NEDL2 antibody

Principal name

NEDL2 antibody

Alternative names for NEDL2 antibody

HECT, HECW2, KIAA1301, NEDD4-like E3 ubiquitin-protein ligase 2

SwissProt ID

Q9P2P5 (Human)

Gene ID

57520 (HECW2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NEDL2 antibody

Kit Component:
- KN216544G1, HECW2 gRNA vector 1 in pCas-Guide vector
- KN216544G2, HECW2 gRNA vector 2 in pCas-Guide vector
- KN216544D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Miyazaki, K., et al. Biochem. Biophys. Res. Commun. 308(1):106-113(2003)

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEDL2 (Center) antibody

Western blot analysis of HECW2 Antibody (Center) Cat.-No AP52027PU-N in mouse brain tissue lysates (35ug/lane). This demonstrates the HECW2 antibody detected the HECW2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NEDL2 antibody

NEDL2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NEDL2 antibody

Detection limit for recombinant GST tagged HECW2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2b 5H1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEDL2 antibody

NEDL2 Mouse IgG2b 1A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEDL2 antibody

NEDL2 Goat Hu, Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

NEDL2 antibody

Western blot analysis of extracts from LOVO/HepG2 cells, using HECW2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

NEDL2 antibody

Western blot analysis of extracts from LOVO/HepG2 cells, using HECW2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

NEDL2 antibody

Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NEDL2 antibody

Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NEDL2 antibody

Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

HECW2 - human gene knockout kit via CRISPR

HECW2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

HECW2 - human gene knockout kit via CRISPR

HECW2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

HECW2 - human gene knockout kit via CRISPR

HECW2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HECW2 - human gene knockout kit via CRISPR

HECW2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEDL2

NEDL2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

NEDL2

NEDL2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NEDL2

NEDL2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

HECW2 overexpression lysate

HECW2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, HECW2 (mGFP-tagged) - Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), 200 uL, >10^7 TU/mL

Lenti ORF particles, HECW2 (mGFP-tagged) - Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, HECW2 (Myc-DDK tagged) - Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, HECW2 (Myc-DDK tagged) - Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2) , 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

20 Item(s)

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