NEK4 antibody

Principal name

NEK4 antibody

Alternative names for NEK4 antibody

NimA-related protein kinase 4, STK2, Serine/threonine-protein kinase Nek4, Serine/threonine-protein kinase 2, Serine/threonine-protein kinase NRK2

SwissProt ID

A5YM70 (Human), P51957 (Human), Q9Z1J2 (Mouse)

Gene ID

6787 (NEK4)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NEK4 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NEK4 antibody

Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 7.5% SDS PAGE. TA308520 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NEK4 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NEK4 antibody

NEK4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NEK4 antibody

Detection limit for recombinant GST tagged NEK4 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 1B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NEK4 antibody

NEK4 Mouse IgG2a 1B8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

NEK4 antibody

Detection limit for recombinant GST tagged NEK4 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 2E9 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

NEK4 antibody

NEK4 Mouse IgG2a 2E9 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

NEK4 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with NEK4 polyclonal antibody ( Cat # PAB3286 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NEK4 antibody

Western Blot: NEK4 Antibody [NBP1-42686]-Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-NEK4 antibody used for IP at 3 mcg/mg lysate.  NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42687, which recognizes a downstream epitope. Rabbit Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

NEK4 antibody

Western Blot: NEK4 Antibody [NBP1-42687]-Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42686, which recognizes an upstream epitope. Rabbit Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NEK4 antibody

Immunohistochemistry-Paraffin: NEK4 Antibody [NBP1-82528] - Immunohistochemical staining of human cerebral cortex shows strong nuclear and moderate cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NEK4

NEK4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

NEK4

NEK4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

NEK4

NEK4 Human
5 µg / €520.00
  Abnova Taiwan Corp.

14 Item(s)

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