NEDL2 antibody

Principal name

NEDL2 antibody

Alternative names for NEDL2 antibody

HECT, HECW2, KIAA1301, NEDD4-like E3 ubiquitin-protein ligase 2

SwissProt ID

Q9P2P5 (Human)

Gene ID

57520 (HECW2)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Fe (Feline)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NEDL2 antibody

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Miyazaki, K., et al. Biochem. Biophys. Res. Commun. 308(1):106-113(2003)

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEDL2 (Center) antibody

Western blot analysis of HECW2 Antibody (Center) Cat.-No AP52027PU-N in mouse brain tissue lysates (35ug/lane). This demonstrates the HECW2 antibody detected the HECW2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NEDL2 antibody

NEDL2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NEDL2 antibody

Detection limit for recombinant GST tagged HECW2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2b 5H1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEDL2 antibody

NEDL2 Mouse IgG2b 1A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEDL2 antibody

NEDL2 Goat Hu, Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

NEDL2 antibody

Western blot analysis of extracts from LOVO/HepG2 cells, using HECW2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

NEDL2 antibody

Western blot analysis of extracts from LOVO/HepG2 cells, using HECW2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

NEDL2 antibody

Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NEDL2 antibody

Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NEDL2 antibody

Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

NEDL2 antibody

NEDL2 Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

NEDL2 antibody

NEDL2 Rabbit IgG Aff - Purified Hu E, WB
50 µg / €290.00
  Novus Biologicals Inc.

NEDL2 antibody

Immunohistochemistry-Paraffin: NEDL2 Antibody [NBP1-92174] - Immunohistochemical staining of human lung shows strong cytoplasmic positivity in subsets of alveolar cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEDL2

NEDL2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NEDL2

NEDL2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NEDL2

NEDL2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

HECW2 overexpression lysate

HECW2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

17 Item(s)

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