NEDD4 antibody

Principal name

NEDD4 antibody

Alternative names for NEDD4 antibody

KIAA0093, E3 ubiquitin-protein ligase NEDD4

SwissProt ID

P46934 (Human), P46935 (Mouse), Q62940 (Rat)

Gene ID

4734 (NEDD4), 17999 (Nedd4), 25489

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chimp (Chimpanzee), Macaque, Rb (Rabbit), Can (Canine), Eq (Equine), Chk (Chicken), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NEDD4 antibody

Nedd4-1 is an E3 ubiquitin ligase that contains a C2 domain, three or four WW domains, and a ubiquitin ligase Hect domain, and it belongs to the Nedd4 family of Hect E3 ligases that also includes its relative Nedd4-2 (1, 2). The WW domain of Nedd4 proteins recognizes and binds a short amino acid sequence in substrate proteins, called the PY motif (L/PPxY) (3, 4). PTEN is a tumor suppressor protein that frequently exhibited mutations and it is suggested that PTEN stability and sub-cellular distribution is mediated by E3 ubiquitin ligase Nedd4. In most of the cancers the PTEN, a critical regulator of multiple cellular processes is either mutated or deleted and these changes have profound impacts on carcinogenesis. The Nedd4 negatively regulated PTEN stability by catalyzing PTEN ubiquitination via Nedd4. The ubiquitin ligase Nedd4 has been proposed to regulate a number of signaling pathways, but its physiological role in mammals has not been characterized. Nedd4 knockout mouse exhibited reduced insulin-like growth factor 1 (IGF1) and insulin signaling, delayed embryonic development, reduced body weight neonatal lethality, embryonic fibroblasts mitotic activity is reduced and the Nedd4 adapter protein grb10 is increased (5).
Nedd4 has at least 2 homologues, Nedd4-1 and Nedd4-2, the human genomic loci of these two homologues are different (Nedd4-1 is on 15q21 and 18q21 for Nedd4-2). Only the Nedd4-1 was found associated with ubiquinated PTEN while Nedd4-2 has no ubiquitin ligase activity (6). Nedd4-1 is 887 amino acids long with approximately 115kDa protein abundantly found in HeLa S100 cells. Mouse Nedd4-2 is slightly larger in size with 1004 amino acids.

General readings

Lin, Q., et al. Mol. Cell. Biol. 30(6):1541-1554(2010)
Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
Edwin, F., et al. J. Biol. Chem. 285(1):255-264(2010)
Guo, Y.Y., et al. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 31(6):679-685(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEDD4 antibody

Western blot using affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates.  The blot was blocked with B501-0500 5% BLOTTO overnight at 4°C.  Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blotting.  ECL was used for detection. Rabbit IgG Aff - Purified Can, Chimp, Eq, Hu, Macaque, Rb E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEDD4 (395-462) antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

NEDD4 antibody

Western blot using affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates.  The blot was blocked with B501-0500 5% BLOTTO overnight at 4°C.  Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blotting.  ECL was used for detection. Rabbit IgG Aff - Purified Can, Chimp, Eq, Hu, Macaque, Rb E, WB
25 µl / €190.00
  Acris Antibodies GmbH

NEDD4 Control Peptide

NEDD4
0.25 mg / €250.00
  Acris Antibodies GmbH

NEDD4 (847-868) antibody

NEDD4 Rabbit Aff - Purified Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

NEDD4 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue reacted with NEDD4 Antibody (C-term) Cat.-No AP52847PU-N, which was peroxidase conjugated to the secondary antibody and followed by  DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for Clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NEDD4 antibody

NEDD4 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

NEDD4 antibody

Western blot using NEDD4 polyclonal antibody ( Cat # PAB10283 ) shows detection of a 115 kDa band corresponding to endogenous NEDD4 ( arrowhead ) in MDA-MB-435 cell lysates.
The blot was blocked with 5% BLOTTO overnight at 4°C.
Primary antibody was used at a 1 : 350 dilution in 5% BLOTTO followed by reaction with a 1 : 20,000 dilution of HRP goat anti-rabbit IgG.
ECL was used for detection. Bov, Chimp, Hu, Macaque, Rb
0.1 mg / €560.00
  Abnova Taiwan Corp.

NEDD4 antibody

Mouse adipose; . Lanes: Lane 1: 50ug Mouse adipose lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Donkey anti-Rabbit-HRP. Secondary Antibody Dilution: 1:10000. Gene Name: NEDD4. Submitted by: Libin Liu, Boston University. .; NEDD4 antibody - middle region (ARP43067_P050) in Mouse adipose cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NEDD4 antibody

NEDD4 Rabbit Serum Hu, Ms, Rt P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEDD4 (transcript variant 1)

NEDD4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NEDD4

NEDD4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NEDD4

NEDD4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NEDD4 overexpression lysate

NEDD4 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

NEDD4 overexpression lysate

NEDD4 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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