NEDD9 antibody

Principal name

NEDD9 antibody

Alternative names for NEDD9 antibody

CASL, CASS2, hEF1, Enhancer of filamentation 1,Neural precursor cell expressed developmentally down-regulated protein 9

SwissProt ID

Q14511 (Human)

Gene ID

4739 (NEDD9)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of NEDD9 antibody

HEF1, also known as Enhancer of filamentation 1, CRKassociated substrate-related protein, CAS-L, CasL, p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene. HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1. This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts, it is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells, it is localized predominantly in the cell periphery with particular concentration in lamellipodia, but it is also found in the nucleus. HEF1 is widely expressed although higher levels are detected in kidney, lung, and placental tissue. HEF1 is also detected in T-cells, B-cells and diverse cell lines. HEF1 is activated upon induction of cell growth. Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle.

General readings

Kim M, Gans JD, Nogueira C, Wang A, Paik JH, Feng B, Brennan C, Hahn WC, Cordon-Cardo C, Wagner SN, Flotte TJ, Duncan LM, Granter SR, Chin L.
Comparative oncogenomics identifies NEDD9 as a melanoma metastasis gene.
Cell. 2006 Jun 30;125(7):1269-81.
PMID: 16814714

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEDD9 antibody

TA303260 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEDD9 antibody

WB using monoclonal anti-HEF1 antibody antibody shows detection of a 115 kDa band corresponding to HEF1 in MCF7 lysate (arrowhead). Approximately 35 ug of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye®800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). Mouse 2G9 Purified Hu E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEDD9 antibody

Western blotting using Monoclonal anti-HEF1 antibody (clone 14A11) shows detection of HEF1 present in MCF-7 cells induced to express HEF1 by tetracycline removal (right lane). See Pugacheva et al for details. Mouse 14A11 Purified Hu E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEDD9 antibody

WB Suggested Anti-NEDD9 Antibody Titration: 5.0 ug/ml; Positive Control: 721_B cell lysate NEDD9 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEDD9 antibody

WB Suggested Anti-NEDD9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NEDD9 pSer369 antibody

NEDD9 Rabbit Serum Hu E, ICC/IF, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

NEDD9 antibody

NEDD9 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NEDD9 antibody

Immunofluorescence of purified MaxPab antibody to NEDD9 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

NEDD9 antibody

NEDD9 Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

NEDD9 antibody

NEDD9 MaxPab rabbit polyclonal antibody. Western Blot analysis of NEDD9 expression in mouse brain. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NEDD9 antibody

NEDD9 Mouse IgG1 1B4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEDD9 antibody

NEDD9 Mouse IgG1 1B4 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

NEDD9 antibody

Immunofluorescence microscopyusing NEDD9 monoclonal antibody, clone 14A11 ( Cat # MAB2091 ) shows detectionof NEDD9 localized at the centrosome ( bright dots ) and focal adhesion sites.
The antibody was used at a 1:100 dilution.
Personal Communication. Elena Pugacheva, FoxChase Cancer Center, Philadelphia, PA. Mouse IgG2b 14A11 Purified Hu E, ICC/IF, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

NEDD9 antibody

NEDD9 Mouse IgG1 2G9 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

NEDD9 antibody

NEDD9 polyclonal antibody ( Cat # PAB7323 ) ( 0.1 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NEDD9 antibody

Human kidney; NEDD9 antibody - middle region (ARP51210_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

NEDD9 antibody

Human 293T; WB Suggested Anti-NEDD9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 293T cell lysate; NEDD9 antibody - middle region (ARP51211_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NEDD9 antibody

HEF-1 Antibody [2G9] Mouse IgG1 2G9 Purified Hu, Ms, Rt C, F, ICC/IF, IP, WB
50 µl / €360.00
  Immuquest

NEDD9 antibody

HEF1 Antibody [2G9] Mouse IgG1 2G9 Purified Hu, Ms, Rt C, F, ICC/IF, IP, WB
0.1 ml / €400.00
  Immuquest

NEDD9 antibody

NEDD9 Mouse IgG2b 14A11 Purified Hu ICC/IF, IP
50 µl / €360.00
  Immuquest

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