NEK2 antibody

Principal name

NEK2 antibody

Alternative names for NEK2 antibody

NLK1, NimA-related protein kinase 2, HSPK 21, Serine/threonine-protein kinase Nek2

SwissProt ID

O35942 (Mouse), P51955 (Human)

Gene ID

4751 (NEK2)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of NEK2 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEK2 antibody

Western blot analysis of extracts of various cell lines, using NEK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEK2 antibody

Immunofluorescence of monoclonal antibody to NEK2 on HeLa cell. [antibody concentration 25 ug/ml] Mouse IgG2a 3B7 Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEK2 antibody

Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein.  The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.  Personal communication Steven Pelech, Kinexus Inc. Rabbit Aff - Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEK2 antibody

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed monoclonal antibody against NEK-2 (clone 8A6). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 8A6 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEK2 (331-445) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2F6 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

NEK2 antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NEK2 antibody

The anti-NEK2 Pab is used in Western blot to detect NEK2 in HeLa cell lysate. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NEK2 antibody

Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NEK2 antibody

Immunofluorescence of purified MaxPab antibody to NEK2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu, Rt E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

NEK2 antibody

Immunofluorescence of purified MaxPab antibody to NEK2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu, Rt E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

NEK2 antibody

NEK2 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

NEK2 antibody

Western Blot analysis of NEK2 expression in transfected 293T cell line (H00004751-T02) by NEK2 MaxPab polyclonal antibody.

Lane 1: NEK2 transfected lysate(51.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NEK2 antibody

Immunoperoxidase of monoclonal antibody to NEK2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml] Mouse IgG2a 2F6 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEK2 antibody

Immunofluorescence of monoclonal antibody to NEK2 on HeLa cell. [antibody concentration 30 ug/ml] Mouse IgG2a 2F9 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEK2 antibody

Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 2A10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEK2 antibody

Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 1C8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEK2 antibody

The NEK2 polyclonal antibody ( Cat # PAB3283 ) is used in Western blot to detect NEK2 in HeLa cell lysate. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NEK2 antibody

Western blot analysis of NEK2 polyclonal antibody ( Cat # PAB3284 ) in mouse lung tissue lysate. NEK2 ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NEK2 antibody

Western blot analysis is shown using NEK2 polyclonal antibody ( Cat # PAB10072 ) to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate ( arrowhead ). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature followed by detection using standard techniques.
Personal communication Steven Pelech, Kinexus Inc. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

NEK2 antibody

Western blot showing NEK2 polyclonal antibody ( Cat # PAB12347 ) on recombinant His6 protein. Sheep Hu WB
0.2 ml / €560.00
  Abnova Taiwan Corp.

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