody

Western Blot analysis of NDN expression in transfected 293T cell line (H00004692-T01) by NDN MaxPab polyclonal antibody.

Lane 1: NDN transfected lysate(35.31 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Necdin antibody

Western Blot analysis of NDN expression in transfected 293T cell line (H00004692-T01) by NDN MaxPab polyclonal antibody.

Lane 1: NDN transfected lysate(36.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Necdin antibody

Necdin Mouse IgG2a 3B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Necdin antibody

Necdin Mouse IgG2a 3C1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Necdin antibody

NDN monoclonal antibody (M07), clone 2D11 Western Blot analysis of NDN expression in U-2 OS ( Cat # L022V1 ). Mouse IgG2a 2D11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Necdin antibody

Necdin Mouse IgG2b 2G8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Necdin antibody

Human Brain; WB Suggested Anti-NDN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; NDN antibody - middle region (ARP56358_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Necdin antibody

Western Blot: Necdin Antibody [NBP1-58244] - This Anti-NDN antibody was used in Western Blot of Fetal Brain   tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Necdin

Necdin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Necdin

Necdin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Necdin

Necdin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

NDN (Human) IP-WB Antibody Pair

NDN (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

NDN (Human) IP-WB Antibody Pair

NDN (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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