NEDD8 antibody

Principal name

NEDD8 antibody

Alternative names for NEDD8 antibody

Neural precursor cell expressed developmentally down-regulated protein 8

SwissProt ID

P29595 (Mouse), Q15843 (Human), Q71UE8 (Rat)

Gene ID

4738 (NEDD8), 18002 (Nedd8), 25490

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NEDD8 antibody

Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family. The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process alogous to ubiquitition. NEDD8 is first synthesized in a precursor form with a carboxy-termil extension peptide that is removed by either the UCH-L3 or NEDP1/DEN1 hydrolase protein to yield a mature NEDD8 protein. Mature NEDD8 is then covalently linked to target proteins via the carboxy-termil glycine residue in a reaction catalyzed by the APP-BP1/Uba3 heterodimer complex and Ubc12 as the E1- and E2-like enzymes, respectively. An E3 ligase protein, Roc1/Rbx1, is also required for neddylation of the cullin proteins. Protein de-neddylation is catalyzed by a number of enzymes in the cell, including a "ubiquitin-specific" protease USP21, the NEDP1/DEN1 hydrolase and the COP9/siglosome (CSN). In contrast to the ubiquitin pathway, the NEDD8 modification system acts on only a few substrates and does not appear to target proteins for degradation. Neddylation of cullin proteins activates the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex by promoting complex formation and enhancing the recruitment of the E2-ubiquitin intermediate. While NEDD8 modification of VHL is not required for ubiquitition of HIF1-a, it is required for fibronectin matrix assembly. Mdm2-dependent neddylation of p53 inhibits its transcriptiol activity

General readings

Wu K., et al. (2002) J Biol Chem. 277(1): 516-27.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEDD8 antibody

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NEDD8 mouse mAb with DAB staining. Mouse IgG1 1A7 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NEDD8 antibody

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using NEDD8 antibody Cat.-No AM06719PU-N with DAB staining. Mouse IgG1 5B8 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

NEDD8 antibody

  Rabbit polyclonal anti-NEDD8 antibody  
Western blot analysis of extracts from Jurkat cells, using NEDD8 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NEDD8 antibody

  NEDD8 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using NEDD8 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEDD8 antibody

  Rabbit Polyclonal Anti-NEDD8 Antibody  
Western blot analysis on Jurkat cell lysate using NEDD8 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEDD8 antibody

Immunohistochemistry (IHC) analyzes of NEDD8 antibody (Cat.-No.: AP06450PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

NEDD8 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NEDD8 (N-term) antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NEDD8 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

NEDD8 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using NEDD8 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

NEDD8 - human gene knockout kit via CRISPR

NEDD8 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NEDD8 - human gene knockout kit via CRISPR

NEDD8 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NEDD8 - human gene knockout kit via CRISPR

NEDD8 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NEDD8 - human gene knockout kit via CRISPR

NEDD8 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nedd8 - mouse gene knockout kit via CRISPR

Nedd8 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nedd8 - mouse gene knockout kit via CRISPR

Nedd8 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nedd8 - mouse gene knockout kit via CRISPR

Nedd8 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEDD8 (N-term HIS tag)

  Purified recombinant protein of Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8), with N-terminal HIS tag, expressed in E.Coli, 50ug  
NEDD8 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

NEDD8 (1-76, His-tag)

NEDD8 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

NEDD8 (1-76, His-tag)

NEDD8 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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