NEK2 antibody

Principal name

NEK2 antibody

Alternative names for NEK2 antibody

NLK1, NimA-related protein kinase 2, HSPK 21, Serine/threonine-protein kinase Nek2

SwissProt ID

O35942 (Mouse), P51955 (Human)

Gene ID

4751 (NEK2)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of NEK2 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEK2 antibody

  Rabbit anti-NEK2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using NEK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEK2 antibody

  Rabbit polyclonal anti-NEK2 antibody  
Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein.  The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.  Personal communication Steven Pelech, Kinexus Inc. Rabbit Aff - Purified Hu E
0.1 mg / €356.00
  OriGene Technologies, Inc.

NEK2 antibody

  Mouse Monoclonal anti-NEK2 Antibody  
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed monoclonal antibody against NEK-2 (clone 8A6). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 8A6 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NEK2 antibody

  Rabbit Polyclonal Anti-NEK2 Antibody  
Host: Rabbit; Target Name: NEK2; Sample Tissue: HT1080 lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NEK2 antibody

  Rabbit Polyclonal Anti-NEK2 Antibody  
Host: Rabbit; Target Name: NEK2; Sample Tissue: Thyroid Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NEK2 antibody

  Rabbit Polyclonal Anti-NEK2 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Lovo cells, Primary antibody: (NEK2 Antibody) at dilution 1/727, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NEK2 (331-445) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2F6 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

NEK2 antibody

Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NEK2 antibody

The anti-NEK2 Pab is used in Western blot to detect NEK2 in HeLa cell lysate. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NEK2 antibody

Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

NEK2 - human gene knockout kit via CRISPR

NEK2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NEK2 - human gene knockout kit via CRISPR

NEK2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NEK2 - human gene knockout kit via CRISPR

NEK2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NEK2 - human gene knockout kit via CRISPR

NEK2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NEK2 (mGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, NEK2 (mGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, NEK2 (Myc-DDK tagged) - Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, NEK2 (Myc-DDK tagged) - Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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